S-Antigen Antibody Partially Blocks Entrainment and the Effects of Constant Light on the Circadian Rhythm of Locomotor Activity in the Adult Blow Fly,Calliphora vicina
Free accessResearch articleFirst published online March, 1996
S-Antigen Antibody Partially Blocks Entrainment and the Effects of Constant Light on the Circadian Rhythm of Locomotor Activity in the Adult Blow Fly,Calliphora vicina
Injection of S-antigen (arrestin) antibody into the brain of the blow fly Calliphora vicina, appeared to reduce sensitivity to the photic effects of a light:dark cycle (LD; entrainment) or continuous "bright" light (LL; arrhythmicity). In LD, a proportion of the injected flies evaded entrainment or showed delayed entrainment. In bright LL, flies continued with a free-running rhythm, which remained unchanged (as in continuous darkness) or lengthened (as in "dim" LL). These results focus attention on four groups of arrestin-positive neurons in the fly's brain as potential components of the photoreceptive system.
Bentrop J., Plangger A., and Paulsen R. (1993) An arrestin homolog of blowfly photoreceptors stimulates visual-pigment phosphorylation by activating a membrane-associated protein kinase. Eur J Biochem216:67-73.
2.
Binkley S. (1993) Structures and molecules involved in generation and regulation of biological rhythms in vertebrates and invertebrates. Experientia49:648-653.
3.
Cymborowski B. and Korf H-W. (1995) Immunocytochemical demonstration of S-antigen (arrestin) in the brain of the blowfly Calliphora vicina. Cell Tissue Res279:109-114.
4.
Cymborowski B., Lewis Rd, Hong S-F., and Saunders Ds (1994) Circadian locomotor activity rhythms and their entrainment to light-dark cycles continue in flies (Calliphora vicina) surgically deprived of their optic lobes. J Insect Physiol40:501-510.
5.
Enright Jt (1965) The search for rhythmicity in biological time-series . J Theor Biol8:426-468.
6.
Hong S-F. and Saunders Ds (1994) Effects of constant light on the rhythm of adult locomotor activity in the blow fly, Calliphora vicina. Physiol Entomol19:319-324.
7.
Kenny Nap and Saunders Ds (1991) Adult locomotor rhythmicity as "hands" of the maternal photoperiodic clock regulating larval diapause in the blowfly, Calliphora vicina. J Biol Rhythms6:217-233.
8.
Korf H-W. and Wicht H. (1992) Receptor and effector mechanisms in the pineal organ. In Progress in Brain Research, A Ermisch, R Landgraf, and HJ Ruhle, eds, pp 285-297, Elsevier Science , New York.
9.
Kuenzel Wj (1993) The search for deep encephalic photoreceptors within the avian brain, using gonadal development as a primary indicator. Poult Sci72:959-967.
10.
Menaker M. and Underwood H. (1976) Extraretinal photoreception in birds. Photochem Photobiol23:299-306.
11.
Mirshahi M., Boucheix C., Collenot G., Thillaye B., and Faure Jp (1985) Retinal S-antigen epitopes in vertebrate and invertebrate photoreceptors. Invest Ophthalmol Vis Sci26:1016-1021.
12.
Page Tl ( 1984) Neuronal organization of a circadian clock in the cockroach Leucophaea maderae. In Photoperiodic Regulation of Insect and Molluscan Hormones (Vol 104, pp 115-135), Ciba Foundation Symposium, Pitman , London.
13.
Plangger A., Malicki D., Whitney M., and Paulsen R. (1994) Mechanism of arrestin 2 function in rhabdomeric photoreceptors. J Biol Chem269:26969-26975.
14.
Saunders Ds (1987) Maternal influence on the incidence and duration of larval diapause in Calliphora vicina. Physiol Entomol12:331-338.
15.
Schwemer J. (1984) Renewal of visual pigment in photoreceptors of the blowfly. J Comp Physiol A 154:535-547.
16.
Truman Jw (1976) Extraretinal photoreception in insects. Photochem Photobiol23:215-225.
17.
Van Veen T., Eloffsson R., Hartwig Hg, Gery I., Mochizuki M., and Klein Dc (1986) Retinal S-antigen: Immunocytochemical and immunochemical studies on the distribution in animal photoreceptors and pineal organs. Exp Biol45:15-25.
18.
Williams Ja and Naylor E. (1978) A procedure for the assessment of significance of rhythmicity in time-series data. Int J Chronobiol5:435-444.
