Identification of Differentially Expressed Genes in Human Papillomavirus Type-16 Infected Oral Cancer Cells

Abstract

OBJECTIVE:

The goal of this study was to determine the genes required for head and neck cancer development.

STUDY DESIGN AND SETTING:

Differential mRNA display analysis was performed using human papillomavirus Type-16 infected immortalized human oral keratinocytes (HOK-16B) and its benzo(a)pyrene-exposed tumorigenic derivative (HOK-16B-BaP-T).

RESULTS:

Twenty-one differentially expressed cDNA clones were identified between the 2 cell lines. Clone 4 with no known homology showed lower expression in tumorigenic cells compared with either normal or immortalized oral keratinocytes. Clone 6 expression was elevated in several head and neck cancer cells, in addition to Burkitt's lymphoma Rajl harboring latent Epstein-Barr virus.

CONCLUSION:

These findings suggested that clone 6 may be involved in the oncogenic transformation whereas clone 4 may potentially function as a tumor suppressor gene.

SIGNIFICANCE:

Differential mRNA analysis using the in vitro oral carcinogenesis model may help to identify important genetic markers for the early detection and progression of head and neck cancer.

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