Two or three weekly repeated intravenous injections of 7,12-dimethylbenz(a)-anthracene (DMBA) in 15% fat emulsion were given to adult randomly bred Syrian golden hamsters. The treatments resulted in the induction of ovarian tumors in the females with incidence of 77% and 85% in the twice and three times injected animals respectively. Out of the 45 ovarian tumors found in the treated groups, 23 were the granulosa cell type, 6 were the theca cell type, 15 were the granulosa-theca cell type (mixed) and one was a hilar cell tumor. In 35 animals the tumors occurred bilaterally and in 10 unilaterally.
Additionally, a significant percentage of animals of both sexes developed dermal melanocytomas, tumors of forestomach, breast, intestines, skin, subcutis, lymphoreticular tissue, etc.
The induction of ovarian tumors with high incidence by a chemical carcinogen in the hamster provides a model by which the factors involved in the pathogenesis of this neoplasm could be studied.
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