Exposure of human neutrophils to a conventional, acidic, lactate-containing peritoneal dialysis solution (PDS) resulted in the development of a prompt and substantial intracellular acidosis. It is possible that this intracellular acidosis contributes to cellular dysfunction.
VeechR.L.The untoward effects of the anions of dialysis fluids.Kidney Int1988; 34: 587–97.
4.
IngB.L., GuptaD.K., NawabZ.M., ZhouF.Q., RahmanM.A., DaugirdasJ.T.Suppression of neutrophil superoxide production by conventional peritoneal dialysis solution.Int J Artif Organs1988; 11: 351–4.
5.
ManahanF.J., IngB.L., ChanJ.C.Effects of bicarbonte-containing versus lactate-containing peritoneal dialysis solutions on superoxide production by human neutrophils.Artif Organs1989; 13: 495–7.
6.
GuptaD.K., IngB.L., ManahanF.J.Superoxide generation by neutrophils after exposure to a conventional peritoneal dialysis solution for different time periods.Int J Artif Organs1990; 13: 228–30.
7.
DuweA.K., VasS.I., WeatherheadJ.W.Effect of the composition of peritoneal dialysis fluid on chemiluminescence, phagocytosis and bactericidal activity in vitro.Infect Immun1981; 33: 130–5.
8.
HarveyD.M., SheppardK.J., MorganA.G., FletcherJ.Effect of dialysate fluids in phagocytosis and killing by normal neutrophils.J Clin Microbiol1987; 25: 1424–7.
9.
Van BronswijkH., VerbrughH.A., HeeziusECJM, Van der MeulenJ., OeP.L., VerhoefJ.Dialysis fluids and local host resistance in patients on continuous ambulatory peritoneal dialysis.Eur J Clin Microbiol Infect Dis1988; 7: 368–73.
10.
TopleyN., AlobaidiH.M.M., DaviesM., ColesG.A., WilliamsJ.D., LloydD.The effect of dialysate on peritoneal phagocyte oxidative metabolism.Kidney Int1988; 34: 404–11.
11.
Van BronswijkH., VerbrughH.A., BosH.J.Cytotoxic effects of commercial continuous ambulatory peritoneal dialysis (CAPD) fluids and bacterial exoproducts on human mesothelial cells in vitro.Perit Dial Int1989; 9: 197–202.
12.
SimchowitzL.Intracellular pH modulates the generation of superoxide radicals by human neutrophils.J Clin Invest1985; 76: 1079–89.
13.
ZhouX.J., YuA.W., IngT.S., VaziriN.D.Neutrophilic intracellular acidosis after exposure to peritoneal dialysis solution (PDS) containing lactate (Abstract).J Am Soc Nephrol1991; 2: 370.
14.
CurnutteJ.T., BabiorB.M.Biological defense mechanisms. The effect of bacteria and serum on superoxide production by granulocytes.J Clin Invest1974; 53: 1662–72.
15.
SalehA.M., BatlleD.C.Kinetic properties of the Na+H+ antiporter of lymphocytes from the spontaneously hypertensive rat: role of intracellular pH.J Clin Invest1990; 85: 1734–9.
16.
ThomasJ.A., BuchsbaumR.N., ZimniakA., RackerE.Intracellular pH measurements in Ehrlich ascites tumor cells utilizing spectrocopic probes generated in situ.Biochem1978; 18: 2210–8.
17.
ParadisoA.M., TsieuR.Y., MachenT.Na+-H+ exchange in gastric glands as measured with a cytoplasmic-trapped, fluorescent pH indicator.Proc Natl Acad Sci1984; 81: 7436–40.
18.
YuA., ZhouF., SongR., NawabZ., RahmanM., IngT.Peritoneal dialysis solution pH and lactate in the inhibition of neutrophilic superoxide formation (Abstract).J Am Soc Nephrol1990; 2: 393.
19.
De HemptinneA., MarrannesR., VanhellB.Influence of organic acids on intracellular pH.Am J Physiol1983; 245(Cell physiol 14): C178–83.
20.
DubinskyW.P., RacherE.The mechanism of lactate transport in human erythrocytes.J Membr Biol1978; 44: 25–36.
MooreA.L., GrantB.W., DorighiJ.A., BelinsonJ.L., StewartJ.A., AlbertiniR.J.Effect of commercial peritoneal dialysis fluids on the lytic function of lymphokine-activated killer cells.J Bio Response Mod1988; 7: 401–8.
