Granular cell tumor, usually a benign neoplasm, has been the object of many studies because of its uncertain histogenesis and based on many immunohistochemical and ultrastructural studies it has been suggested that it originates from the Schwann cell. Our recent observation that granular cell tumor is positive with PG-M1, a new anti-macrophage monoclonal antibody, led us to further investigate the immunophenotypic profile of the tumor.
Study design
We studied 11 granular cell tumors using a panel of 20 antibodies, 13 monoclonal and 7 polyclonal.
Results
The immunohistochemical study showed in all cases a constant diffuse positivity for S-100 protein, neuron-specific enolase, vimentin, KP1 and PG-M1, as well as occasional and focal positivity for alpha-1-antitrypsin, alpha-1-antichymotrypsin and lysozyme.
Conclusions
The immunophenotypic profile constantly observed could be the expression, on one hand, of the neuroectodermic nature of the neoplasm, proven by positivity for S-100 protein, neuron specific enolase and vimentin, and on the other could be the expression of the phagocytic activity of the tumor cell, proven by positivity for KP1 and PG-M1 antibodies and also by the presence of numerous phagolysosomes.
AbrikossoffA.P.: Über Myome ausgehend von der quergestreiften willkürlichen Muskulatur.Virchows Arch. A, 260: 215–233, 1926.
2.
ArminA., ConnellyE.M., RowdenG.: An immunoperoxidase investigation of S-100 protein in granular cell myoblastomas: evidence for Schwann cell derivation.Am. J. Clin. Pathol., 79: 37–44, 1983.
3.
AzzopardiJ.G.: Histogenesis of the granular cell “myoblastoma”. J.Pathol. Bacteriol., 71: 85–94, 1956.
4.
BigottiG., StiglianoV.: Immunohistochemistry of granular cell myoblastoma.J. Exp. Clin. Cancer Res., 5: 359–364, 1986.
BuleyI.D., GatterK.C., KellyP.M.A., HeryetA., MillardP.R.: Granular cell tumors revisited. An immunohistological and ultrastructural study.Histopathology, 12: 263–274, 1988.
7.
CordellJ., FaliniB., ErberW., GhoshA., AbdulazizZ., MacdonaldS., MacdonaldS., PulfordK., SteinH., MasonD.Y.: Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J.Histochem. Cytochem., 32: 219–229, 1984.
8.
EnghardtM.H., JordanS.E.: Granular cell tumor of a digital nerve.Cancer, 68: 1764–1769, 1991.
9.
FaliniB., FlenghiL., PileriS.A., GambacortaM., SteinH., CavaliereA., DurkopH., MartelliM.F., PoggiS.: PG-M1: a new monoclonal antibody directed against a fixative-resistent epitope of a macrophage-restricted antigen.Am. J. Pathol., 142: 1359–1372, 1993.
10.
FisherE.R., WechslerH.: Granular cell myoblastoma - a misnomer. Electron microscopic and histochemical evidence concerning its Schwann cell derivation and nature (granular cell schwannoma).Cancer, 15: 936–954, 1962.
11.
GangemiP., ArcidiaconoA., PuzzoL.: Tumore a cellule granulose (mioblastoma) della corda vocale.Studio immunoistochimico. Pathologica, 78: 641–646, 1986.
12.
MazurM.T., ShultzJ.J., MyersJ.L.: Granular cell tumor. Innunohistochemical analysis of 21 benign tumors and one malignant tumor.Arch. Pathol. Lab. Med., 114: 692–696, 1990.
13.
MiettinenM., LehtonenE., LehtolaH., EkblomP., LehtoV.-P., VirtanenI.: Histogenesis of granular cell tumour. An immunohistochemical and ultrastructural study.J. Pathol., 142: 221–229, 1984.
14.
MittalK.R., TrueL.D.: Origin of granules in granular cell tumor. Intracellular myelin formation with autodigestion. Arch, Pathol.Lab. Med., 112: 302–303, 1988.
15.
MukaiM.: Immunohistochemical localization of S-100 proteins and peripheral nerve myelin protein (P2 protein, PO protein) in granular cell tumors.Am. J. Pathol., 112: 139–146, 1983.
16.
NakazatoY., IshizekiJ., TakahashiK., YamaguchiH.: Immunohistochemical localization of S-100 protein in granular cell myoblastoma.Cancer, 49: 1624–1628, 1982.
17.
NathrathW.B.J., RembergerK.: Immunohistochemical study of granular cell tumours. Demonstration of neurone-specific enolase, S-100 protein, laminin and alpha-1-antichymotrypsin.Virchows Arch. A, 408: 421–434, 1986.
18.
OsborneP.T., GiltmanL.I., JenningsB.R., McClellanG.: Absence of myelin basic protein in granular cell tumors.Acta Neuropathol., 73: 330–332, 1987.
19.
PearseA.G.E.: The histogenesis of granular cell myoblastoma (granular cell perineural fibroblastoma?). J.Pathol. Bacteriol., 62: 351–362, 1950.
20.
PenneysN.S., AdachiK., Ziegels-WeissmanJ., NadjiM.: Granular cell tumors of the skin contain myelin basic protein.Arch. Pathol. Lab. Med., 107: 302–303, 1983.
RodeJ., DhillonA.P., PapadakiL.: Immunohistochemical staining of granular cell tumour for neurone-specific enolase: evidence in support of a neural origin.Diagn. Histopathol., 5: 205–211, 1982.
23.
RohollP.J.M., KleyneJ., PijpersH.W., Van UnnikJ.A.M.: Comparative immunohistochemical investigation of markers for malignant histiocytes.Human Pathol., 16: 763–771, 1985.
24.
SeoI.S., AzzarelliB., WarnerT.F., GoheenM.P., SenteneyG.E.: Multiple visceral and cutaneous granular cell tumors. Ultrastructural and immunocytochemical evidence of Schwann cell origin.Cancer, 53: 2104–2110, 1984.
25.
ShimamuraK., OsamuraR.Y., UeyamaY., HataJ.I., TamaokiN., MachidaN., FukudaH., UemuraK.: Malignant granular cell tumor of the right sciatic nerve. Report of an autopsy case with electron microscopic, immunohistochemical, and enzyme histochemical studies.Cancer, 53: 524–529, 1984.
26.
SmolleJ., KonradK., KerlH.: Granular cell tumors contain myelin-associated glycoprotein. An immunohistochemical study using Leu 7 monoclonal antibody.Wirchows Arch. A, 406: 1–5, 1985.
27.
SoiniY., MiettinenM.: Widespread immunoreactivity for alpha-1-antichymotrypsin in different types of tumors.Am. J. Clin. Pathol., 89: 131–136, 1988.
28.
SternbergerL.A., HardyP.H., CuculisJ.J., MeyerH.J.: The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes.J. Histochem. Cytochem., 18: 315–333, 1970.
29.
TaylorC.R., MasonD.Y.: Immunohistological detection of intracellular immunoglobulin in formalin-paraffin sections from multiple myeloma using immunoperoxidase technique.Clin. Exper. Immunol., 18: 417–429, 1974.
UsuiM., IshiiS., YamawakiS., SasakiT., MinamiA., HizawaK.: Malignant granular cell tumor of the radial nerve. An autopsy observation with electron microscopic and tissue culture studies.Cancer, 39: 1547–1555, 1977.
