A roller incubation procedure was used to co-cultivate primary cultures of chick hepatocytes with a human fibroblast-like cell line (HFB). When HFB cells alone were incubated, in the presence of allyl alcohol, an increased rate of protein synthesis was observed. In the hepatocyte-fibroblast co-cultures, allyl alcohol caused a dose-dependent decrease in the rate of protein synthesis in the cells of both types. This effect was much more pronounced in the hepatocytes. It was concluded that in the co-cultures allyl alcohol was oxidised to acrolein, which exerted its toxic action primarily on the hepatic cells.
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2.
EricssonA.C., and WalumE. (1984). Cytotoxicity of cyclophosphamide and acrylamide in glioma and neuroblastoma cell lines co-cultured with liver cells.Toxicology Letters20, 251–256.
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WalumE., VarnboI., and PetersonA. (1986). A multiple cell culture toxicity test system based on neuroblastoma C1300 cells and differentiated primary cultures of brain, muscle, heart and liver cells.Food and Chemical Toxicology24, 567–568.
8.
NybergE., and WalumE. (1984). On the application of cultured neuronal cell-lines in neurotoxicological studies: implications of acylamide-induced neurite disintegration.ATLA11, 194–203.
9.
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