A human acute T lymphoblastic leukemia line (PF-382) was serially transplanted into nude mice. No takes were observed in untreated nude mice, whereas solid tumors were observed in splenectomized and total body, sublethally irradiated mice. The minimal tumor-inducing dose and the latency time remained unchanged after the third and fifth serial transplants. Moreover, leukemic cells recovered from the 8th in vivo passages displayed the same differentiation antigens and chromosomal markers as the in vitro PF-382 cell line used for the first transplant. This stable and well-characterized experimental system could be a new model for T-lymphocyte differentiation and immune-reactivity against human leukemias.
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References
1.
BalsariA., FossatiG., TaramelliD., NavaM., RavagnaniF., ParmianiG.: Inhibition of human melanoma growth in nude mice by autologous, alloactivated peripheral blood lymphocytes.Tumori, 70: 35–40, 1984.
2.
CampanileF., CrinoL., BonassardE., HouchensE., GoldingA.: Radioresistent inhibition of lymphoma growth in congenitally athymic (nude) mice.Cancer Res., 37: 394–389, 1977.
3.
ClarksonB., StrifeA., De HavrenE.: Continuous culture of seven new cell lines (SK-L 1 to 7) from patients with acute leukemia.Cancer, 20: 926–947, 1967.
4.
CudkowiczG.: Rejection of bone marrow allografts by irradiated athymic nude mice.Proc. Am. Assoc. Cancer Res., 16: 170–176, 1975.
5.
DiehlV., KrauseP., HellrigelK.P., BuscheM., ScedelI., LaskewitzE.: Lymphoid cell lines: in vitro cell markers in correlation to tumorigenicity in nude mice. In: Hematology and Blood Transfusion. Vol. 20, pp. 289–296, ThirhelderA., RodthF., ThielF. (eds.), Springer Verlag, Berlin, Heidelberg, New York, 1977.
6.
EpsteinA.L., HermanM.M., KimH., KaplanK.S.: Biology of the human malignant lymphomas. III. Intracranial heterotransplantation in nude athymic mouse.Cancer, 37: 2158–2176, 1976.
7.
FoghJ., GiovanellaB.C.: The Nude Mouse in Experimental and Clinical Research. Vol. 2. Academic Press, New York, 1982.
8.
ForniG., GiovarelliM.: In vitro reeducated T helper cells from sarcoma-bearing mice inhibit sarcoma growth in vivo.J. Immunol., 132: 527–533, 1984.
9.
GiovanellaB.C., NilssonK., ZechL.: Growth of diploid Epstein-Barr virus-carrying human lympho-blastoid cell lines heterotransplanted into nude mice under immunologically privileged conditions.Int. J. Cancer, 24: 103–110, 1979.
10.
HelsonL.: Pediatric tumor research with nude mice. In: The Nude Mouse in Experimental and Clinical Research.FoghJ., GiovanellaB.C. (eds.), Vol. 2, pp. 491–508, Academic Press, New York, 1982.
11.
IrvinA.D., BrownC.G., KanahaiG.K., StaagD.: Comparative growth of bovine lymphosarcoma cells and lymphoid cells infected with Theileria parva in athymic (nude) mice.Nature, 255: 713–714, 1975.
12.
LozzioB.L., MachadoE.A.: Transplantation and dissemination of human haematopoietic malignant cells in nude and lasat mice. In: Proc. 2nd Int. Workshop on Nude Mice.Nomura, pp. 587–594, University of Tokyo Press, Tokyo, 1982.
13.
NaraN., MiyamotoT.: Direct and serial transplantation of human acute myeloid leukemia into nude mice.Br. J. Cancer, 45: 778–782, 1982.
14.
OhsugiY., GerswhinM.E., OwensR.B., Nelson-ReesW.A.: Tumorigenicity of human malignant lymphoblasts: Comparative study with unmani-pulated nude mice, antilymphocyte serum-treated nude mice, and X-irradiated nude mice.J. Natl. Cancer Inst., 65: 715–718, 1980.
15.
PegoraroL., FierroM.T., LussoP., GiovinazzoB., LaninoE., GiovarelliM., MateraL., FoàR.: A novel leukemic T-cell line (PF-382) with phenotypic and functional features of suppressor lymphocytes.J. Natl. Cancer Inst., 75: 285–290, 1985.
16.
PegoraroL., PalumboA., EriksonJ., FaldaM., GiovinazzoB., EmanuelB.S., RoveraG., NowellP.C., CroceC.: A 14; 18 and an 8; 14 chromosome translocation in a cell line derived from an acute B-cell leukemia.Proc. Natl. Acad. Sci., USA, 81: 7166–7171, 1984.
17.
SchaadtM., KirchnerH., FonatschC.: Intracranial heterotransplantation of human haemopoietic cells in nude mice.Int. J. Cancer, 23: 751–761, 1979.
18.
SmithJ.: Biological behaviour of tumor lines. In: Proc. « Human Tumor Xenografts in Anticancer Drug Development ».Milan, 26-27 May 1986.
19.
SordatB., TamaokiN., PolvsenC.O.: List of human tumors transplanted to nude mice. In: Proc. 2nd Int. Workshop on Nude Mice, Nomura, pp. 587–595. University of Tokyo Press, Tokyo, 1977.
20.
WatanabeS., ShimosatoY., KurokiM., SatoY., NakajimaT.: Transplantability of human lymphoid cell line, lymphoma and leukemia in splenectomized and/or irradiated nude mice.Cancer Res., 40: 2588–2592, 1980.
