Twenty-six chemicals supplied coded by FRAME, were tested on HEp-2 cells. Three endpoints were used to measure the cytotoxicity of these compounds: total protein content, LDH release and total acid phosphatase content.
The sensitivity of these parameters is discussed. The results are compared with those obtained by other groups participating in the validation scheme organised by FRAME.
KnoxP., UphillP.F., FryJ.R., BenfordD.J., and BallsM. (1986). The FRAME multicentre project on in vitro cytotoxicology.Food and Chemical Toxicology24, 457–463.
2.
BallsM., RiddellR.J., HornerS.A., and ClothierR.H. (1987). The FRAME approach to the development, validation and evaluation of in vitro alternative methods. In Alternative Methods in Toxicology, Vol. 4, Approaches to Validation (ed. GoldbergA.M.), pp. 45–58. New York: Mary Ann Leibert Inc.
3.
StammatiA., ZuccoF., CardelliM., De AngelisI., MatteiR., PinoA., RoccaE., and ZampaglioniF. (1985). Cytotoxic effects of wheat gliadin-derived peptides.Toxicology37, 225–232.
4.
De AngelisI., GiubileiL., StammatiA., ZampaglioniF., ZuccoF., BartoliniG., and SalvatoreG. (1986). In vitro toxicity of some cosmetic ingredients.Food and Chemical Toxicology24, 477–479.
5.
StammatiA., TrucchiD., ZampaglioniF., MacriA., and ZuccoF. (1988–1989). Cytotoxic effects of furazolidone on two cell lines.In Vitro Toxicology2, 273–286.
6.
StammatiA., and PirovanoR. (1988). In vitro cytotoxicity test on HEp-2 cells. In Collaborative Study on Relationship Between In Vivo Primary Irritation and In Vitro Experimental Models. EEC project 86E3-063/86-460, CEC/V/E/3/Lux/-157/88.
7.
RiddellR.J., ClothierR.H., and BallsM. (1986). An evaluation of three in vitro cytotoxicity assays.Food and Chemical Toxicology24, 469–471.
8.
GarleM., HammondA.H., and FryJ.R. (1987). The cytotoxicity of 27 chemicals to V79 Chinese hamster cells.ATLA15, 30–32.
9.
ClothierR.H., HulmeL., AhmedA.B., ReevesH.L., SmithM., and BallsM. (1988). In vitro cytotoxicity of 150 chemicals to 3T3-L1 cells assessed by the FRAME kenacid blue method.ATLA16, 84–95.
10.
MatteiR., StammatiA., and ZuccoF. (1985). Acid phosphatase as a marker of cellular damage: characterisation of two cell lines for toxicity studies.Annali Istituto Superiore Sanitd21, 377–382.
11.
EkwallB., and JohanssonA. (1980). Preliminary studies on the validity of in vitro measurement of drug toxicity using HeLa cells. I. Comparative in vitro toxicity of 27 drugs.Toxicology Letters5, 299–307.
12.
RiddellR.J., PanacerD.S., WildeS.M., ClothierR.H., and BallsM. (1986). The importance of exposure period and cell type in in vitro cytotoxicity tests.ATLA14, 86–92.
13.
BallsM., and HornerS.A. (1985). The FRAME interlaboratory programme on in vitro cytotoxicology.Food and Chemical Toxicology23, 209–213.
