Flow cytometry (FCM) enables ploidy and proliferative tissue activity (PHASE S) to be quickly and objectively measured. This method is of predictive value regarding the biological behaviour of bladder tumours. The authors used FCM in a 5-year-follow-up study of 75 patients with papillary bladder carcinoma, who underwent therapeutic or staging transurethral resection in 1989. Results show a strict correlation between stage-ploidy-Progression as well as the significance of S-PHASE cells in relation to low-grade diploid tumours.
MobilioG., FranzolinN., Tinazzi-MartiniP., BernardiF., ZattiM.: L'indagine citogenetica come indice prognostico nei carcinomi superficiali della vescica. Urol., 4: 673, 1983.
2.
BertinoB., KnapeW.A., PytlinskaM., StraussK., HammouJ.C.: A comparative study of DNA content as measured by flow cytometry and image analysis in 1864 specimens. Anal. Cell. Pathol., 6 (4): 377–394, 1994.
3.
RuaS., CominoA., FrutteroA.: Utilità e limiti dell'analisi mediante citometria a flusso del contenuto in DNA delle cellule neoplastiche. Pathologica, 83: 421–439, 1991.
4.
FrankfurtO.S., SlocumH.K., RustumY.M., ArbuckS.G., PavelicZ.P., PetrelliN., HubenR.P., PontesE.J., GrecoW.R.: Flow cytometric analysis of DNA aneuploidy in primary and metastatic human solid tumors. Cytometry, 5: 71–80, 1984.
5.
GiarettiW.: Ploidy and proliferation evaluated by flow cytometry. An overview of techniques and impact in oncology. Tumori, 77: 403–419, 1991.
6.
HedleyD.W., FriedlanderM.L., TaylorJ.W.: Application of DNA flow cytometry to paraffin-embedded archival material for study of aneuploidy and its clinical significance; Cytometry, 6: 327–333, 1985.
7.
SaracinoG.A., DitonnoP., DisabatoG., TraficanteA., BattagliaM., LuciveroG., SelvaggiF.P.: Prediction of recurrence and progression in primary superficial bladder cancer with DNA flow cytometry. Eur, Urol., 21 (suppl. 1): 26–30, 1992.
8.
GiellaI.G., RingK., OlssonC.A., KarpF.S., BensonM.C.: The predictive value of flow cytometry and urinary cytology in the follow-up of patients with transitional cell carcinoma of the bladder. J. Urol., 148: 293–296, 1992.
9.
FranzolinN., AzzolinaL.S., BianchiG.P., SchiavoneD., BeltramiP., MobilioG.: Analisi citofluorimetrica del DNA nel tumore superficiale della vescica: correlazione tra fase S e prognosi. Acta Urol. Ital., IV (1): 123–125, 1989.
10.
TribukatiB.: Diagnostic and prognostic significance of modal DNA-values and proportion of S-phase cells in human carcinoma of the bladder. Quantitative image analysis in cancer cytology and histology. Mary J.Y., Rigant J.P. Eds. Amsterdam Elsevier, 315, 1986.
11.
Di SilverioF., von HelandM., De BerardinisE., IzziR., BuscariniM., De VitaR., ForteR., SeccarecciaF., MenottiA.: Prognostic role of flow cytometry in superficial bladder cancer. Eur. Urol., 21 (suppl. 1): 22–25, 1992.
12.
HosakaY.: DNA ploidy pattern of flow cytometry as indicator of malignancy and prognosis in bladder cancer. Nippon Rinsho, 50 (10): 2426–2431, 1992.
13.
BlonyousC.E., SchipperN.W., BaakJ.P., VosW., de WoogtH.J., MeyerC.J.: The value of morphometry and DNA flow cytometry in addition to classic prognosticators in superficial urinary bladder carcinoma. Am. J. Clin. Pathol., 91: 243–248, 1989.
14.
TribukatiB., GustafsonM., EspostiP.L.: The significance of ploidy and proliferation in the clinical and biological evaluation of bladder tumors: a study of 100 untreated cases. Br. J. Urol., 54: 130, 1982.
15.
BlomjousC.E.M., SchipperN.W., BaakJ.P.A., Van GalenE.M., De WoogtH.J., MeiyerC.J.L.M.: Retrospective study of prognostic importance of DNA flow cytometry of urinary bladder carcinoma. J. Clin. Pathol., 41: 21–25, 1988.
16.
TachibanaM., DeguchiN., BabaS., JitsukawaS., HataM., TazakiH.: Multivariate analysis of flow cytometric deoxyribonucleic acid parameters and histological features for prognosis of bladder cancer patients. J. Urol., 146: 1530–1534, 1991.
