Stroma-free hemoglobin is prepared from human blood and then cross-linked with glutaraldehyde to produce soluble polyhemoglobin. Results from animal studies show that in the case of polyhemoglobin, its level in the circulation remained unchanged at 77% of the injected amount when followed for 3 hours; whereas stroma-free hemoglobin was removed rapidly with a half-life of 87 minutes. Thus, cross-linking stroma-free hemoglobin into the polyhemoglobin form has solved the major problem of rapid removal of stroma-free hemoglobin from the circulation.
ChangT.M.S., MacintoshF.C., MasonS.G.: Semipermeable aqueous microcapsules: Preparation and properties.Can J. Physiol. Pharmacol., 44, 115, 1966.
7.
ChangT.M.S.: Artificial Cells.Charles C. Thomas.Springfield, p. 93, 1972.
8.
ChangT.M.S.: Stabilizathion of enzymes by microencapsulation with a protein solution or by microencapsulation followed by cross-linking with glutaraldehyde.Biochem. Biophys. Res. Comm., 44(6), 1531, 1971.
9.
RabinerS.F., HelbertJ.R., LopasH., FriedmanL.H.: Evaluation of a stroma-free hemoglobin solution for use as a plasma expander.J. Exptl. Med., 126, 1127, 1967.
10.
MokW., ChenD.E., MazurA.: Cross-linked hemoglobins as potential plasma protein extenders.Fed. Proc., 34(6), 1458, 1975.
11.
ChangT.M.S.: Semipermeable aqueous microcapsules («artificial cells») with emphasis on experiments in an extracorporeal shunt system.Trans. Am. Soc. Artif. Intern. Organs, 12, 13, 1966.
12.
SloviterH.A.: Perfluoro compounds as artificial erythrocytes.Fed. Proc., 34(6), 1484, 1975.
13.
GeyerR.P.: «Bloodless» rats through the use of artificial blood substitutes.Fed. Proc., 34(6), 1499, 1975.
14.
NaitoR., YokoyamaK.: An improved perfluorodecalin emulsion. In: Blood Substitutes and Plasma Expanders (JamiesonG.A., and GreenwaltT.J., eds.) Alan R. Liss Inc., New York, 81, 1978.
