Abstract
The effect of a pyrimidine analog, the 2-thio-5-methylcytosine, on the synthesis of RNA and DNA nucleic fractions of Walker carcinosarcoma has been investigated by a modified autoradiographic technique and by radiochemical determinations. Results obtained by the two methods are illustrated and compared, and their meaning is discussed. It is pointed out that the occurrence of the induced effect is likely dependent on the hematic and tissue levels of the substance, according to the different administration ways.
The depressing action of thiopyrimidine on radiophosphorus uptake in the neoplastic tissue of the treated animals is confirmed. It is also observed that P32 inhibition, though more marked for the DNA fraction, is also present in the RNA fraction.
An interpretation is given of the correlation between autoradiographical and radiochemical results and histological ones (mitotic coefficient).
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