The specific binding of labelled human prolactin was determined in 83 human breast carcinomas. Twenty-seven tumors (32.5 %) contained specific binding for prolactin of at least 1 % and were considered prolactin receptor positive. The binding was found linearly related to membrane protein concentration and specific only for lactogenic hormones. By Scatchard analysis the dissociation constant appeared similar to that observed in other target tissues, with a low binding capacity.
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References
1.
BoltonA.E., HunterW.M.: The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agents.Biochem. J.,133: 529–539, 1973.
2.
CostlowM.E., McGuireW.: Prolactin receptors and hormone dependence in mammary carcinoma. In: Endocrine control in neoplasia. pp. 121–150. SharmaR.K., and CrissW.E. (eds.). Raven Press, New York, 1978.
3.
Di CarloR., MuccioliG., Di CarloF.: Estrogen and prolactin receptor concentrations in human breast tumours. Abstr. of International Symposium on « Pharmacological Modulation of Steroid Action », Turin, 23-25 July, 1978.
4.
FriesenH.G.: The role of prolactin in breast cancer.In: Recent Results in Cancer Research, vol. 57, pp. 143–149. St. ArneaultG., BondP., and IsräelL. (eds.). Springer, Berlin, 1976.
5.
HaydenT.J., BonneyR.C., ForsythI.A.: Ontogeny and control of prolactin receptors in the mammary gland and liver of virgin, pregnant and lactating rats.J. Endocrinol.,80: 259–269, 1979.
6.
HoldawayI.M., FriesenH.G.: Hormone binding by human mammary carcinoma.Cancer Res.,37: 1946–1952, 1977.
7.
LowryO.H., RosenbroughN.J., FarrA.L., RandallK.J.: Protein measurement with the folin phenol reagent.J. Biol. Chem.,193: 265–275, 1951.
PartridgeR.K., HahnelR.: Prolactin receptors in human breast carcinoma.Cancer,43: 643–646, 1979.
10.
PearsonO.H., ManniA.: Hormonal control of breast cancer growth in women and rats.In: Current Topics in Experimental Endocrinology, vol. 3, pp. 75–92. MartiniL., and JamesV.H.T. (eds.). Academic Press, New York and London, 1978.
11.
PearsonO., ManniA., ChambersM., BrodkeyJ., MarshallJ.S.: Role of pituitary hormones in the growth of human breast cancer.Cancer Res.,38: 4323–4326, 1978.
12.
ScatchardG.: The attractions of proteins for small molecules and ions. Ann. N.Y.Acad. Sci.,51: 660–672, 1949.
13.
ShiuR.P.C., FriesenH.G.: Properties of a prolactin receptor from the rabbit mammary gland.Biochem J.,140: 301–311, 1974.
14.
ShiuR.P.C., KellyP.A., FriesenH.G.: Radioreceptor assay for prolactin and other lactogenic hormones.Science,180: 968–971, 1973.
15.
StagnerJ.I., JochimsenP.R., ShermanB.M.: Lactogenic hormone binding to human breast cancer: Correlation with estrogen receptor.Clin. Res.,25: 302A, 1977.
16.
WatersM.J., FriesenH.G., BohnetH.G.: Regulation of prolactin receptors by steroid hormones and use of radioligand assays in endocrine research.In: Receptors and Hormone Action, vol. 3, pp. 457–477. BirnbaumerL., and O'MalleyB.W. (eds.). Academic Press, New York, San Francisco and London, 1978.
