Erythrothrombocytapheresis and Plasmathrombocytapheresis with Storage in T-sol of Platelets Collected by the New Amicus Cell Separator

Abstract

The Amicus cell separator is the latest apparatus introduced into the international market for high-yield, low WBC contamination and short-procedure time thrombocytapheresis. In its original configuration the apparatus collects platelets for subsequent resuspension in plasma and no collection of PRBC can be carried out along with thrombocytapheresis. In this paper we present the results of plasma-thrombocytapheresis and erythro-thrombocytapheresis after the adaptation of the Amicus to the collection and storage of platelets in a non-plasma medium and the concurrent collection of PRBC. From our study it is concluded that PRBC collection doesn't modify the quality of the platelet product obtained from random donors (platelets pre-count 263×10e3/μl) in terms of yield which is 4.6×10e11 platelets, WBC contamination (1.3×10e5) or procedure time (63±26 min.). The quality of the platelet products is satisfactory too, as measured by aggregation induced by collagen and ristocetin or by the substantial stability of membrane glycoproteins (CD62 - 63 - 51 - 36 - 42B). The concentrate had an average content of 58.8 g of hemoglobin per bag, a final volume of 376 ± 13 ml, (after the addition of 100 ml of SAG-M) and a normal mechanic and osmotic fragility.