Immobilization and Characterization of L-Asparaginase on Hollow Fibers

Abstract

L-Asparaginase was immobilized with various methods: cyanogen bromide, cyanuric chloride and cross-linking with glutaraldehyde.

A dialysis device was transformed in a bioreactor with the enzyme immobilized on the outer surface of hollow fibers.

Results on kinetic behavior, stability in storage and lyophilization are reported.

Keywords

Detoxification Hollow fibers L-Asparaginase Leukemia Immobilized enzymes

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