Hepatocytes encapsulated in an alginate-polylysine-membrane were used to lower bilirubin levels in hyperbilirubinemia. The animal model was the Gunn rat. The microencapsulated hepatocytes were implanted intraperitoneally. 15×166 microencapsulated hepatocytes from Wistar rats lowered the bilirubin from 14 mg/100 ml to 6 mg/100 ml after 20 days. 15×106 microencapsulated hepatocytes from Sprague-Dawley rats lowered the bilirubin levels from 8 mg/100 ml to 5 mg/100 ml. They were as effective as free Sprague-Dawley hepatocytes. 12×106 Guinea pig hepatocytes, whether encapsulated or free, lowered the bilirubin from 9.36 mg/100ml to 4.37 mg/100 ml.
ChangT.M.S., MacintoshF.C., MasonS.G.Semipermeable aqueous microcapsules: I Preparation and properties.Can J Physio Pharmacol1966; 44: 115–8.
4.
ChangT.M.S.Artificial cells. Monograph, Charles C, Thomas Publisher, Springfield, III1972; 207.
5.
LimS.Microencapsulated hepatocytes as a potential bioartificial liver.Proceedings of the Third Meeting of ISAO Supplemental1981; 5.
6.
WongH., ChangT.M.S.Bioartificial liver: implanted artificial cells microencapsulated living hepatocytes increase survival of liver failure rats.Int J Artif Organs1986; 9: 335–6.
7.
SunA.M., CaiZ., ShiZ., MaF., O'SheaG.M.Microencapsulated hepatocytes: an in vitro and in vivo study.Biomat Artif Cells Artif Organs1987; 15(2): 483–96.
8.
WongH., ChangT.M.S.The viability and regeneration of artificial cell microencapsulated rat hepatocytes xenograft transplants in mice.Biomat Artif Cells Artif Organs,1988; 16(4): 731–9.
9.
SeglenO.Preparation of isolated rat liver cells.Methods in Cell Biology1976; 13: 29–83.
10.
BruniS., ChangT.M.S.Hepatocytes immobilised by microencapsulion in artificial cells: effects on hyperbilirubinemia in Gunn rats.Biomat Artif Cells Artif Organs,1989; 17(4): 403–11.
11.
KamadaN.The immunology of experimental liver transplantation in the rat.Immunology1985; 55: 369–89.
12.
HussinD., GigouM., FrancoD., BismuthH.Specific transplantation tolerance induced by spontaneously tolerated liver allograft in inbred strains of rats.Transplantation1980; 29: 418–9.
13.
HussinD., CharpentierB., GugenheimJ.Spontaneous long term acceptance of RT-1 incompatible liver allograft in inbred rats.Transplantation1983; 36: 615–20.
