Ammonia-oxidizing bacteria and phenol-degrading yeast were isolated in order to study the synergism effects of phenol-degrading yeast and ammonia-oxidizing bacteria for enhancing the nitrification in coke wastewater from the Isfahan Steel Company. The influent and effluent samples with ∼ 600—1200 mg L— 1 ammonium and 550—2350 mg L—1 phenol were collected aseptically in sterile flasks. The biodegradation of phenol and nitrification were studied with different treatments. The results showed that addition of Na2CO3 and autotrophic bacteria to wastewater increased the ammonium removal by 100%. Furthermore, the synergism effects of phenol-degrader yeast and autotrophic bacteria reduced the time for ammonium removal.
Aakra A., Utaker, J.B., Nes, I.F. & Bakken, L.R. (1999) An evaluated improvement of the extinction dilution method for isolation of ammonia-oxidizing bacteria. Journal of Microbiological Methods, 39, 23-31.
2.
Aggelis G., Iconomou, D., Christou, M., Bokas, D., Kotzailias, S., Christou, G., Tsagou, V. & Papanikolaou, S. (2003) Phenolic removal in a model olive oil mill wastewater using Pleurotus ostreatus in bioreactor cultures and biological evaluation of the process. Water Research, 37, 3897-3904.
3.
Aleksieva Z., Ivanova, D., Godjevargova, T. & Atanasov, B. (2002) A degradation of some phenol derivatives by Trichosporon cutaneum R57. Process Biochemistry, 37, 1215-1219.
4.
Bollman A. & Laanbroek, H.J. (2001) Continuous culture enrichment of ammonia-oxidizing bacteria at low ammonium concentration. FEMS Microbiology Ecology, 37, 211-221.
5.
Campos J.M., Fernandez, J.M.G., Mendez, R. & Lema, J.M. (1999) Nitrification at high ammonia loading rates in an activated sludge unit. Bioresource Technology, 68, 141-148.
6.
Carrera J., Baeza, J.A., Vicent, T. & Lafuente, J. (2003) Biological nitrogen removal of high-strength ammonium industrial wastewater with two-sludge system. Water Research , 37, 4211-4221.
7.
Fialova A., Boschke, E. & Bley, T. (2004) Rapid monitoring of the biodegradation of phenol-like compounds by the yeast Candida maltosa using BOD measurements. International Biodeterioration and Biodegradation, 54, 69-76.
8.
Gonzalez G., Herrera, G., Garcia, G.M.T. & Pena, M. (2001) Biodegradation of phenols in a continous process: comparative study of stirred tank and fluidized-bed bioreactors. Bioresource Technology, 76, 245-251.
9.
Greenbery, A.E., Trussell, R.R. & Clesceri, L.S. (1985) Standard Methods for the Examination of Water and Wastewater. American Public Health Association, 16th edition. American Public Health Association , New York.
10.
Grommen R., Hauteghem, I.V., Wambeke, M.V. & Verstraete, W. (2002) An improved nitrifying enrichment to remove ammonium and nitrite from fresh water aquaria systems. Aquaculture, 211, 115-124.
11.
Jiang Q.Q. & Bakken, L.R. (1999) Comparison of Nitrosospira strains isolated from terrestrial Environments. FEMS Microbiology Ecology, 30, 171-186.
12.
Kuai L. & Verstraete, W. (1998) Ammonium removal by the oxygen-limited autotrophic nitrification-denitrification system. Applied and Environmental Microbiology, 64, 4500-4506.
13.
Ordaz N.R., Lagunez, C.R. & Mayer, J.G. (2001) Phenol biodegradation using a repeated batch culture of Candida tropicalis in a multistage bubble column. Revista Latinoamericana de Microbiologia, 43, 19-25.
14.
Quintana M.G., Didion, C. & Dalton, H. (1997) Colorimetric method for a rapid detection of oxygenated aromatic biotransformation products. Biotechnology Technique , 11, 585-587.
15.
Rostron W.M., Stuckey, D.C. & Young, A.A. (2001) Nitrification of high strength ammonium wastewaters: comparative study of immobilization media. Water Research , 35, 1169-1175.
16.
Ruiz G., Jeison, D. & Chamya, R. (2003) Nitrification with high nitrite accumulation for the treatment of wastewater with high ammonia concentration. Water Research, 37, 1371-1377.
17.
Shiskowaski D.M. & Mavinic, D.S. (1998) Biological treatment of a high ammonia leachate: influence of external carbon during initial star-tup. Water Research, 32, 2533-2541.
18.
Vallini G., Frassinetti, S., Andrea, F.D., Catelani, G. & Agnolucci, M. (2001) Biodegradation of 4-(1-nonyl)phenol by axenic cultures of the yeast Candida aquaetextoris: identification of microbial breakdown products and proposal of a possible metabolic pathway. International Biodeterioration and Biodegradation, 47, 133-140.
19.
Watanabe K., Teramoto, M., Futamata, H. & Harayama, S. (1998) Molecular detection, isolation, and physiological characterization of functionally dominant phenol-degrading bacteria in activated sludge. Applied and Environmental Microbiology, 64, 4396-4402.
20.
Watson, S.W., Bock, E., Harms, H., Koops, H.P. & Hooper, A.B. (1989) Ammonia-oxidizing bacteria. In: Staley, J.T., Bryant, M.P., Pfennig, N. & Holt, J.G.Bergey's Manual of Systematic Bacteriology, Vol. 3, pp. 1818-1833. Williams and Wilkins, Baltimore, MD.
21.
Wett B. & Rauch, W. (2003) The role of inorganic carbon limitation in biological nitrogen removal of extremely ammonia concentrated wastewater. Water Research, 37, 1100-1110.
22.
Wong-Chong G.M. (1994). Retrofitting LTV coke plant wastewater treatment system to comply with preatment discharge limits. Iron Steel Engineering, 71, 26-28.
23.
Yamagishi T., Leite, J., Ueda, S., Yamaguchi, F. & Suwa, Y. (2001) Simultaneous removal of phenol and ammonia by an activated sludge process with cross flow filtration. Water Research, 35, 3089- 3096.
