Cross-linked urethane/urea membranes with two soft segments were prepared by extending a poly(propylene oxide) based tri-isocyanate-terminated prepolymer (PUR) with polybutadiene diol (PBDO). The ratio of prepolymer and polybutadiene diol was varied to yield cross-linked membranes with different compositions, exhibiting different degrees of phase-separation of the PBDO segments in the bulk and of surface enrichment in PUR. In this work, surface energy and hemocompatibility aspects (hemolysis and thrombosis) of the PUR/PBDO membranes were evaluated. The results showed that the membrane surface energy increased with the PBDO content until 25% of PBDO, and decreased thereafter. The introduction of the second, more hydrophobic, soft segment (PBDO) in the PUR membranes turned hemolytic into non-hemolytic membranes and, for a blood-material contact time of 10 minutes, decreased the thrombogenicity significantly. The 10% PBDO membrane was the least thrombogenic and was also non-hemolytic. The hemolysis degree did not vary significantly with the PBDO content while, for blood-material contact times of 10 minutes, the thrombogenicity increased with an increase in PBDO content above 10%. Membrane thrombogenicity varied with the blood-material contact time. For blood contact times of 10 minutes, all membranes tested were less thrombogenic than glass.
ParkJ.B., LakesR.S.Biomaterials. An introduction.2nd Edition. New York: Plenum Press; 1992. p 280–91.
2.
RechaviaE., LitvackF., FishbienM.C., NakamuraM., EiglerN.Biocompatibility of polyurethane-coated stents: tissues and vascular aspects. Cathet Cardiovasc Diagn1998; 45: 202–7.
3.
BurkeJ.F., DidisheimP., GoupilD., HellerJ., KaneJ.B., KatzJ.L., KimW.S., LemonJ.E., RefojoM.F., RobbleeL.S., SmithD.C., SweeneyJ.D., TompkinsR.G., WatsonJ.T., YagerP., YarmushM.L.Application of materials in medicine and dentistry. In: RatnerB.D., HoffmanA.S., SchoenF.J., LemonsJ.E., eds. Biomaterials science. An introduction to materials in medicine.New York: Academic Press; 1999. p 263–368.
4.
WatanabeH., HayashiJ., OhzekiH., MoroH., SugawaraM., EguchiS.Biocompatibility of a silicone-coated polypropylene hollow fibre oxygenator in an in vitro model. Ann Thorac Surg1999; 67: 1315–9.
5.
SegersP.A., HeidaJ.F., de VriesI., MaasC., BoogaartA.J., EilanderS.Clinical evaluation of nine hollow fibre oxygenators. Perfusion2001; 16: 95–106.
6.
NogawaA.The future of artificial lungs: an industry perspective. J Artif Organs2002; 5: 211–5.
7.
WickramasingheS.R., GarciaJ.D., HanB.Mass and momentum transfer in hollow fibre blood oxygenators. J Memb Sci2002; 208: 247–56.
8.
HaworthW.S.The development of the modern oxygenator. Ann Thorac Surg2003; 76: S2216–9.
JohnellM., ElgueG., LarsonA., StefanT., SuegbhnA.Coagulation, fibrinolysis, and cell activation in patients and shed mediastinal blood during coronary artery bypass grafting with a new heparin-coated surface. J Thorac Cardiovasc Surg2002; 124: 321–32.
11.
SegesserL.K.Safety and efficacy of heparin-bonded surfaces in cardiopulmonary bypass. J Thorac Cardiovasc Surg2003; 125: S90–1.
12.
WahbaA., PhilippA., BehrR., BirnbaumD.E.Heparin-coated equipment reduces the risk of oxygenators failure. Ann Thorac Surg1998; 65: 1310–2.
13.
LevyM., HartmanA.R.Heparin-coated bypass circuits in cardiopulmonary bypass: improved biocompatibility or not. Int J Cardiol1996; 53(Suppl 1): S81–7.
14.
WendelH.P., ZiemerG.Coating-techniques to improve the hemocompatibility of artificial devices used for extracorporeal circulation. Eur J Cardiothorac Surg1999; 16: 342–50.
15.
JanvierG., BaqueyC., RothC., BenillanN., BélisleS., HardyJ.F.Extracorporeal circulation, hemocompatibility, and biomaterials. Ann Thorac Surg1996; 62: 1926–34.
16.
BamfordC.H., Al-LameeG.Studies in polymer surface modification and grafting for medical uses: 2. Application to arterial blood filters and oxygenators. Polymer1996; 37: 4885–9.
17.
AndersonJ.M., GristinaG., HansonS.R., HarkerL.A., JohnsonR.J., MerrittK., NaylorP.T., SchoenF.J.Host reactions to biomaterials and their evaluation. In: RatnerB.D., HoffmanA.S., SchoenF.J., LemonsJ.E., eds. Biomaterials science. An introduction to materials in medicine.New York: Academic Press; 1999. p 165–214.
DeppischR., StorrM., BuckR., GöhlH.Blood material interactions at the surfaces of membranes in medical applications. Separation and Purification Technology1998; 14: 241–54.
ChenK.Y., KuoJ.F., ChenC.Y.Synthesis, characterization and platelet adhesion studies of novel ion-containing aliphatic polyurethanes. Biomaterials2000; 21: 161–71.
24.
LinD.T., YuongT.H., FangY.Studies on the effect of surface properties on the biocompatibility of polyurethanes membranes. Biomaterials2001; 22: 1521–9.
25.
SpijkerH.T., GraaffR., BoonstraP.W., BusscherH.J., van OeverenW.On the influence of flow conditions and the wettability on blood material interactions. Biomaterials2003; 24: 4717–27.
26.
WilsonD.J., RhodesN.P., WilliamsR.L.Surface modification of a segmented poly(ether)urethane using a low-powered gas plasma and its influence on the activation of the coagulation system. Biomaterials2003; 24: 5069–81.
27.
PoussardL., BurelF., CouvercelleJ.P., MerhiY., TabrizianM., BunelC.Hemocompatibility of new ionic polyurethanes: influence of carboxylic group insertion modes. Biomaterials2004; 25: 3473–83.
28.
ZhaoC-T, PinhoM.N.Design of polypropylene oxide/polybutadiene bi-soft segment urethane/urea polymer for pervaporation membranes. Polymer1999; 40: 6089–97.
29.
QueirozD.P., GonçalvesM.C., PinhoM.N.Tailoring of phase segregation structures in bi-soft segment urethane/urea polymer membranes. J Appl Polym Sci (In press).
30.
QueirozD.P., do RegoA.M.B., de PinhoM.N.Bi-soft segment polyurethane membranes: surface studies by X-ray photoelectron spectroscopy. J Membr Sci2006; 281: 239–44.
31.
Ferreira MarquesM.F., GordoP.M., GilC.L., de LimaA.P., QueirozD.P., PinhoM.N., KajcsosZ.S.Gas permeability and temperature-dependent free-volume studies in polyurethane membranes by positron lifetime and Doppler spectroscopy. Materials Science Forum2004; 445-446: 289–91.
32.
International Standards Organization (ISO).ISO 10993-4: 2002 – Biological evaluation of medical devices. Part 4: Selection of tests for interactions with blood. 2nd Edn. Geneva: ISO; 2002.
33.
RusanovA.I., ProkhorovV.A.Interfacial tensiometry. Vol. 3. Amsterdam: Elsevier; 1996. p 82–179.
34.
OwensD.K., WendtR.C.Estimation of the surface of free energy of polymers. J Appl Polym Sci1969; 13: 1741–7.
35.
RabelW.Einige Aspekte der Benetzungstheorie und ihre Anwendung auf die Untersuchung und Veränderung der Oberflächeneigenschaften von Polymeren. Farbe Lack1971; 77: 997–1006.
36.
KaelbleD.H.Dispersion-polar surface tension properties of organic solids. Journal of Adhesion1970; 2: 66–88.
37.
KaelbleD.H.Physical chemistry of adhesion.New York: John Wiley and Sons; 1971.
38.
American Society for Testing of Materials (ASTM).ASTM F 756-00 – Standard practice for assessment of hemolytic properties of materials.West Conshohocken, PA, USA: ASTM; 2000.
39.
ImaiY., NoséY.A new method for evaluation of antithrombogenicity. J Biomed Mater Res1972; 6: 165–72.
40.
AllmérK., HilbornP.H., HultA., RånbyB.Surface modification of polymers. V. Biomaterial applications. J Polym Sci [A1]1990; 28: 173–83.
ZwartA., van AssendelftO.W., BullB.S., EnglandJ.M., LewisS.M., ZijlstraW.G.Recommendations for reference method for haemoglobinometry in human blood (ICSH standard 1995) and specifications for international haemoglobincyanide standard (4th ed). J Clin Pathol1996; 49: 271–4.
43.
Van AssendelftO.W., BuursmaA., ZijlstraW.G.Stability of haemoglobincyanide standards. J Clin Pathol1996; 49: 275–7.
44.
MooreG.L., LedfordM.E., MerydithA.A micromodification of the Drabkin assay for measuring plasma hemoglobin in the range of 5 to 2000 mg/dL. Biochem Med1981; 26: 167–73.
45.
NojimaK., SanuiK., OgataN., YuiN., KataokaK., SakuraiY.Material characterization of segmented polyether poly(urethane-ures-amide)s and its implication in blood compatibility. Polymer1987; 28: 1017–24.
46.
YiuN., TanakaJ., SanuiK., OgataN., KataokaK., OkanoT., SakuraiY.Characterization of the microstructure of poly(propylene oxide) segmented polyamide and its suppression of platelet adhesion. Polym J1984; 16: 119–28.
47.
KajiyamaT., TakaharaA.Surface properties and platelet reactivity of segmented poly(etherurethanes) and poly(etherurethaneureas). J Biomater Appl1991; 6: 42–71.
