In this study we will demonstrate that LAK cells, in vitro, can lyse hematologic neoplastic cells with a minor toxicity of the staminal autologous marrow cells. In fact, after bone marrow and LAK co-culture at a ratio of 1/1 for 8 hours, the inhibition on the GEMM colonies resulted to be 20% less compared to the untreated marrow. These data make LAK an inviting agent for marrow purging in autologous bone marrow transplantation.
GorinN.C.Autologous bone marrow transplantation for acute myelocitic leukemia in first remission: a european survey of the role of marrow purging.Blood1990; 8: 1606–14.
2.
RosembergS.A., LotzeM.T., MuulL.M.Observation on the systemic administration of autologous lymphokine activated killer cells and recombinant interleukin-2 to patients with metastatic cancer.New Engl J Med1985; 1485–92.
3.
MuulL.M., DirectorE.P., HjattC.L., RosembergS.A.Large scale production of human lymphokine activated killer cells for use in adoptive immunotherapy.J of Immunological Method1986; 88: 265–75.
4.
ParmianiG., RivoltiniL., Gambacorta-PasseriniC.Purging di midollo osseo con cellule LAK. Atti 2° Simposio Nazionale sulla Criopreservazione delle cellule emopoietiche.MorettiL., SparaventiG., LucarelliG., eds. Pesaro1990; 130–5.
5.
MorettiL., PazzagliaC., SparaventiG., AnnibaliM.Criopreservazione di cellule linfoidi, linee cellulari e LAK.Atti 2° Simp Naz sulla Criopreservazione delle cellule emopoietiche.MorettiL., SparaventiG., LucarelliG., eds. Pesaro1990; 17–22.
6.
GiuliodoriL., AnnibaliM., Baldi. Criopreservazione di cellule LAK per il purging di midollo osseo.Tumori1991; 173.
7.
FfindleiH.W., NageedA., NasrS., AbdelsalamH.Recombinant interleukin-2 activated periteral blood lymphocites from children with acute leukemia to kill autologous leukemic cells.Cancer1988; 62: 1928–31.
