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Abstract

Porous chitosan-hxdroxyapatite hybrids were developed by partial enzymatic degradation [14] of the chitin/chitosan surface using chitosanase and lysozyme. This article evaluates the influence of chitosan and hydroxyapatite components, substrate roughness, stability, as well as surface porosity produced by enzymatic hydrolysis to cell adhesion and proliferation. L929 mouse fibroblastic lung cells were cultured on enzymatic degraded porous chitosan-hydroxyapatite hybrids. The presence of hydroxyapatite and porosity produced by partial lysozyme hydrolysis enhance cell proliferation. Besides, cell adhesion and proliferation are primarily dependent on substrate roughness and stability.

Keywords

porous chitosan-hydroxyapatite enzymatic hydrolysis biocompatibility

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Evaluation of Cell Adhesion and Proliferation on a Novel Tissue Engineering Scaffold Containing Chitosan and Hydroxyapatite

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