Effect of Lyophilized Urine of Acute Rejected Kidney Transplant Recipients on Mp48 Production by Mesangial Cells in Culture

Abstract

MP48 is a protein expressed only in stimulated mesangials cells. This protein is expressed in diseased but not in normal kidney sections. It is also expressed in the liver and pancreas but not in the erythrocytes of healthy persons, whereas it is expressed in the erythrocytes of renal transplant recipients. This is the first study of the effect of lyophilized urine on MP48. The study showed that urine samples of acute rejected renal transplant recipients stimulated the cultured mesangial cells to produce MP48 protein, while the urine specimens of twelve normal persons did not. The immunological changes correlated with the acute renal rejection could lead to autocrine and apocrine secretion of IL-1 and other growth factors and their secretion in urine. Therefore, this experiment could be used to detect the effect of lyophilized urine specimens of acute rejected renal transplant recipients on cultured mesangial cells for production of MP48.