Sections of formalin-fixed, paraffin-embedded canine leiomyomas, leiomyosarcomas, or fibrosarcomas were examined by immunohistochemical methods for the presence of desmin. Twenty-two leiomyomas and leiomyosarcomas were stained using the avidin-biotin complex technique, and 14 samples demonstrated positive staining for desmin. The eight negative results obtained may reflect differences in fixation or the affinity of the primary antibody for the tissues examined. Desmin was specific for myogenic tissues. Five canine fibrosarcomas examined immunohistochemically were all negative for desmin staining. The results indicate that desmin is a useful marker for immunohistochemical identification of canine leiomyomas and leiomyosarcomas.
References
1.
AltmannsbergerMOsbornMSchauerAWeberK:
Antibodies to different intermediate filament proteins: cell type-specific markers on paraffin-embedded human tissues.Lab Invest45:
427–434,
1981
2.
AltmannsbergerMOsbornMDroeseMWeberKSchauerA:
Diagnostic value of intermediate filament antibodies in clinical cytology.Klin Wochenschr62:
114–123,
1984
3.
AltmannsbergerMOsbornMTreunerJHilescherAWeberKSchauerA:
Diagnosis of human childhood rhabdomyosarcoma by antibodies to desmin, the structural protein of muscle specific intermediate filaments.Virchows Arch (Cell Pathol)39:
203–215,
1982
4.
AltmannsbergerMWeberKDrosteROsbornM:
Desmin is a specific marker for rhabdomyosarcomas of human and rat origin.Am J Pathol118:
85–95,
1985
5.
BaumalRKahnHJBaileyDPhillipsMJ:
The value of immunohistochemistry in increasing diagnostic precision of undifferentiated tumours by the surgical pathologist.Histochem J16:
1061–1078,
1984
6.
BrozmanM:
Immunohistochemical analysis of formaldehyde and trypsin or pepsin treated material.Acta Histochem63:
S251–260,
1978
CooperDSchermerASunT:
Classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications and limitations.Lab Invest52:
243–256,
1985
9.
CurranRCGregoryJ:
The unmasking of antigens in paraffin sections of tissue by trypsin.Experientia33:
1400–1401,
1977
10.
DebusEWeberKOsbornM:
Monoclonal antibodies to desmin, the muscle-specific intermediate filament protein.EMBO J12:
2305–2312,
1983
11.
DenkHKreplerRArtliebUGabbianiGRungger-BrandleELeonciniPFrankeWW:
Proteins of intermediate filaments: an immunohistochemical and biochemical approach to the classification of soft tissue tumors.Am J Pathol110:
193–208,
1983
12.
DickTWolfCCarpenterP:
Modifications of an immunoperoxidase method for enhanced antigen reactivity.J Histotech7:
201–202,
1984
13.
Du BoulayCEH:
Immunohistochemistry of soft tissue tumours: a review.J Pathol146:
77–94,
1985
14.
ErlandsonRA:
Diagnostic immunohistochemistry of human tumors.Am J Surg Pathol8:
615–624,
1984
GabbianiGKapanciYBarazzonePFrankeW:
Immunochemical identification of intermediate-sized filaments in human neoplastic cells.Am J Pathol104:
206–216,
1981
17.
GiornoR:
A comparison of two immunoperoxidase staining methods based on the avidin-biotin interaction.Diag Immunol2:
161–166,
1984
18.
HsuSRaineLFangerH:
A comparative study of the PAP method and an ABC method for studying polypeptide hormones with radioimmunoassay antibodies.Am J Clin Pathol75:
734–738,
1981
19.
HsuSRaineLFangerH:
Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.J Histochem Cytochem29:
577–580,
1981
20.
HuangSMinassianHMoreJD:
Application of immunofluorescent staining on paraffin sections improved by trypsin digestion.Lab Invest35:
383–390,
1976
21.
HullandTJ:
Tumors of muscle.In:
Tumors in Domestic Animals,
ed. MoultonJE,
2nd ed.,
p. 76.
University of California Press, Ltd,
London, England,
1978
22.
LazaridesE:
Intermediate filaments as mechanical integrators of cellular space.Nature283:
249–256,
1980
23.
MeittinenMLehtoVPBadleyRAVirtanenI:
Expression of intermediate filaments in soft tissue sarcomas.Intl J Cancer30:
541–546,
1982
24.
MukaiKRosaiJHallawayB:
Localization of myoglobin in normal and neoplastic human skeletal muscle cells using an immunoperoxidase method.Am J Surg Pathol3:
373–376,
1979
25.
NadjiMMoralesA:
Immunoperoxidase. Part 1. The technique and its pitfalls.Lab Med14:
767–771,
1983
26.
OsbornMWeberK:
Intermediate filaments: cell-type-specific markers in differentiation and pathology.Cell31:
303–306,
1982
27.
OsbornMWeberK:
Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology.Lab Invest48:
372–394,
1983
28.
RamaekersFCSPutsJJGMoeskerOKantA:
Antibodies to intermediate filament proteins in immunohistochemical identification of human tumours: an overview.Histochem J15:
691–713,
1983
29.
RussoJ:
Immunocytochemistry in Tumor Diagnosis.
Martinus Nijhoff Publishing Co,
Boston,
1985
30.
SaidJ:
Immunohistochemical localization of keratin proteins in tumor diagnosis.Hum Pathol14:
1017–1019,
1983
31.
SchmidEOsbornMRungger-BrandleEGabbianiGWeberKFrankeWW:
Distribution of vimentin and desmin filaments in smooth muscle tissue of mammalian and avian aorta.Exp Cell Res137:
329–340,
1982
