Abstract
Objective: Learning the levels of oxidative DNA damage in children with obstructive adenotonsillar hypertrophy. We determined the 8-hydroxy 2-deoxyguanosine levels in leukocyte DNA and in urine samples as a specific marker of oxidative damage to DNA, and the plasma and urine malondialdehyde levels as an index of lipid peroxidation in children.
Method: Prospective, randomized controlled study conducted between 2010 and 2011. The study consisted of 30 patients and 25 healthy controls. Patients had snoring and pauses of breathing during sleep. We obtained blood and urine samples from each child to measure 8-hydroxy 2-deoxyguanosine and malondialdehyde levels in their initial visit.
Results: A significant difference was observed in leukocyte and urine 8-hydroxy 2-deoxyguanosine levels in patients with obstructive adenotonsillar hypertrophy compared to controls (P < .001 for both). Similarly, a significant difference was observed in plasma and urine malondialdehyde levels in patients with obstructive adenotonsillar hypertrophy compared to controls (P < .001 for both). In addition, significant correlations were observed between the levels of 8-hydroxy 2-deoxyguanosine in leukocyte DNA and plasma malondialdehyde (r = 0.648, P < .001), and between the levels of urine 8-hydroxy 2-deoxyguanosine excretion and urine malondialdehyde (r = 0.588, P < .001).
Conclusion: 8-hydroxy 2-deoxyguanosine in leukocyte DNA and urine levels in children can be useful as an indicator for obstructive adenotonsillar hypertrophy screening.
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