Polymeric biomaterials: influence of phosphorylcholine polar groups on protein adsorption and complement activation

Abstract

The introduction to polymeric biomaterials of phosphorylcholine polar groups represents an approach towards the development of materials with improved blood compatibility. In this respect, two biomaterials, one a copolymer of butyl methacrylate and 2-methacryloyloxyethylphosphorylcholine (MPC), (poly(BMA-co-MPC) and the other, MPC-grafted Cuprophan, were examined with respect to their influence on protein adsorption and complement activation. Protein adsorption was studied by measurement of the adsorption of radiolabelled single proteins (albumin and fibrinogen), while complement activation was measured using radioimmunoassay for C3a des Arg. The investigation demonstrated that the polymers containing phosphorylcholine polar groups can achieve a marked reduction in protein adsorption and complement activation and supports the utilization of phosphorylcholine polar groups as a means of improving the compatibility of biomaterials for blood-contacting applications.

Keywords

Biomaterials Biomembrane-mimetic surfaces Phosphorylcholine Protein adsorption Complement activation

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References

Gilchrist

, Courtney

J.M.

. The design of biocompatible polymers. In: Ariëns

E.J.

, ed. Drug design. New York, USA: Academic Press, 1980; 251–75.

Fougnot

, Labarre

, Jozefonwicz

, Jozefowicz

. Modification of polymer surfaces to improve blood compatibility. In: Hastings

G.W.

, Ducheyne

, eds. Macromolecular biomaterials. Boca Raton, Florida, USA: CRC Press, 1984; 215–38.

Forbes

C.D.

, Courtney

J.M.

. Thrombosis and artificial surfaces. In: Bloom AL, Forbes CD, Thomas DP, Tuddenham EGD, eds. Haemostasis and thrombosis, 3rd edn. Edinburgh, Scotland: Churchill Livingstone, 1994; 2: 1301–24.

Engbers

G.H.

, Feijen

. Current techniques to improve the blood compatibility of biomaterial surfaces. Int J Artif Organs 1991; 4: 199–215.

Courtney

J.M.

, Yu

, Sundaram

. Immobilisation of macromolecules for obtaining biocompatible surfaces: In: Sleytr

U.B.

, Messner

, Pum

, Sara

, eds. Immobilised macromolecules. London: Springer-Verlag Limited, 1993; 175–93.

Durrani

A.A.

, Chapman

. Modification of polymer surfaces for biomedical applications. In: Feast

W.J.

, Munro

H.S.

, eds. Polymer surfaces and interfaces. New York, USA: John Wiley & Sons, 1987; 189–200.

Chapman

, Charles

S.A.

. A coat of many lipids in the clinic. Chemistry in Britain, 1992; 3: 253–6.

Brash

J.L.

. Protein adsorption and blood interaction. In: Szycher

, ed. Biocompatible polymers, metals, and composites. Lancaster, Pennsylvania, USA: Technomic, 1983; 35–52.

Brash

J.L.

. Role of plasma protein adsorption in the response of blood to foreign surfaces. In: Sharma

C.P.

, Szycher

, eds. Blood compatible materials and devices. Pennsylvania, USA: Technomic, 1991; 3–24.

10.

Szycher

. Thrombosis, hemostasis, and thrombolysis at prosthetic interfaces. In: Szycher

, ed. Biocompatible polymers, metals and composites. Lancaster, Pennsylvania, USA: Technomic, 1983; 1–33.

11.

Klinkmann

, Falkenhagen

, Courtney

J.M.

. Biomaterials and biocompatibility in hemodialysis. Contributions to Nephrology 1987; 55: 231–49.

12.

Ishihara

, Ueda

, Nakabayashi

. Preparation of phospholipid polymers and their properties as polymer hydrogel membranes. Polym J 1990; 22: 355–60.

13.

Ishihara

, Nakabayashi

, Fukumoto

, Aoki

. Improvement of blood compatibility on cellulose dialysis membrane: I. Grafting of 2-methacryloyloxyethyl phosphorylcholine on to a cellulose membrane surface. Biomaterials 1992; 13: 145–9.

14.

. Modification of polymeric biomaterials for improved blood compatibility: Investigation of protein adsorption and in vitro blood response. Glasgow: University of Strathclyde, UK. 1993; 134 Thesis.

15.

Ishihara

, Aragaki

, Ueda

, Watenabe

, Nagakabayashi

. Reduced thrombogenicity of polymers having phospholipid polar groups. J Biomed Mater Res 1990; 24: 1069–77.

16.

Coutts

A.G.

. Investigation of protein-biomaterial interactions. Glasgow: University of Strathclyde, UK 1993; 66 Thesis.

17.

Ishihara

, Takayama

, Nakabayashi

. Improvement of blood compatibility on cellulose dialysis membrane: II. Blood compatibility of phospholipid polymer grafted cellulose membrane. Biomaterials 1992; 13: 235–9.

18.

Ishihara

, Ziats

N.P.

, Tierney

B.P.

, Nakabayashi

, Anderson

J.M.

. Protein adsorption from human plasma is reduced on phospholipid polymers. J Biomed Mater Res 1991; 24: 1397–1407.