Epichlorohydrin (ECHH) highly inhibited the tritiated thymidine uptake by human lymphocytes cultured in vitro, although the corresponding cell viability was unaffected. Furthermore, it elicited unscheduled DNA synthesis, acting as a DNA-damaging agent after its metabolic activation. ECHH also showed a clear toxic and mutagenic activity toward a human epithelial-like cell line, causing a decrease in cell viability and an increase in mutants resistant to 0.05 Lf/ml of diphtheria toxin.
Get full access to this article
View all access options for this article.
References
1.
AmesB.N., McCannJ., YamasakiE.: Methods for detecting carcinogens and mutaagens with the Salmonella/mammalian-microsome mutagenicity test.Mutat. Res., 31: 347–364, 1975.
2.
BartschH., MalaveilleC., CamusA.M., Martel-PlancheG., BrunG., HautefeuilleA., SabadieN., BarbinA.: Validation and comparative studies on 180 chemicals with S. typhimurium strains and V 79 Chinese hamster cells in the presence of various metabolizing systems.Mutat. Res., 76: 1–50, 1980.
3.
FishbeinL.: Potential halogenated industrial carcinogenic and mutagenic chemicals.III. Alkane halides, alkanols and ethers. Sci. Total Environ., 11: 223–257, 1979.
4.
GlattH.R., SchwindH., ZajdelaF., CroisyA., JacquignonP.C., OeschF.: Mutageninicty of 43 structurally related heterocyclic compounds and its relationship to their carcinogenicity.Mutat. Res., 66: 307–328, 1979.
5.
GuptaR.S., SiminovitchL.: Isolation and characterization of mutants of human diploid fibroblasts resistant to diphtheria toxin.Proc. Natl. Acad. Sci. USA, 75: 3337–3340, 1978.
6.
I.A.R.C.: Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. Vol. 20, I.A.R.C., Lyon, France, 1979.
7.
KučerováM., PolívkováZ.: Banding technique used for the detection of chromosomal aberration induced by radiation and alkylating agents TEPA and Epichlorohydrin.Mutat. Res., 34: 279–290, 1976.
8.
KučerováM., PolívkováZ., ŠrámR., MatoušekV.: Mutagenic effect of epichlorohydrin.I. Testing on human lymphocytes in vitro in comparison with TEPA. Mutat. Res., 34: 271–278, 1976.
9.
KučerováM., ZhurkovV.S., PolívkováZ., IvanovaJ.E.: Mutagenic effect of epichlorohydrin.II. Analysis of chromosomal aberrations in lymphocytes of persons occupationally exposed to epichlorohydrin. Mutat. Res., 48: 355–360, 1977.
10.
LiebermanM.W., BaneyR.N., LeeR.E., SellS., FarberE.: Studies on DNA repair in human lymphocytes treated with proximate carcinogens and alkylating agents.Cancer Res., 31: 1297–1306, 1971.
11.
PeroccoP., ProdiG.: DNA damage by haloalkanes in human lymphocytes cultured in vitro.Cancer Letters., 13: 213–218, 1981.
12.
RocchiP., FerreriA.M., BorgiaR., ProdiG.: Polycyclic hydrocarbons induction of diphtheria toxin-resistant mutants in human cells.Carcinogenesis, 1: 765–767, 1980.
13.
RosenkranzH.S.: Mutagenicity of halogenated alkanes and their derivates.Environ. Health Perspect., 21: 79–84, 1977.
14.
SchumannA.M., WatanabeP.G.: Species differences between rats and mice on the metabolism and hepatic macromolecular binding of tetrachloroethylene.Toxicol. Appl. Pharmacol., 48: 789, 1979.
15.
ŠrámR.J., ZudováZ., KuleshovN.P.: Cytogenetic analysis of peripheral lymphocytes in workers occupationally exposed to epichlorohydrin.Mutat. Res., 70: 115–120, 1980.
16.
VoogdC.E., Van der StelJ.J., JacobsJ.J.J.A.A.: The mutagenic action of aliphatic epoxides.Mutat. Res., 89: 269–282, 1981.
17.
WhiteA.D.: In vitro induction of SCE in human lymphocytes by epichlorohydrin with and without metabolic activation.Mutat. Res., 78: 171–176, 1980.
