In recent years, a variety of new technologies have been proposed that allow rapid qualitative and quantitative microbiological analyses. In this paper we discuss the urgent needs for reliable and rapid microbiological analytical techniques in different applicative fields involving the research, production and medical application of implant materials, and the potential benefits derived from the use of new methods for rapid bacterial quantification. Current compendial methods are easy to perform and have gained confidence over their long period of use, but the supplemental use of new technologies could represent real breakthroughs whenever sensitive and rapid responses are urgently required and not met by the tests currently in use. Overall, the new microbiological methods require critical evaluation depending on their specific type of application and they may still not be thought of as totally substitutive, but they certainly exhibit considerable potential for different areas of biomaterials, as well as for advanced therapy medicinal and tissue engineering treatments.
ZaidiN., KonstantinouK., ZervosM.The role of molecular biology and nucleic Acid technology in the study of human infection and epidemiology. Arch Pathol Lab Med2003; 127: 1098–105.
2.
NobleR.T., WeisbergS.B.A review of technologies for rapid detection of bacteria in recreational waters. J Water Health2005; 3: 381–92.
3.
NewbyP., DalmasoG., LonardiS., RileyB., CooneyP., TyndallK.The introduction of Qualitative Rapid Microbiological Methods for Drug-Product Testing. Pharmaceutical Technology Process Analytical Technology2004; 6–12.
IqbalMS, and RashidF.Rapid Detection of Bacterial Growth by Infrared Spectrophotometry. Appl Spectrosc1991; 45: 1366–7.
6.
BoonkitticharoenV., EhrhardtJ.C., KirchnerP.T.Radiometric assay of bacterial growth: analysis of factors determining system performance and optimization of assay technique. J Nucl Med1987; 28: 209–17.
7.
GraciaE., FernandezA., ConchelloP., AlabartJ.L., PerezM., AmorenaB.In vitro development of Staphylococcus aureus biofilms using slime-producing variants and ATP-bioluminescence for automated bacterial quantification. Luminescence1999; 14: 23–31.
8.
CampocciaD., MontanaroL., AgheliH., SutherlandD.S., PiriniV., DonatiM.E., ArciolaC.R.Study of Staphylococcus aureus adhesion on a novel nanostructured surface by chemiluminometry. Int J Artif Organs2006; 29: 622–9.
9.
FeliceC.J., MadridR.E., OliveraJ.M., RotgerV.I., ValentinuzziM.E.Impedance microbiology: quantification of bacterial content in milk by means of capacitance growth curves. J Microbiol Methods1999; 35: 37–42.
10.
UrA., BrownD.F.Impedance monitoring of bacterial activity. J Med Microbiol1975; 8: 19–28.
11.
StevensC.M., SwaineD., ButlerC., CarrA.H., WeightmanA., CatchpoleC.R., HealingD.E., ElliottT.S.Development of o.a.s.i.s., a new automated blood culture system in which detection is based on measurement of bottle headspace pressure changes. J Clin Microbiol1994; 32: 1750–6.
12.
RipaK.T., MardhP.A., HoveliusB., LjungholmK.Microcalorimetry as a tool for evaluation of blood culture media. J Clin Microbiol1977; 5: 393–6.
13.
RellerL.B.Recent and innovative methods for detection of bacteremia and fungemia. Am J Med1983; 75: 26–30.
14.
PezzloM.T.Automated methods for detection of bacteriuria. Am J Med1983; 75: 71–8.
15.
LockettS.J., O'RandM., RinehartC.A., KaufmanD.G., HermanB., JacobsonK.Automated fluorescence image cytometry. DNA quantification and detection of chlamydial infections. Anal Quant Cytol Histol1991; 13: 27–44.
16.
FouchetP., JayatC., HechardY., RatinaudM.H., FrelatG.Recent advances of flow cytometry in fundamental and applied microbiology. Biol Cell1993; 78: 95–109.
17.
Van PouckeS.O., NelisH.J.A 210-min solid phase cytometry test for the enumeration of Escherichia coli in drinking water. J Appl Microbiol2000; 89: 390–6.
18.
MarianiB.D., LevineM.J., BoothR.E.Jr., TuanR.S.Development of a novel, rapid processing protocol for polymerase chain reaction-based detection of bacterial infections in synovial fluids. Mol Biotechnol1995; 4: 227–37.
19.
GriffaisR., ThibonM.Detection of Chlamydia trachomatis by the polymerase chain reaction. Res Microbiol1989; 140: 139–41.
20.
SchmidtM., HourfarM.K., NicolS.B., WahlA., HeckJ., WeisC., TonnT., SpenglerH.P., MontagT., SeifriedE., RothW.K.A comparison of three rapid bacterial detection methods under simulated real-life conditions. Transfusion2006; 46: 1367–73.
21.
KalogianniD.P., GouraS., AletrasA.J., ChristopoulosT.K., ChanosM.G., ChristofidouM., SkoutelisA., IoannouP.C., PanagiotopoulosE.Dry reagent dipstick test combined with 23S rRNA PCR for molecular diagnosis of bacterial infection in arthroplasty. Anal Biochem2007; 361: 169–75.
22.
WolffsP.F., GlencrossK., ThibaudeauR., GriffithsM.W.Direct quantitation and detection of salmonellae in biological samples without enrichment, using two-step filtration and real-time PCR. Appl Environ Microbiol2006; 72: 3896–900.
23.
PolliceM., YangH.L.Use of nonradioactive DNA probes for the detection of infectious bacteria. Clin Lab Med1985; 5: 463–73.
24.
LehtolaM.J., LoadesC.J., KeevilC.W.Advantages of peptide nucleic acid oligonucleotides for sensitive site directed 16S rRNA fluorescence in situ hybridization (FISH) detection of Campylobacter jejuni, Campylobacter coli and Campylobacter lari. J Microbiol Methods2005; 62: 211–9.
25.
Parenteral Drug Association.PDA Technical Report No. 33, Evaluation, Validation and Implementation of New Microbiological Testing Methods. May 2000.
26.
International Organization for Standardization.Sterilization of medical devices - estimation of the population of microorganisms on product. Part 2: Guidance. EN ISO 1174-2. 1996.
27.
United States Pharmacopeia.Validation of Alternative Microbiological Methods. USP <1223>. 2006. p 3807–10.
28.
European Pharmacopoeia. Alternative methods for control of microbiological quality. Ph Eur <5.1.6>. General texts on Sterility <5.1>. 2006. p 4131–42.
29.
ArciolaC.R., AlviF.I., AnY.H., CampocciaD., MontanaroL.Implant infection and infection resistant materials: a mini review. Int J Artif Organs2005; 28: 1119–25.
30.
TrampuzA., OsmonD.R., HanssenA.D., SteckelbergJ.M., PatelR.Molecular and antibiofilm approaches to prosthetic joint infection. Clin Orthop Rel Res2003; 414: 69–88.
31.
TunneyM.M., PatrickS., GormanS.P., NixonJ.R., AndersonN., DavisR.I., HannaD., RamageG.Improved detection of infection in hip replacements. A currently underestimated problem. J Bone Joint Surg (Br)1998; 80: 568–72.
32.
NeutD., van HornJ.R., van KootenT.G., van der MeiH.C., BusscherH.J.Detection of biomaterial-associated infections in orthopaedic joint implants. Clin Orthop Rel Res2003; 413: 261–8.
33.
LamariF.N., AnastassiouE.D., KolonitsiouF., DimitracopoulosG., KaramanosN.K.Potential use of solid phase immunoassays in the diagnosis of coagulase-negative staphylococcal infections. J Pharm Biomed Anal2004; 34: 803–10.
34.
MolanderA., LundquistP., PapapanouP.N., DahlenG., ReitC.A protocol for polymerase chain reaction detection of Enterococcus faecalis and Enterococcus faecium from the root canal. Int Endod J2002; 35: 1–6.
35.
DempseyK.E., RiggioM.P., LennonA., HannahV.E., RamageG., AllanD., BaggJ.Identification of bacteria on the surface of clinically infected and non-infected prosthetic hip joints removed during revision arthroplasties by 16S rRNA gene sequencing and by microbiological culture. Arthritis Res Ther2007; 9: R46.
36.
ForrestG.N., MehtaS., WeekesE., LincalisD.P., JohnsonJ.K., VeneziaR.A.Impact of rapid in situ hybridization testing on coagulase-negative staphylococci positive blood cultures. J Antimicrob Chemother2006; 58: 154–8.
37.
StenderH.PNA FISH: an intelligent stain for rapid diagnosis of infectious diseases. Expert Rev Mol Diagn2003; 3: 649–55.
38.
KatsikogianniM., MissirlisY.F.Concise review of mechanisms of bacterial adhesion to biomaterials and of techniques used in estimating bacteria-material interactions. Eur Cell Mater2004; 8: 37–57.
39.
MontanaroL., CampocciaD., RizziS., DonatiM.E., BreschiL., PratiC., ArciolaC.R.Evaluation of bacterial adhesion of Streptococcus mutans on dental restorative materials. Biomaterials2004; 25: 4457–63.
40.
HarrisL.G., FosterS.J., RichardsR.G.An introduction to Staphylococcus aureus, and techniques for identifying and quantifying S. aureus adhesins in relation to adhesion to biomaterials: review. Eur Cell Mater2002; 4: 39–60.
41.
ArciolaC.R., BustanjiY., ContiM., CampocciaD., BaldassarriL., SamoriB., MontanaroL.Staphylococcus epidermidis-fibronectin binding and its inhibition by heparin. Biomaterials2003; 24: 3013–9.
42.
ASTM International.Standard Test Method For Determining the Activity of Incorporated Antimicrobial Agent(s) in Polymeric or Hydrophobic Materials. ASTM D 2180-01.
43.
ASTM International.Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Un-der Dynamic Contact Conditions. ASTM E2149-01.
44.
International Organization for Standardization.Textile fabrics - Determination of antibacterial activity - Agar diffusion plate test. EN ISO 20645: 2004.
45.
International Organization for Standardization.Textiles - Determination of antibacterial activity of antibacterial finished products. ISO 20743: 2007.
46.
Japanese Standards Association. Antimicrobial products-test for antimicrobial activity and efficacy. Japanese Industrial Standard (JIS) Z2801. 2000.
47.
American Association of Textile Chemists and Colorists. Antibacterial finishes on textile materials: an assessment.Test method 100-1993.AATCC technical manual. p 143–4.
48.
Antibacterial finishes on textile materials: parallel streak method. Test method 147-1993. AATCC technical manual. p 253–4.
49.
International Organization for Standardization.Sterilization of medical devices - Microbiological methods - Part 1: Estimation of population of microorganisms on products. EN ISO 11737-1: 1995.
50.
International Organization for Standardization.Sterilization of medical devices - Microbiological methods - Part 2: Tests of sterility performed in the validation of a sterilization process. EN ISO 11737-2: 1998.
51.
International Organization for Standardization. Cleanrooms and associated controls environments - Biocontamination control. EN ISO 14698: 2003.
52.
European Pharmacopoeia. Sterility test. Ph Eur <2.6.1>.
53.
United States Pharmacopeia. Sterilization and Sterility Assurance of Compendial Articles. USP <1211>. 2006.
54.
United States Pharmacopeia.Microbiological Evaluation of Cleanrooms and Other Controlled Environments. USP <1116>. 2006.
55.
United States Pharmacopeia. Sterility tests. USP <71>. 2006.
