Long-Evans (Eker) rats carry a mutation that predisposes them to develop spontaneous renal cell tumors of two morphologic patterns: solid chromophilic masses or cystic lesions lined by eosinophilic cells. Previous studies have suggested that these tumors arise from the proximal tubules. In the present study, lectin-binding characteristics and cytokeratin expression of various stages of hereditary rat renal epithelial neoplasia were examined to localize the portion of the nephron from which tumors arise. Lectin-binding histochemistry has been used as a marker of cell surface glycoprotein expression, thought to be important in the differentiation of benign from malignant epithelial lesions and in the determination of their cell of origin. The presence or absence of keratin intermediate filaments in the rat nephron has been used to identify nephron segments. The polyclonal antibody to high- and low-molecular-weight cytokeratin stained the cells of the collecting ducts but not the proximal or distal tubules. Binding to the proximal tubules by the lectins Conavalia ensiformis (Con A), Dolichas biflorus, Ricinus communis (RCA-1), and Triticum vulgare and to the distal tubules by Con A, RCA-1, Arachis hypogaea (PNA) with and without neuraminidase, and the antibody for cytokeratins was demonstrated. The lectin binding and cytokeratin staining patterns of rat hereditary renal cell carcinoma, adenoma and the preneoplastic lesions of atypical tubules and hyperplasias suggest that cystic adenomas arise from the distal nephron, principally the collecting duct, whereas the solid atypical tubules, hyperplasias, and adenomas arise from the proximal nephron, principally the proximal tubule.
BachmannSKrizWKuhnCFrankeWW:
Differentiation of cell types in the mammalian kidney by immunofluorescence microscopy using antibodies to intermediate filament proteins and desmoplakins.
Histochemistry77:
365–394,
1983.
BannaschPZerbanH:
Renal cell adenoma and carcinoma, rat. In:
Urinary System,
ed. JonesTCMohrUHuntRD,
pp. 112–139.
Springer-Verlag,
Berlin, Germany,
1986.
4.
BonsibSMBromleyCLagerJL:
Renal oncocytoma: diagnostic utility of cytokeratin-containing globular filamentous bodies.
Mod Pathol4:
16–23,
1991.
5.
BychkovVDolanJRReddyVB:
Lectin binding to common epithelial tumors of the ovaries.
Gynecol Obstet Invest13:
166–171,
1991.
6.
CastagnaroMAlroyJUcciAAGlewRH:
Lectin histochemistry and ultrastructure of feline kidneys from six different storage diseases.
Virchows Arch B Cell Pathol54:
16–26,
1987.
7.
CohenCMcCuePADerosePB:
Histogenesis of renal cell carcinoma and renal oncocytoma: an immunohistochemical study.
Cancer62:
1946–1951,
1988.
8.
DamjanovI:
Lectin cytochemistry and histochemistry.
Lab Invest57:
5–16,
1987.
9.
DietrichDRSwenbergJA:
Preneoplastic lesions in rodent kidney induced spontaneously or by non-genotoxic agents: predictive nature and comparison to lesions induced by genotoxic carcinogens.
Mutat Res248:
239–260,
1991.
10.
DomizioPRisdonRA:
Cystic renal neoplasms of infancy and childhood: a light microscopical, lectin histochemical and immunohistochemical study.
Histopathology19:
199–209,
1991.
11.
DrozDZacherDCharbitLGogusevJChretienYIrisL:
Expression of the human nephron differentiation molecules in renal cell carcinomas.
Am J Pathol137:
895–905,
1990.
EkerRMossigeJ:
A dominant gene for renal adenomas in the rat.
Nature189:
858–859,
1961.
14.
EkerRMossigeJJohannessenJVAarsH:
Hereditary renal adenomas and adenocarcinomas in rats.
Diagn Histopathol4:
99–110,
1981.
15.
EverittJIGoldsworthyTLWolfDCWalkerCW:
Hereditary renal cell carcinoma in the Eker rat: a rodent familial cancer syndrome.
J Urol148:
1932–1936,
1992.
16.
FaraggianaTBernsteinJStraussLChurgJ:
Use of lectins in the study of histogenesis of renal cysts.
Lab Invest53:
575–579,
1985.
17.
FernandesBSagmanUAugerMDemetrioMDennisJW:
Beta 1-6 branched oligosaccharides as a marker of tumor progression in human breast and colon neoplasia.
Cancer Res51:
718–723,
1991.
18.
GoldfarbSPughTD:
Morphology and anatomic localization of renal microneoplasms and proximal tubule dysplasias induced by four different estrogens in the hamster.
Cancer Res50:
113–119,
1990.
19.
GoldsteinIJPoretzRD:
Isolation, physio-chemical characterization and carbohydrate-binding specificity of lectins. In:
The Lectins, Properties, Functions and Applications in Biology and Medicine,
ed. LernerIESharonNGoldsteinIJ,
pp. 33–247.
Academic Press,
New York, NY,
1986.
20.
HaniokaKImaiYWatanabeMItoH:
Lectin histochemical studies on renal tumors.
Kobe J Med Sci36:
1–21,
1990.
21.
HinoOKlein-SzantoJPFreedJJTestaJRBrownDQVilenskyMYeungRSTartoffKDKnudsonAG:
Spontaneous and radiation-induced renal tumors in the Eker rat model of dominantly inherited cancer.
Proc Natl Acad Sci USA90:
327–331,
1993.
22.
HolthöferH:
Lectin binding sites in kidney: a comparative study of 14 animal species.
J Histochem Cytochem31:
531–537,
1983.
23.
HolthöferHVirtanenIPetterssonETörnrothTAlfthanOLinderEMiettinenA:
Lectins as fluorescence microscopic markers for saccharides in the human kidney.
Lab Invest45:
391–399,
1981.
24.
HsuSMRaineL:
Versatility of biotin-labeled lectins and avidin-biotin-peroxidase complex for localization of carbohydrate in tissue sections.
J Histochem Cytochem30:
157–161,
1982.
25.
ItoYFukatsuAYoshidaFWatanabeYMiyakawaKSakamotoNBrentjensJRMatsuoS:
Histochemical and immunohistochemical studies of diseased human glomeruli.
Jpn J Nephrol33:
17–26,
1991.
26.
KragelPJWaltherMMPestanereJPFilling-KatzMR:
Simple renal cysts, atypical renal cysts, and renal cell carcinoma in von Hippel-Lindau disease: a lectin and immunohistochemical study in six patients.
Mod Pathol4:
210–214,
1991.
27.
MurakamiT:
Histochemical study on lectin binding in surface epithelial tumor of the ovary.
Nippon Sanka Fujinka Gakkai Zasshi43:
328–334,
1991.
28.
NogueiraEKlimekFWeberEBannaschP:
Collecting duct origin of rat renal clear cell tumors.
Virchows Arch B Cell Pathol57:
275–283,
1989.
29.
OberleyTDGonzalezALauchnerLJOberleyLWLiJJ:
Characterization of early kidney lesions in estrogen-induced tumors in the Syrian hamster.
Cancer Res51:
1922–1929,
1991.
30.
OosterwijkEVan MuijenGNPOosterwijk-WakkaJCWarnaarSO:
Expression of intermediate-sized filaments in developing and adult human kidney and in renal cell carcinoma.
J Histochem Cytochem38:
385–392,
1990.
31.
OrtmannMVierbuchenM:
Lectin histochemistry in the kidney and renal tumors.
Verh Dtsch Ges Pathol73:
339–349,
1989.
32.
OrtmannMVierbuchenMFischerR:
Sialylated glycoconjugates in chromophobe cell renal carcinoma compared with other renal cell tumors.
Virchows Arch B Cell Pathol61:
123–132,
1991.
33.
SasanoHSaitoYNaguraHKudoRRojasMSilverbergSG:
Lectin histochemistry in mucinous and serous ovarian neoplasms.
Int J Gynecol Pathol10:
252–259,
1991.
34.
SharonNLisH:
Lectins: cell agglutinating and sugar-specific proteins.
Science177:
949–959,
1972.
35.
SilvaFGNadasdyTLaszikZ:
Immunohistochemical and lectin dissection of the human nephron in health and disease.
Arch Pathol Lab Med117:
1233–1239,
1993.
36.
SuzukiKMurakitaHHaginoMSatouFKatouTKohliY:
Mucosubstance and lectin histochemistry of DMH induced colonic tumor in rats.
Nippon Shokakibyo Gakkai Zasshi88:
1404–1412,
1991.
37.
ThoenesWStorkelSRumpeltHJ:
Histopathology and classification of renal tumors (adenomas, oncocytomas and carcinomas).
Pathol Res Pract181:
125–143,
1985.
38.
TruongLDPhungVTYoshikawaYMattioliCA:
Glycoconjugates in normal human kidney. A histochemical study using 13 biotinylated lectins.
Histochemistry90:
51–60,
1988.
39.
TurusovVSChemerisGY:
Renal cell tumors induced in CBA male mice by 1,2-dimethylhydrazine.
Toxicol Pathol20:
570–575,
1992.
40.
UlrichWHorvatRKrischK:
Lectin histochemistry of kidney tumours and its pathomorphological relevance.
Histopathology9:
1037–1050,
1985.
41.
VierbuchenMKleinPJUhlenbruckGKleinHOSchaeferHEFischerR:
The significance of lectin receptors in the kidney and in hypernephroma (renal adenocarcinoma).
Recent Results Cancer Res75:
68–75,
1980.
42.
WalkerCGoldsworthyTLWolfDCEverittJ:
Predisposition to renal cell carcinoma due to alteration of a cancer susceptibility gene.
Science255:
1693–1695,
1992.
43.
ZhengAJiZM:
Studies on the location of eight lectins in breast carcinoma.
Chung-hua Ping Li Hsueh Tsa Chih19:
275–278,
1990.
