A Method Based on the Roller Chamber Technique for Determination of CO 2 Production in Cultured Cells: Effects of Acrylamide in Neuroblastoma N1E115 Cultures
Restricted accessResearch articleFirst published online April, 1991
A Method Based on the Roller Chamber Technique for Determination of CO 2 Production in Cultured Cells: Effects of Acrylamide in Neuroblastoma N1E115 Cultures
The effects of acrylamide on CO2 production from [14C]-labelled glucose, pyruvate and glutamine have been studied in a mouse neuroblastoma cell line C1300, clone N1E115. A rotation metabolism chamber, permitting closed incubation of monolayer, anchorage-dependent cell cultures under good physiological conditions, was developed for making the determinations. The cells were exposed to acrylamide (0.35mM) for 14 days. The total amunt of CO2 produced from glucose and pyruvate was increased by exposure to acrylamide, whereas a slight inhibition was found in the production from glutamine. The production of lactate remained unchanged. Comparison of these results with other data obtained in our laboratory leads us to conclude that the method described is relevant for the determination of energy metabolic processes through the quantification of CO2 production. Furthermore, we assume that acrylamide causes an increased demand for energy in the cells and that this demand is met by the cells through the increased oxidative phosphorylation of glucose.
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