Restricted accessResearch articleFirst published online 2003-6
In Vitro Behaviour of Osteoblasts Cultured on Orthopaedic Biomaterials with Different Surface Roughness,Uncoated and Fluorohydroxyapatite-Coated,Relative to the in Vivo Osteointegration Rate
The effects of two surfaces with different roughness (Low Roughness, LR: Ra: 5.6–5.9 μm; High Roughness, HR: Ra: 21.5–22.5 μm), uncoated and fluorohydroxyapatite(FHA)-coated, were investigated in MG-63 osteoblasts. At 72 hours, cells proliferated on biomaterials more slowly than in the control group (p < 0.0001), the proliferation rate was higher on FHA-coated LR than uncoated HR (p = 0.037). Collagen-I production was positively affected by the LR surface (p = 0.001) as compared to controls, while it was significantly lower (p = 0.0001) in the HR surfaces. Compared to controls, LR and HR surfaces led to enhanced production of TGF-β1, further improved by FHA (FHA-coated LR: p = 0.007; FHA-coated HR p < 0.0001 respectively). ALP, OC, IL-6 and TNF-α levels were not significantly different from the controls. Results suggest that collagen-I production could be useful in predicting the in vivo osteointegration rate of biocompatible biomaterials observed in previous studies.
PuleoD.A., NanciA.Understanding and controlling the bone-implant interface. Biomaterials1999; 20: 2311–21.
2.
SandriniE., ChiesaR., RondelliG., SantinM., CigadaA.A novel biomimetic treatment for an improved osteointegration of titanim. Journal of Applied Biomaterials & Biomechanics2003; 1: 33–42.
3.
RoccaM., OrientiL., SteaS., MoroniA., FiniM., GiardinoR.Comparison among three different biocoatings for orthopaedic prostheses. An experimental animal study. Int J Artif Organs1998; 21: 553–8.
4.
GiavaresiG., FiniM., ChiesaR.Osseointegration of sandblasted or anodized hydrothermally-treated titanium implants: mechanical, histomorphometrical and bone hardness measurements. Int J Artif Organs2002; 25: 806–13.
5.
MoroniA., FaldiniC., PegreffiF., GianniniS.The effect of hydroxyapatite on bone ingrowth into porous-coated titanium implants. Hip International2002; 12: 153–7.
6.
WillmannG.The evolution of ceramics in total hip replacement. Hip International2000; 10: 193–203.
7.
LoupasisG., BirtwistleS.J., HydeI.D.Hydroxyapatite-coated threated acetabular prostheses. A five-year follow-up radiographic study. Hip International1997; 7: 11–6.
8.
MartinJ.Y., ScwartzZ., HummertT.W.Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells. J Biomed Mater Res1995; 29: 389–401.
9.
MartiniD., FiniM., FranchiM.Detachment of titanium and fluorohydroxyapatite particles in unloaded endosseous implants. Biomaterials2003; 24: 1309–16.
10.
VercaigneS., WolkeJ.G.C., NaertI., JansenJ.A.Histomor-phometrical and mechanical evaluation of titanium plasma-spray-coated implants placed in the cortical bone of goats. J Biomed Mater Res1998; 41: 41–8.
11.
MontanaroL., ArciolaC.R., CampocciaD., CervellatiM.In vitro effects on MG63 osteoblast-like cells following contact with two roughness-differing fluorohydroxyapatite-coated titanium alloys. Biomaterials2002; 23: 3651–9.
12.
DhertW.J.A., KleinCPAI, WolkeJ.G.C., van der VeldeE.A., de GrootK., RozingP.M.A mechanical investigation of fluoroapatite, magnesium whitlockite and hydroxyapatite plasma-sprayed coating in goats. J Biomed Mater Res1991; 25: 1183–200.
13.
FiniM., SavarinoL., Nicoli AldiniN.Biomechanical and histomorphometric investigations on two morphologically differing titanium surfaces with and without fluorohydroxyapatite coating. An experimental study in sheep tibia. Biomaterials2003; 24: 3183–92.
14.
SavarinoL., FiniM., CiapettiG.Biological effects of surface roughness and fluorohydroxyapatite coating on osteointegration in external fixation systems: An in vivo experimental study. J Biomed Mater Res (in press).
15.
RanzX., ReyC., AntolottiN.Properties of plasma sprayed bioactive fluorohydroxyapatite coatings. In: SedelL., ReyC., eds. Bioceramics.Paris: Elsevier Science Ltd, 1997; p. 10.
16.
SchmidtC., IgnatiusA.A., ClaesL.E.Proliferation and differentiation parameters of human osteoblasts on titanium and steel surfaces. J Biomed Mater Res2001; 54: 209–15.
17.
NishioK., NeoM., AkiyamaH.The effect of alkali-and heat trated titanium and apatite-formed titanium on osteoblastic differentiation of bone marrow cells. J Biomed Mater Res2000; 52: 652–61.
18.
MandracchiaV.J., NelsonS.C., BarpE.A.Current concepts of bone healing. Clin Pediatr Med Surg2001; 18: 55–77.
19.
BarnesG.L., KostenuikP.J., GerstenfeldL.C., EinhornT.A.Growth factor regulation of fracture repair. J Bone Miner Res1999; 14: 1805–15.
20.
KapanenA., KinnunenA., RyhanenJ., TuukkanenJ.TGF-β1 secretion of ROS-17/2.8 cultures on NiTi implant material. Biomaterials2002; 23: 3341–6.
21.
ShidaJ., TrindadeM.C.D., GoodmanS.B., SchurmanD.J., SmithR.L.Induction of interleukin-6 release in human osteoblast-like cells exposed to titanium particles in vitro. Calcif Tissue Int2000; 67: 151–5.
22.
TakeiH., PiolettiP.D., KwonS.Y., SungK.L.P.Combined effect of titanium particles and TNFα on the production of IL-6 by osteoblast-like-cells. J Biomed Mater Res2000; 382–7.
23.
WangW., SabokbarA., SimpsonAHRW, AthanasouN.A.The effect of interleukin-1β, tumor necrosis factor α and particle phagocytosis on monocyte-osteoclast differentiation. Hip International1999; 9: 178–85.
24.
CampocciaD., ArciolaC.R., CervellatiM., MaltarelloM.C., MontanaroL.In vitro behaviour of bone-marrow derived mesenchymal cells cultured on fluorohydroxyapatite-coated substrata with different roughness. Biomaterials2003; 24: 587–96.
25.
BoyanB.D., SylviaV.L., LiuY.Surface roughness mediates its effects on osteoblasts via protein kinase A and phospholipase A2. Biomaterials1999; 20: 2305–10.
26.
OrsiniG., AssenzaB., ScaranoA., PiattelliM., PiattelliA.Surface analysis of machined versus sandblasted and acid-etched titanium implants. Int J Oral Maxillofac Implants2000; 15: 779–84.
27.
GiardinoR., FiniM., GiavaresiG.Experimental surgery in the research of artificial organs. Int J Artif Organs1998; 21: 506.
28.
GiardinoR., FiniM., OrientiL.Laboratory evaluations for artificial organ evaluation. Int J Artif Organs1997; 20: 76–80.
29.
FiniM., GiardinoR.In vitro and in vivo tests for orthopaedic material biological evaluation: benefits and limits. Journal of Applied Biomaterials & Biomechanics2003 (in press).
30.
AlbrektssonT., JohanssonC.Osteoinduction, osteo-conduction and osseointegration. Eur Spine J2001; 10(suppl): S96–101.
31.
VermesC., ChandrasekaranR., JacobsJ.J., GalanteJ.O., RoebuckK.A., GlantT.T.The effects of particulate wear debris, cytokine, and growth factors on the function of MG-63 osteoblasts. J Bone Joint Surg (Am)2001; 83–A: 201-11.
