One of the drawbacks of solid phase enzyme reactors is the sharp decrease in substrate affinity as shown by an increase in the Km app value. To circumvent this problem a liquid phase enzyme reactor system was designed. Biotin labeled L-Tryptophan side chain oxidase (TSO) was directly injected into the plasma circuit of a filter plasmaphoresis system of rabbits and adsorbed onto an Avidin column prior to its reentry into the animal's general blood circulation. In five experiments total L-Tryptophan depletion could be observed in the plasma circuit during 60 minutes of the experiment with complete readsorption of the enzyme to the Avidin column.
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DennisM.B., SchmerG., RastelliL.N.Successful long term use of a plasmaphoresis reactor system in dogs with lymphoma.Trans ASAIO1983; 29: 744–8.
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11.
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