Exposure to hexavalent chromium Cr(VI) compounds is of concern in many Cr-related industries and their surrounding environments. K2Cr2O7 is widely recognized as an animal and human carcinogen, mutagen, and teratogen. The present study investigated the bone maturity of suckling rats whose mothers were treated with K2Cr2O7. Experiments were carried out on female Wistar rats given 700 ppm of K2Cr2O7 in their drinking water from the 14th day of pregnancy until day 14 after delivery. Exposing dams to K2Cr2O7 caused disorders in the bone of their progeny. As corollary to this, malondialdehyde levels increased, while glutathione, a non-protein thiol and vitamin C decreased. Alteration of the antioxidant system in the treated group was also confirmed by the significant decline of superoxide dismutase, catalase, and glutathione peroxidase activities. Furthermore, K2Cr2O7 induced changes in bone mineralization, especially calcium and phosphorus levels, which decreased. Whereas, in plasma and urine, they increased and decreased inversely. These results suggest that K2Cr2O7 accelerated bone resorption activity. In fact, in treated pups, total tartrate-resistant acid phosphatase, which reflected bone resorption, was enhanced while total alkaline phosphatase, which reflected bone formation, was reduced. The impairment of bone function was corresponded histologically.
Aebi H. ( 1984) Catalase in vitro. Methods in Enzymology105: 121-126.
2.
Anane R. and Creppy EE ( 2001) Lipid peroxidation as pathway of aluminium cytotoxicity in human skin fibroblast cultures: prevention by superoxide dismutase + catalase and Vitamins E and C. Human Experimental Toxicology20: 477-481.
3.
Arai M., Shibata Y., Pugdee K., Abiko Y., and Ogata Y. ( 2007) Effects of reactive oxygen species (ROS) on antioxidant system and osteoblastic differentiation in MC3T3-E1 cells. IUBMB Life59: 27-33.
4.
Badary OA, Abdel-Maksoud S., Ahmed WA, and Owieda GH ( 2004) Naringenin attenuates cisplatin nephrotoxicity in rats . Life Science76: 2125-2135.
5.
Bagchi D., Balmoori J., Bagchi M., et al. (2002) Comparative effects of TCDD, endrin, naphthalene and chromium (VI) on oxidative stress and tissue damage in the liver and brain tissues of mice. Toxicology175: 73-82.
6.
Bagchi D., Vuchetich PJ, Bagchi M., Hassoun EA, Tran MX, Tang L., and Stohs SJ ( 1997) Induction of oxidative stress by chronic administration of sodium dichromate [chromium VI] and cadmium chloride [cadmium II] to rats . Free Radical Biology & Medicine22:471-478.
7.
Ball LM and Chhabra RS ( 1981) Intestinal absorption of nutrients in rats treated with 2,3,7,8 tetrachlorodi benz-p-dioxin (TCDD). Journal of Toxicology and Environmental Health8: 629-636.
8.
Barceloux D. ( 1999) Chromium. Journal of Toxicology and Clinical Toxicology37: 173-194.
9.
Beauchamp C. and Fridovich I. ( 1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gel. Analytical Biochemistry44: 276-287.
10.
Blacksmith Institute ( 2007) Top 10 worst polluted sites. The Blacksmith Institute, New York: carcinogenesis. Free Radical Biology and Medicine37: 582-593.
11.
Brzóska MM and Moniuszko-Jakoniuk J. ( 2005) Disorders in bone metabolism of female rats chronically exposed to cadmium. Toxicology and Applied Pharmacology202: 68-83.
12.
Costa HM, Rodrigues RC, Mattos Mda G., and Ribeiro RF ( 2003) Evaluation of the adaptation interface of one-piece implant-supported superstructures obtained in Ni-Cr-Ti and Pd-Ag alloys. Brazilian Dental Journal14: 197-202.
13.
Council of European Communities (1986) Council instructions about the protection of living animals used in scientific investigations. Official Journal of the European Communities (JO 86/609/CEE) L358: 1-18.
14.
Draper HH and Hadley M. ( 1990) Malondialdehyde determination as index of lipid peroxidation . Methods in Ezymology86: 421-431.
15.
Ellman GL ( 1959) Tissue sulfhydryl groups. Archives in Biochemistry and Biophysics82: 70-77.
16.
Fatima S., Arivarasu NA, Banday AA, Yusufi AN, and Mahmood R. ( 2005) Effect of potassium dichromate on renal brush border membrane enzymes and phosphate transport in rats. Human Experimental Toxicology24: 631-663.
17.
Fishbeck K. and Rasmussen KM ( 1987) Effect of repeated cycles on maternal nutritional status lactational performance and litter growth in ad libitum fed and chronically food restricted rats. Journal of Nutrition117: 1967-1975.
18.
Flohe L. and Gunzler WA ( 1984) Assays of glutathione peroxidase. Methods in Enzymology105: 114-121.
19.
Fukino H., Hirai M., Hsueh YM, Moriyasu S., and Yamane Y. ( 1986) Mechanism of protection by zinc against mercuric chloride toxicity in rats: effects of zinc and mercury on glutathionine metabolism . Journal of Toxicology and Environmental Health19: 75-89.
20.
Gabe M. ( 1968) Techniques histologiques. Paris : Masson, 838-841.
21.
Gadd GM and White C. ( 1993) Microbial treatment of metal pollution a working biotechnology . Trends in Biotechnology11: 353-359.
22.
Garrett JR, Boyce BF, Oreffo RO, Bonewald L., Poser J., and Mundy GR ( 1990) Oxygen-derived free radicals stimulate osteoclastic bone resorption in rodent bone in vitro and in vivo. Journal of Clinical Investigation85: 632-639.
23.
Gdula-Argasinska J., Appleton J., Sawicka-Kapusta K., and Spence B. ( 2004) Further investigation of the heavy metal content of the teeth of the bank vole as an exposure indicator of environmental pollution in Poland. Environmental Pollution131: 71-79.
24.
Iarc (1990) Monograph on the Evaluation of Carcinogenic Risk to Humans, Chromium, Nickel and Welding, Lyon, France: IARC.
25.
Ito H., Okamoto K., and Kato K. ( 1998) Enhancement of expression of stress proteins by agents that lower the levels of glutathione in cells. Biochemica Biophysica Acta1397: 223-230.
26.
Jacques-Silva MC, Nogueira CW, Broch LC, Flores EMM, and Rocha JBT ( 2001) Diphenyl diselenide and ascorbic acid changes deposition of selenium and ascorbic acid in liver and brain of mice. Pharmacology and Toxicology88: 119-125.
27.
Jollow DJ, Mitchell JR, Zampaglione N., and Gillette JR ( 1974) Bro-mobenzene-induced liver necrosis Protective role of glutathi-one and evidence for 3, 4-bromobenzene oxide as the hepato-toxic metabolite. Pharmacology11:151-169.
28.
Junaid M., Murthy RC, and Saxena DK ( 1995) Chromium fetotoxicity in mice during late pregnancy . Veterinary and Human Toxicology37: 320-323.
29.
Junaid M., Murthy RC, and Saxena DK ( 1996a) Embryo-toxicity of orally administered chromium in mice: exposure during the period of organogenesis. Toxicology Letters84: 143-148.
30.
Junaid M., Murthy RC, and Saxena DK ( 1996b) Embryo and fetotoxicity of chromium in pregestationally exposed mice. Bulletin of Environmental Contamination and Toxicology57: 327-334.
31.
Kanojia RK, Junaid M., and Murthy RC ( 1996) Chromium induced teratogenicity in female rat. Toxicology Letters89: 207-214.
32.
Kanojia RK, Junaid M., and Murthy RC ( 1998) Embryo and fetotoxicity of hexavalent chromium: a long-term study. Toxicology Letters95: 165-172.
33.
Lewicki M., Paez H., and Mandalunis PM ( 2006) Effect of lithium carbonate on subchondral bone in sexually mature Wistar rats. Experimental and Toxicologic Pathology58: 197-201.
34.
Lowry OH, Rosebrough NJ, Farr AL, and Randall RJ ( 1951) Protein measurement with the Folin phenol reagent . Journal of Biological Chemistry193: 265-275.
35.
Makarov Y. and Shimtova LA ( 1978) Occupational conditions and gynecological illness in workers engaged in the production of chromium compounds. Environmental Health Perspectives24: 1-128.
36.
Mertz W. ( 1995) Risk assessment of essential trace elements: new approaches to setting recommended dietary allowances and safety limits. Nutritional Reviews53: 179-185.
37.
Oliveira JTA, Silveira SB, Vasconcelos IM, Cavada BS, and Moriera RA ( 1999) Compositional and nutritional attributes of seeds from the multiple purpose tree Moringa oleifera Lamark. Journal of the Science of Food and Agriculture79: 815-820.
38.
Ott M., Gogvadze V., Orrenius S., and Zhivotovsky B. ( 2007) Mitochondria, oxidative stress, and cell death . Apoptosis12: 913-922.
39.
Ozgocmen S., Kaya H., Fadillioglu E., Aydogan R., and Yilmaz Z. ( 2007) Role of antioxidant systems, lipid peroxidation and nitric oxide in postmenopausal osteoporosis. Molecular and Cellular Biochemistry295: 45-52.
40.
Pedraza-Chaverri J., Yam-Canul Y., Chirino YI, et al. (2008) Protective effects of garlic powder against potassium dichromate-induced oxidative stress and nephrotoxicity. Food and Chemical Toxicology46: 619-627.
41.
Pribluda LA ( 1963) Chromium content of the long bones of rats at different stages of pregnancy. Dokl Akad Nauk Belrussk SSR7: 206-212.
42.
Pritchard KA, Ackerman A., and Kalyanaraman B. ( 2000) Chromium VI increases endothelial cell expression of ICAM-1 and decreases nitric oxide activity. The Journal of Environmental Pathology , Toxicology and Oncology19: 251-260.
43.
Ramajayam G., Sridhar M., Karthikeyan S., et al. (2007) Effects of Aroclor on femoral bone metabolism in adult male Wistar rats. Toxicology241: 99-105.
44.
Schwartz D. ( 1963) Méthodes statistiques à l’usage des médecins et des biologistes. [Statistical Methods for Doctor and Biologist’s Use]. Paris: Flammarion Publishers .
45.
Shmitova LA ( 1978) The course of pregnancy in women engaged in the production of chromium and its compounds. Sverdlovsk108-111 (Russian).
46.
Shmitova LA ( 1980) Content of hexavalent chromium in the biological substrates of pregnant women and women in the immediate postnatal period engaged in the manufacture of chromium compounds. Gig trud Prof Zabol2: 33-35 (Russian).
47.
Singhal RK, Anderson ME, and Meister A. ( 1987) Glutathione, a first line of defense against cadmium toxicity . The FASEB Journal1: 220-223.
48.
Sontakke AN and Tare RS ( 2002) A duality in the roles of reactive oxygen species with respect bone metabolism. Clinica Chimica Acta318: 145-148.
49.
Suzuki Y. ( 1990) Synergism of ascorbic acid and glutathione in the reduction of hexavalent chromium in vitro. Industrial Health28: 9-19.
50.
Talbot MS, Cifuentes M., Dunn MG, and Shapses SA ( 2001) Energy restriction reduces bone density and biomechanical proprieties in aged female rats. Journal of Nutrition131: 2382-2387.
51.
Tipton IH ( 1960) The distribution of trace metals in the human body . In: Seven MJ (ed.) Metal-Binding in Medicine. Philadelphia: Lippincott , 27.
52.
Trivedi B., Saxena DK, Murthy RC, and Chandra SV ( 1989) Embryotoxicity and fetotoxicity of orally administered hexavalent chromium in mice. Reproductive Toxicology3: 275-278.
53.
Ubios AM, Guglielmotti MB, Steimetz T., and Cabrini RL ( 1991) Uranium inhibits bone formation in physiologic alveolar bone modeling and remodeling. Environmental Research54: 17-23.
54.
Vahter M., Akesson A., Lide’n C., Ceccatelli S., and Berglund M. ( 2007) Gender differences in the disposition and toxicity of metals. Environmental Research104: 85-95.
55.
Witmer CM, Harris R., and Shupack SI ( 1991) Oral bioavailability of chromium from a specific site . Environmental Health Perspectives92: 105-110.
56.
Yang S., Madyastha P., Bingel S., Ries W., and Key L. ( 2001) A new superoxide-generating oxidase in murine osteoclasts . Journal of Biological Chemistry276: 5452-5458.
57.
Yousef MI, El-Demerdash FM, Kamil KI, and Elaswad FAM ( 2006) Ameliorating effect of folic acid on chromium (VI)-induced changes in reproductive performance and seminal plasma biochemistry in male rabbits. Reproductive Toxicology2: 322-328.
