Restricted accessResearch articleFirst published online 2026-3
Development of a three-dimensional collagen hydrogel model incorporating laurus nobilis extract–chitosan nanoparticles against ovarian cancer SKOV3 cells
The persistent challenges posed by ovarian cancer, marked by its high mortality rates and resistance to conventional therapies, drive a continuous search for innovative and effective treatment strategies. In the current study, laurus nobilis extract was loaded into chitosan nanoparticles to investigate its potential anti-cancer effects against SKOV3 ovarian cancer cells.
Methods:
The developed nanocarriers were loaded into a collagen hydrogel to better mimic the tumor microenvironment. In vitro studies including scanning electron microscopy, cell viability assay, cell migration assay, hemocompatibility assay, release assay, real-time polymerase chain reaction assay, cell imaging, and anti-inflammatory assays were performed in order to characterize the three-dimensional model.
Results:
The study showed that our developed system reduced the viability and migratory activity of cancer cells. Real-time polymerase chain reaction assay suggested that anti-cancer effects of our developed system may be attributed to the downregulation of Polo-Like Kinase 1 (PLK1) and Poly (ADP-Ribose) Polymerase 1 (PARP1) genes.
Conclusion:
Our developed model may be used for studying the effects of different anti-cancer drugs on ovarian cancer cells.
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