Evaluation of Cell Adhesion and Proliferation on a Novel Tissue Engineering Scaffold Containing Chitosan and Hydroxyapatite

Abstract

Porous chitosan-hxdroxyapatite hybrids were developed by partial enzymatic degradation [14] of the chitin/chitosan surface using chitosanase and lysozyme. This article evaluates the influence of chitosan and hydroxyapatite components, substrate roughness, stability, as well as surface porosity produced by enzymatic hydrolysis to cell adhesion and proliferation. L929 mouse fibroblastic lung cells were cultured on enzymatic degraded porous chitosan-hydroxyapatite hybrids. The presence of hydroxyapatite and porosity produced by partial lysozyme hydrolysis enhance cell proliferation. Besides, cell adhesion and proliferation are primarily dependent on substrate roughness and stability.

Keywords

porous chitosan-hydroxyapatite enzymatic hydrolysis biocompatibility

Get full access to this article

View all access options for this article.

References

Aebischer, P. , Goddard, M.B. , Sasken, H.F. , Hunter, T.J. and Galletti, P.M. (1988). Biomaterials, 9: 80-80.

Deible, C.R. , Petrosko, P. , Johnson, P.C. , Beckman, E.J. , Russell, A.J. and Wagner, W.R. (1999). Biomaterials, 20: 101-101.

Dion, I. , Roques, X. , More, N. , Labrousse, L. , Caix, J. , Lefebvre, F. , Rouais, F. , Gautreau, J. and Baquey, C. (1993). Biomaterials, 14: 712-712.

Feuerstein, I.A. and Ratner, B.D. (1990). Biomaterials, 11: 127-127.

Desai, N.P. and Hubbell, J.A. (1989). J. Biomater. Sci. Polym. Ed., 1: 123-123.

Layman, D.L. and Ardoin, R.C. (1998). J. Biomed. Mater. Res., 40: 282-282 .

Gloecker, D.C. , Sacks, M.S. , Billiar, L.K. and Bachrach, N. (2000). J. Biomed. Mater. Res., 52: 365-365 .

Hu, Q. , Li, B. , Wang, M. and Shen, J. (2004). Biomaterials, 25: 779-779 .

Lo , W.J. , Grant, D.M. , Ball, B.M. , Welsh, D.S. , Howdle, S.M. and Antonov, E.N. (2000). J. Biomed. Mater. Res., 50: 536-536 .

10.

Craig, R.G. and LeGeros, R.Z. (1999). J. Biomed. Mater. Res., 47: 585-585.

11.

Ghannam, A.E. , Ducheyne, P. and Shapiro, I.M. (1997). J. Biomed. Mater. Res., 36: 167-167.

12.

Vogt, S. , Larcher, Y. , Beer, B. , Wilke, I. and Schnabelrauch, M. (2002). European Cells and Materials, 4:30-30.

13.

Tachaboonyakiat, W. , Serizawa, T. and Akashi, M. (2001). Polym. J., 33: 177-177.

14.

Tachaboonyakiat, W., Serizawa, T. and Akashi, M. (2002). J. Biomater. Sci. Polym. Ed., 13: 1021.

15.

Beppu, M.M. and Santana, C.C. (2002). Materials Research, 5: 47-47.

16.

Izume, M. , Taira, T. , Kimura, T. and Miyata, T. (1989). “A novel cell culture matrix composed of chitosan and collagen complex”, In: Skjak-Braek, G. , Anthonsen, T. and Sandford, P. (eds), Chitin and Chitosan, p. 653–666, Elsevier Applied Science, Amsterdam .

17.

Thomson, R.C. , Wake, M.C. , Taszemski, M. and Mikos, A.G. (1995). Adv. Polym. Sci., 122: 247-247.

18.

Langer, R. (1999). J. Control. Release, 62: 7-7.

19.

Zhang, R. and Ma, P.X. (1999). J. Biomed. Mater. Res., 44: 446-446 .

20.

Shapiro, L. and Cohen, S. (1997). Biomaterials, 18: 583-583 .

21.

Saad, B. , Cairdelli, G. and Matter, S. (1998). J. Biomed. Mater. Res., 39: 594-594 .

22.

Iio, K. , Minoura, M. , Aiba, S. , Nagura, M. and Kodama, M. (1994). J. Biomed. Mater. Res., 28: 459-459 .

23.

Lee, J.H. , Khang, G. , Lee, J. W. , Lee, H.B. (1998). J. Colloid Interface Sci., 205: 323-323 .

24.

Narita, T. , Harai, A. , Xu, J. , Gong, J.P. and Osada, Y. (2000). Biomacromolecules, 1: 162-162 .