In this paper, we uncover a new method for the preparation of biopolymer scaffolds and demonstrate its potential for the development of organs with the aid of tissue engineering. Two novel nanocomposite polymers, a nonbiodegradable polyhedral oligomeric silsesquioxane-poly (carbonate-urea)urethane and a biodegradable polyhedral oligomeric silsesquioxane-polycaprolactone-poly(carbonate-urea)urethane, have been subjected to flow in an electric field. Electrically forced microthreading of the polymers occurs and a three-dimensional print-patterning device was used to deposit fine (<50 µm) threads of polymer according to a predesigned architecture to prepare scaffolds. The technique can offer tremendous potential in the development of organs.
Ikada, Y. (2006). Tissue Engineering: Fundamentals and Applications , Academic Press, Boston, USA.
2.
Langer, R. and Vacanti, J.P. (1993). Tissue engineering, Science, 260: 920—926.
3.
Mooney, D.J. , Baldwin, D.F., Suh, N.P., Vacanti, J.P., and Langer, R. (1996). Novel approach to fabricate porous sponges of poly(d,l-lacticcoglycolic acid) without the use of organic solvents, Biomaterials, 17: 1417—1422.
4.
Yang, S., Leong, K.F., Du, Z., and Chua, C.K. (2001). The design of scaffolds for use in tissue engineering. Part I. Traditional factors, Tissue Eng., 7: 679—689.
5.
Draghi, L., Resta, S., Pirozzolo, M.G., and Tanzi, M.C. (2005). Microspheres leaching for scaffold porosity control , J. Mater. Sci. Mater. Med., 16: 1093—1097.
6.
Hosseinkhani, H., Mohsen Hosseinkhani , M., and Hisatoshi Kobayashi, H. ( 2006). Design of tissue-engineered nanoscaffold through self-assembly of peptide amphiphile, J. Bioact. Comp. Polym., 21: 277—296.
7.
Hou, Q., Grijpma, D.W., and Feijen, J. (2003). Preparation of interconnected highly porous polymeric structures by a replication and freeze-drying process, J. Biomed. Mater. Res. B Appl. Biomater.67: 732—740.
8.
Harris, L.D., Kim, B.S., and Mooney, D.J. (1998). Open pore biodegradable matrices formed with gas foaming, J. Biomed. Mater. Res., 42: 396—402.
9.
Nam, Y.S., Yoon, J.J., and Park, T.G. (2000). A novel fabrication method of macroporous biodegradable polymer scaffolds using gas foaming salt as a porogen additive, J. Biomed. Mater. Res., 53: 1—7.
10.
Taboas, J.M., Maddox, R.D., Krebsbach, P.H., and Hollister, S.J. (2003). Indirect solid free form fabrication of local and global porous, biomimetic and composite 3D polymer-ceramic scaffolds, Biomaterials, 24: 181—194.
11.
Hutmacher, D.W. (2001). Scaffold design and fabrication technologies for engineering tissues — state of the art and future perspectives , J. Biomater. Sci. Polym. Ed., 12: 107—124.
12.
Kim, S.S., Utsunomiya, H., Koski, J.A., Wu, B.M., Cima, M.J., Sohn, J., Mukai, K., Griffith, L.G., and Vacanti, J.P. (1998). Survival and function of hepatocytes on a novel three-dimensional synthetic biodegradable polymer scaffold with an intrinsic network of channels, Ann. Surg., 228: 8—13.
13.
Seitz, H., Rieder, W., Irsen, S., Leukers, B., and Tille, C. (2005). Three-dimensional printing of porous ceramic scaffolds for bone tissue engineering, J. Biomed. Mater. Res. B Appl. Biomater., 74: 782—788.
14.
Berkland, C. , Pack, D.W., and Kim, K.K. (2004). Controlling surface nanostructure using flow-limited field-injection electrostatic spraying (FFESS) of poly(d,l-lactide-co-glycolide), Biomaterials, 25: 5649—5658.
15.
Yeo, L.Y., Gagnon, Z., and Chang, H.C. (2005). AC electrospray biomaterials synthesis, Biomaterials, 26: 6122—6128.
16.
Bhattarai, S.R., Bhattarai, N., Viswanathamurthi , P., Yi , H.K., Hwang, P.H., and Kim, H.Y. (2006). Hydrophilic nanofibrous structure of polylactide; fabrication and cell affinity , J. Biomed. Mater. Res. A, 78: 247—257.
17.
Mo, X.M. , Xu, C.Y., Kotaki, M., and Ramakrishna , S. (2004). Electrospun P(LLA-CL) nanofiber: a biomimetic extracellular matrix for smooth muscle cell and endothelial cell proliferation, Biomaterials , 25: 1883—1890.
18.
Gratson, G.M. , Xu, M., and Lewis, J.A. (2004). Microperiodic structures: direct writing of three-dimensional webs, Nature, 428: 386.
19.
Buckley, C.T. and O'Kelly, K.U. (2004). Regular scaffold fabrication techniques for investigations in tissue engineering. In: Topics in Biomechanical Engineering, Prendergast, P.J. and McHugh, P.E. (eds), Dublin & Galway, pp. 147—166.
20.
Rayleigh, L. (1878). On the instability of jets , Proc. London Math. Soc., 11: 4—13.
Taylor, G.I. (1964). Disintegration of water droplets in an electric field, Proc. R. Soc. Lond. BBiol. Sci., 280: 383—397.
23.
Teng, W.D., Huneiti, Z.A., Machowski, W., Evans, J.R.G., Edirisinghe, M.J., and Balachandran, W. (1997). Towards particle-by-particle deposition of ceramics using electrostatic atomization, J. Mater. Sci. Lett., 16: 1017—1019.
24.
Cloupeau, M. and Prunet-Foch, B. (1990). Electrostatic spraying of liquids — main functioning modes, J. Electrostat., 25: 165—184.
25.
Jaworek, A. and Krupa, A. (1999). Classification of the modes of EHD spraying, J. Aerosol. Sci., 30: 873—893.
26.
Hartman, R.P.A. , Borra, J.P., Brunner, D.J., Marijnissen, J.C.M., and Scarlett , B. (1999). The evolution of electro-hydrodynamic sprays produced in the cone-jet mode, a physical model, J. Electrostat., 47: 143—170.
27.
GananCalvo, A.M., Davila, J., and Barrero, A. (1997). Current and droplet size in the electrospraying of liquids. Scaling laws, J. Aerosol Sci., 28: 249—275.
28.
Hartman, R.P.A. , Brunner, D.J., Camelot, D.M.A., Marijnissen , J.C.M., and Scarlett, B. (2001). Jet break-up in electro-hydrodynamic atomization in the cone-jet mode, J. Aerosol Sci., 31: 65—95.
29.
Edirisinghe, M.J. and Jayasinghe , S.N. (2004). Electro-hydrodynamic atomization of a concentrated nano-suspension, Int. J. Appl. Ceram. Tech., 1: 140—145.
30.
Jayasinghe, S.N., Edirisinghe, M.J., and De Wilde , T. (2002). A novel ceramic printing technique based on electrostatic atomization of a suspension, Mater. Res. Innov., 6: 92—95.
31.
Wang, D.Z., Jayasinghe, S.N., and Edirisinghe, M.J. (2005). High resolution print-patterning of a nano-suspension, J. Nanopart. Res., 7: 301—306.
Shin, M., Hohman, M.M., Brenner, G.C., and Rutledge, A. (2001). A whipping fluid jet generates submicron polymer fibres, Appl. Phys. Lett., 78: 1149—1151.
34.
Doshi, J. and Reneker, D.H. (1995). Electrospinning process and applications of electrospun fibres, J. Electrostat., 35: 151—160.
35.
Sigmund, W. , Yuh, J., Park, H., Maneeratana, V., Pyrgiotakis, G., Daga, A., Taylor, J., and Nino, J.C. (2006). Processing and structure relationships in electrospinning of ceramic fibre systems, J. Am. Ceram. Soc., 89: 395—407.
36.
Zhang, H.B. and Edirisinghe, M.J. (2006). Electrospinning zirconia fibre from a suspension, J. Am. Ceram. Soc., 89: 1870—1875.
37.
Zhang, H.B., Jayasinghe, S.N., and Edirisinghe, M.J. (2006). Electrically forced microthreading of highly viscous dielectric liquids, J. Electrostat., 64: 355—360.
38.
Mironov, V., Reis, N., and Derby, B. (2006). Bioprinting: a beginning, Tissue Eng., 12: 631—634.
39.
Jakab, K., Damon, B., Neagu, A., Kachurin, K., and Forgacs, G. (2006). Three dimensional tissue constructs built by bioprinting , Biorheology, 43: 509—513.
40.
Kannan, R.Y., Salacinski, H.J., De, G.J., Clatworthy, I., Bozec, L., Horton, M., Butler, P.E., and Seifalian, A.M. (2006). The antithrombogenic potential of a polyhedral oligomeric silsesquioxane (POSS) nanocomposite, Biomacromolecules , 7: 215—223.
41.
Kannan, R.Y., Salacinski, H.J., Butler, P.E., and Seifalian, A.M. (2005). Polyhedral oligomeric silsesquioxane nanocomposites: the next generation material for biomedical applications, Acc. Chem. Res., 38: 879—884.
42.
Kannan, R.Y. , Salacinski, H.J., Odlyha, M., Butler, P.E., and Seifalian , A.M. (2006). The degradative resistance of polyhedral oligomeric silsesquioxane nanocore integrated polyurethanes: an in vitro study, Biomaterials , 27: 1971—1979.
43.
Lee, Y.J., Kuo, S.W., Huang, W.J., Lee, H.Y., and Chang, F.C. (2004). Miscibility, specific interactions, and self-assembly behavior of phenolic/polyhedral oligomeric silsesquioxane hybrids, J. Polym. Sci. BPolym. Phys., 42: 1127—1136.
44.
Zhang, H.B. , Edirisinghe, M.B., and Jayasinghe , S.N. (2006). Flow behaviour of dielectric liquids in an electric field, J. Fluid Mech., 558: 103—111.
45.
Bianchi, F. , Vassalle, C., Simonetti, M., Vozzi, G., Domenici, C., and Ahluwalia, A. (2006). Endothelial cell function on 2D and 3D microfabricated polymer scaffolds: applications in cardiovascular tissue engineering, J. Biomater. Sci. Polym. Ed., 17: 37—51.
46.
Mariani, M. , Rosatini, F., Vozzi, G., Previti, A., and Ahluwalia, A. (2006). Characterization of tissue-engineered scaffolds microfabricated with PAM, Tissue Engineering12: 547—557.
47.
Choi, R.S. and Vacanti, J.P. (1997). Preliminary studies of tissue-engineered intestine using isolated epithelial organoid units on tubular synthetic biodegradable scaffolds, Transplant. Proc., 29: 848—851.
48.
Duxbury, M.S. , Grikscheit, T.C., Gardner-Thorpe , J., Rocha, F.G., Ito, H., Perez, A., Ashley, S.W., Vacanti, J.P., and Whang, E.E. (2004). Lymphangiogenesis in tissue-engineered small intestine, Transplantation , 77: 1162—1166.
49.
Kaihara, S. , Kim, S., Benvenuto, M., Kim, B.S., Mooney, D.J., Tanaka, K., and Vacanti, J.P. (1999). End-to-end anastomosis between tissue-engineered intestine and native small bowel, Tissue Eng., 5: 339—346.
50.
Kim, S.S., Kaihara, S., Benvenuto, M.S., Choi, R.S., Kim, B.S., Mooney, D.J., Taylor, G.A., and Vacanti, J.P. (1999). Regenerative signals for intestinal epithelial organoid units transplanted on biodegradable polymer scaffolds for tissue engineering of small intestine, Transplantation, 67: 227—233.
51.
Kim, S.S., Kaihara, S., Benvenuto, M.S., Choi, R.S., Kim, B.S., Mooney, D.J., and Vacanti, J.P. (1999). Effects of anastomosis of tissue-engineered neointestine to native small bowel, J. Surg. Res., 87: 6—13.
52.
Vacanti, J.P. , Morse, M.A., Saltzman, W.M., Domb, A.J., Perez-Atayde , A., and Langer, R. (1998). Selective cell transplantation using bioabsorbable artificial polymers as matrices, J. Pediatr. Surg., 23: 3—9.
