Changes in Granulocyte-Macrophage Colony Formation in Relation to Chemotherapy and Clinical Progress in Acute Myeloblastic Leukaemia Patients

Abstract

To investigate the correlation between granulocyte-macrophage colony formation and the prognosis of acute myelocytic leukaemia, an in vitro colony formation assay using a practical method was performed at diagnosis in 50 patients newly diagnosed with acute myelocytic leukaemia. Granulocyte-macrophage colony counts were significantly lower in acute myelocytic leukaemia patients than in the control group (n = 5). The diminished colony formation was restored to within the normal range in 15 patients after complete remission was achieved. In eight patients, serial evaluation of granulocyte-macrophage colony formation was performed, and suppression of colony formation was observed at relapse. The comparison of granulocyte-macrophage colony counts at diagnosis between a group of patients with complete remission (n = 26) and a group of treatment failure cases (n = 24) revealed a significant difference suggesting that high counts of granulocyte-macrophage colony formation are predictive of a favourable prognosis for acute myelocytic leukaemia.

Keywords

ACUTE MYELOCYTIC LEUKAEMIA GRANULOCYTE-MACROPHAGE COLONY FORMATION PROGNOSIS COMPLETE REMISSION RATE

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References

Moor

Spitzer

Williams

Agar culture studies in 127 cases of untreated acute leukaemia: the prognostic value of reclassification of leukaemia according to in vitro growth characteristics. Blood 1974; 44: 1–18.

Kubota

Mizoguchi

Miura

Serial studies on in vitro colony formation in patients with acute leukaemia in relation to the maintenance of remission. Am J Hematol 1978; 5: 285–290.

Queensberry

Hemopoietic stem cells, progenitor cells, and cytokines. In: Williams Hematology ( Beutler

Lichtman

Coller

Kipps

eds). New York: McGraw-Hill, Inc., 1995; pp211–228.

Bennett

Catovsky

Daniel

Proposal for the classification of the acute leukaemias. Br J Haematol 1976; 33: 451–458.

Bennett

Catovsky

Daniel

Proposal for the recognition of minimally differentiated acute myeloid leukaemia (AML-MO). Br J Haematol 1992; 81: 458–459.

Kawada

Tamura

Kubota

Possible involvement of protein kinase C in the aberrant regulation of erythropoiesis in polycythemia vera. Leukaemia Res 1997; 21: 101–105.

Dombret

Granulocytic colony-stimulating factors in the management of patients with acute myeloid leukemia. Haematol Cell Ther 1996; 38: 231–240.

Ohno

Kobayashi

Tanimoto

Randomized study of individualized induction therapy with or without vincristine, and of maintenance-intensification therapy between 4 or 12 courses in adult acute myeloid leukemia. Cancer 1993; 71: 3888–3894.

Kobayashi

Miyawaki

Tanimoto

Randomized trials between behenoyl cytarabine and cytarabine in combination induction and consolidation therapy, and with or without ubenimex after maintenance/intensification therapy in adult myeloid leukemia. J Clin Oncol 1996; 14: 204–213.