The chemical composition of the intervertebral disc of 9-month-old chondrodystrophoid and nonchondrodystrophoid dogs was studied for collagen, noncollagenous protein and glycosaminoglycan. Content of these substances differed significantly between breeds. The differences were most marked in the nucleus pulposus; the noncollagenous protein content of the nonchondrodystrophoid breed was higher than in that of the chondrodystrophoid dogs. The total nitrogen value of the nonchondrodystrophoid nuclei pulposi was less than that of the corresponding chondrodystrophoid discs mainly because of the high collagen content of the latter discs. Histochemically, it was found that the nuclei pulposi of the nonchondrodystrophoid breed contains larger amounts of glycosaminoglycan than in the discs of the chondrodystrophoid breeds.
References
1.
AlmlofJ.:
On achondroplasia in the dog.Zentbl. Vet. Med.8:
43–56(1961)
2.
AntonopoulosC. A.GardellS.SzirmaiJ. A.de TyssonskE. R.:
Determination of glycosaminoglycans (mucopolysaccharides) from tissues on the microgram scale.Biochim. biophys. Acta83:
1–9(1964)
3.
BitterT.MuirH. M.:
A modified uronic acid carbazole reaction.Analyt. Biochem.4:
330–334(1962)
4.
BraundK. G.:
Some aspects of structure and function of the canine intervertebral disc;
Ph.D. Thesis Sydney
(1974)
5.
BraundK. G.GhoshP.TaylorT. K. F.LarsenL. H.:
Morphological studies of the canine intervertebral disc. The assignment of the beagle to the achondroplastic classification.Res. vet. Sci.19:
167–172(1975)
6.
BraundK. G.GhoshP.TaylorT. K. F.LarsenL. H.:
Identification and quantitation of connective tissue hexosamines using a combination of gas-liquid chromatographic and colorimetric procedures.Analyt. Biochem.64:
10–17(1975)
7.
BraundK. G.GhoshP.TaylorT. K. F.LarsenL. H.:
The qualitative assessment of glycosaminoglycans in the canine intervertebral disc using a critical electrolyte concentration (CEC) staining technique.
Res. vet. Sci.
(in press)
8.
BuddeckeE.SziegoleitM.:
Isolierung, chemische Zusammensetzung und altersabhängige Verteilung von Mucopolysacchariden menschlicher Zwischenwirbelscheiben.Hoppe Seyler's Z. physiol. Chem.337:
66–78(1964)
9.
DavidsonE. A.SmallW.:
Metabolism in vivo of connective mucopolysaccharides. I. Chondroitin sulphate C and keratosulphate of nucleus pulposus.Biochim. biophys. Acta69:
445–452(1963)
ElsonL. A.MorganS. T. J.:
A colorimetric method for the determination of glycosamine and chondrosamine.Biochemistry, N. Y.27:
1824–1828(1933)
12.
GardellS.RastgeldiS.:
On the mucopolysaccharides of nucleus pulposus.Acta chem. scand.8:
362–363(1954)
13.
GhoshP.TaylorT. K. F.YarrollJ. M.BraundK. G.LarsenL. H.:
Genetic factors in the maturation of the canine intervertebral disc.Res. vet. Sci.19:
304–311(1975)
14.
GottschalkA.:
The chemistry and biology of sialic acids and related substances
(Cambridge University Press,
Cambridge1959)
15.
GowerW. E.PedriniV.:
Age related variations in proteinpolysaccharides from human nucleus pulposus, annulus fibrosus and costal cartilage.J. Bone Jt Surg.51A:
1154–1162(1969)
16.
HallD. A.LloydP. F.HappeyF.HortonW. G.NaylorA.:
Mucopolysaccharides of human nuclei pulposi.Nature, Lond.179:
1078–1079(1952)
17.
HallenA.:
Hexosamine and ester sulphate content of the human nucleus pulposus at different ages.Acta chem. scand.12:
1869–1872(1958)
18.
HallenA.:
Extraction of mucopolysaccharides from connective tissue.Acta chem. scand.14:
1828–1832(1960)
19.
HallenA.:
The collagen and ground substance of human intervertebral disc at different ages.Acta chem. scand.16:
705–710(1962)
20.
HansenH. J.:
A pathologic-anatomical study on disc degeneration in dog.Acta orthop. scand., suppl.11(1952)
21.
HansenH. J.:
Comparative views on the pathology of disc degeneration in animals.Lab. Invest.8:
1242–1266(1959)
22.
HashimotoA.LudowiegJ. J.:
The protein polysaccharides from the nucleus pulposus of whale intervertebral discs. Effect of disulphide reducing agents.Biochemistry, N. Y.7:
2469–2475(1968)
23.
HenryN. G. C.:
The hydration of the nucleus pulposus and its relation to intervertebral disc derangement.J. Bone Jt Surg.40B:
132–144(1958)
24.
HirschC.PaulsonS.SylvenB.SnellmanO.:
Biophysical and physiological investigations on cartilage and other mesenchymal tissue; characteristics of human nuclei during aging.Acta orthop. scand.22:
175–183(1952)
25.
HoerleinB. F.:
Canine neurology – diagnosis and treatment;
2nd ed.,
p. 321
(Saunders,
Philadelphia1971)
26.
LowtherD. A.BaxterE.:
Isolation of a chondroitin sulphate protein complex from bovine intervertebral discs.Nature, Lond.211:
595–597(1964)
27.
LyonsH.:
Changes in the protein polysaccharide fractions of nucleus pulposus from human intervertebral disc with age and disc herniation.J. Lab. clin. Med.68:
930–939(1966)
28.
LyonsH.JonesE.QuinnF. E.SpruntP. H.:
Protein polysaccharide complexes of normal and herniated human intervertebral discs.Proc. Soc. exp. Biol. Med.115:
610–614(1964)
29.
McKenzieN. A.WallaceH. S.:
The Kjeldahl determination of nitrogen. A critical study of digestion conditions, temperature, catalyst and oxidising agent.Aust. J. Chem.7:
55–70(1954)
30.
MalmgrenH.SylvenB.:
Biophysical and physiological investigations on cartilage and other mesenchymal tissues.V. Identification of the polysaccharide of bovine nuclei pulposi. Biochim. biophys. Acta9:
706–707(1952)
31.
MitchellP. E. G.HenryN. G. C.BillewiczW. Z.:
The chemical background of intervertebral disc prolapse.J. Bone Jt Surg.43B:
141–151(1961)
32.
RussellS. W.GriffithsR. C.:
Recurrence of cervical disc syndrome in surgically and conservatively treated dogs.J. Am. vet. med. Ass.153:
1412–1417(1968)
33.
SchlossB.:
Colorimetric determination of glucosamine.Analyt. Chem.23:
1321–1325(1951)
34.
ScottJ. E.DorlingJ.:
Differential staining of acid glycosaminoglycans (mucopolysaccharides) by alcian blue in salt solution.Histochemistry5:
221–233(1965)
35.
StegemannH.StalderK.:
Determination of hydroxyproline.Clinica chim. Acta18:
267–273(1967)
