A comparison of sleep organization in Roman high-(RHA/Verh) and low-(RLA/Verh) avoidance rats, which differ in the way they respond to environmental stimuli and in several neuroendocrine and neurochemical parameters, was carried out. EEG-sleep recordings were obtained from adult males over 12:12 light-dark periods to determine how these two psychogenetically selected rat lines might also differ in their sleep-wake cycle. There was no significant difference in total sleep time between the two lines. However, the (hypoemotional) RHA/Verh rats showed an overall increase (percentage of total sleep) in paradoxical sleep (PS) duration, with a concomitant decrease in slow-wave sleep (SWS). During the dark phase, RHA/Verh rats showed a shorter PS latency and a larger number of PS episodes. Hourly sleep scoring also revealed a more discontinuous pattern (total sleep and PS vs. SWS) during the dark phase in RHA/Verh rats. In relation to recognized neurochemical and neuroendocrine differences between them, these rat lines may prove useful in investigations of the neurobiological mechanisms underlying sleep regulation.
Aubry JM , Bartanusz V, Driscoll P, Schulz P, Steimer T, and Kiss JZ (1995) Corticotropin-releasing factor and vasopressin mRNA levels in Roman high-and low-avoidance rats: Response to open-field exposure. Neuroendocrinology61:89-97.
2.
Bignami G (1965) Selection for high rates and low rates of avoidance conditioning in the rat. Anim Behav13:221-227.
3.
Bjorvath B and Ursin R (1994) Effects of the selective 5-HT1Bagonist, CGS12066B, on sleep/waking stages and EEG power spectrum in rats. J Sleep Res3:97-105.
4.
Castanon N and Mormède P (1994) Psychogenetics: Adapted tools for the study of the coupling between behavioral and neuroendocrine traits of emotional reactivity. Psychoneuroendocrinology19:257-282.
5.
Cespuglio R , Sarda N, Gharib A, Houdouin F, Rampin C, and Jouvet M (1990) Voltammetric detection of the release of 5-hydroxyindole compounds throughout the sleep-waking cycle of the rat. Exp Brain Res80:121-128.
6.
Cools AR , Brachten R, Heeren D, Willemin A, and Ellen-broek B (1990) Search after neurobiological profile of individual-specific features of Wistar rats. Brain Res Bull24:49-69.
7.
D’Angio M , Serrano A, Driscoll P, and Scatton B (1988) Stressful environmental stimuli increase extracellular DOPAC levels in the prefrontal cortex of hypoemotional (Roman high-avoidance) but not hyperemotional (Roman low-avoidance) rats: An in vivo voltammetric study. Brain Res451:237-247.
8.
Decobert M , Driscoll P, Riou-Merle F, and Depoortere H (1986) Pharmaco-EEG studies in Roman high-and low-avoidance (RHA/Verh and RLA/Verh) rats. Neurosci Lett26(Suppl.):S132-S132.
9.
De Saint Hilaire-Kafi S , Hjorth S, and Gaillard JM (1987) Effects of 8-OH-DPAT on the sleep-waking cycle in the rat. In Brain 5-HT1A Receptors: Behavioral and Neurochemical Pharmacology, CT Dourish, S Ahlenius, and PH Huston, eds, pp 135-139, Ellis-Horwood, Chichester, UK.
10.
Driscoll P (1988) Hypothalamic serotonin turnover in rat lines selectively bred for differences in active, two-way avoidance behavior. Adv Biosci70:55-58.
11.
Driscoll P and Bättig K (1982) Behavioral, emotional and neurochemical profiles of rats selected extreme differences in active, two-way avoidance performance. In Genetics of the Brain, I Lieblich, ed, pp 95-123, Elsevier, Amsterdam.
12.
Driscoll P , Dedek J, D’Angio M, Claustre Y, and Scatton BA (1990) Genetically-based model for divergent stress responses: Behavioral, neurochemical and hormonal aspects. In Farm Animals in Biomedical Research, V Pliska and G Stranzinger, eds, pp 97-107, Verlag Paul Parey, Hamburg, Germany.
13.
Driscoll P , Dedek J, Martin JR, and Zivkovic B (1983) Twoway avoidance and acute shock stress induced alterations of regional noradrenergic, dopaminergic and serotonergic activity in Roman high-and low-avoidance rats. Life Sci33:1719-1725.
14.
Driscoll P , Escorihuela RM, Fernández-Teruel A, Giorgi O, Schwegler H, Steimer Th, Wiersma A, Corda MJ, Flint J, Koolhaas JM, Langhans W, Schulz PE, Siegel J, and Tobeña A (1998) Genetic selection and differential stress responses. Ann NY Acad Sci851:501-510.
15.
Driscoll P , Gentsch C, Lichtsteiner M, and Feer H (1985) Selection for extreme differences in two-way avoidance: Is anxiety and/or depression involved? In Biological Psychiatry 1985, C Shagass et al., eds, pp 34-36, Elsevier, Amsterdam.
16.
Ferré P , Fernández-Teruel A, Escorihuela RM, Driscoll P, Corda MG, Giorgi O, and Tobeña A (1995) Behavior of the Roman/Verh high-and low-avoidance rat lines in anxiety tests: Relationship with defecation and self-grooming. Physiol Behav58:1209-1213.
17.
Gaillard JM (1990) Le sommeil: Ses mécanismes et ses troubles, Lausanne, Payot.
18.
Gentsch C , Lichtsteiner M, Driscoll P, and Feer H (1982) Differential hormonal and physiological responses to stress in Roman high-and low-avoidance rats. Physiol Behav28:259-263.
19.
Gentsch C , Lichtsteiner M, and Feer H (1991) Genetic and environmental influences on reactive and spontaneous behavioral locomotor in rats. Experientia47:998-1008.
20.
Giorgi O , Orlandi M, Escorihuela RM, Driscoll P, Lecca D, and Corda MG (1994) GABAergic and dopaminergic transmission in the brain of Roman high-avoidance and Roman low-avoidance rats. Brain Res638:133-138.
21.
Gué M , Alary C, Del Rio-Lacheze C, Junien JL, and Búeno L (1993) Comparative involvement of 5-HT1, 5-HT2 and 5-HT3 receptors in stress-induced colonic motor alterations in rats. Eur J Pharmacol233:193-199.
22.
Houdouin F , Cespuglio R, Gharib N, Sarda N, and Jouvet M (1991) Detection of the release of 5-hydroxyindole compounds in the hypothalamus and the raphe dorsalis throughout the sleep-waking cycle and during stressful situations in the rat. A polygraphic and voltammetric approach. Exp Brain Res85:153-162.
23.
Jouvet M (1969) Biogenic amines and state of sleep. Science163:32-41.
24.
Kleinlogel H and Driscoll P (1984) EEG-sleep studies in Roman high-and low-avoidance (RHA/Verh and RLA/ Verh) rats before and after neuroleptic treatment. Neurosci Letters18(Suppl.):S367-S367.
25.
Morgane PJ and Stern WC (1972) Relationship of sleep to neuroanatomical circuits, biochemistry, and behavior. Ann NY Acad Sci193:95-111.
26.
Rosenberg RS , Bergman BM, Son HJ, Arnason BGW, and Rechtschaffen A (1987) Strain differences in the sleep of rats. Sleep10:537-541.
27.
Rossi R , Driscoll P, and Langhans W (1997) Differential circadian eating patterns in two psychogenetically selected strains of rats fed low-, medium-and high-fat diets. Behav Genet27:565-572.
28.
Schreiber R and de Vry J (1993) 5-HT1A receptor ligands in animal models of anxiety, impulsivity and depression: multiple mechanisms of action?Prog Neuro-Psychopharmacol Biol Psychiat17:87-104.
29.
Steimer Th , Driscoll P, and Schulz P (1997a) Brain metabolism of progesterone, coping behavior and emotional reactivity in male rats from two psychogenetically selected lines. J Neuroendocrinol9:169-175.
30.
Steimer Th , la Fleur S, and Schulz P (1997b) Neuroendocrine correlates of emotional reactivity and coping in male rats from the Roman high (RHA/Verh)-and low (RLA/ Verh)-avoidance lines. Behav Genet27:503-512.
31.
Valatx JL , Bugat R, and Jouvet M (1972) Genetic studies of sleep in mice. Nature238:226-227.
