The National Institute of Environmental Health Sciences (NIEHS) supports a number of training programs for predoctoral and postdoctoral (D.V.M., M.D., Ph.D.) fellows in toxicology, epidemiology and biostatistics, and environmental pathology. At the Experimental Biology meeting in April 1997, the American Society of Investigative Pathology (ASIP) sponsored a workshop including directors, trainees, and other interested scientists from several environmental pathology programs in medical and veterinary colleges. This workshop and a related session on "Novel Cell Imaging Techniques for Detection of Cell Injury" revealed advances in molecular and cell imaging approaches as reviewed below that have a wide applicability to toxicologic pathology.
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Davis WP, Janssen Ymw, Mossman BT, and Taatjes DJ (1997). Simultaneous triple fluorescence detection of mRNA localization, nuclear DNA, and apoptosis in cultured cells using confocal laser scanning microscopy. Histochem. Cell Biol.108: 307-311.
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Florin EL, Moy VT, and Gaub HE ( 1994). Adhesion forces between individual ligand-receptor pairs . Science264: 415-417.
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Fung H., Kow YW, Van Houten B, Taatjes DJ, Hatahet Z., Janssen Ymw, Vacek P., Faux SP, and Mossman BT (1998). Asbestos increases mammalian major AP-endonuclease (APE) gene expression, protein levels and enzyme activity in rat pleural mesothelial cells. Cancer Res.58: 189-194.
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Goldberg JL, Zanella CL, Janssen Ymw, Timblin CR, Jimenez LA, Vacek P., Taatjes DJ, and Mossman BT (1997). Novel cell imaging approaches show induction of apoptosis and proliferation in mesothelial cells by asbestos. Am. J. Respir. Cell Mol. Biol.17: 265-271.
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Kamentsky LA and Kamentsky LD (1991). Microscope-based multiparameter laser scanning cytometer yielding data comparable to flow cytometry data. Cytometry12: 381-387.
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Pache JC, Janssen Ymw, Walsh ES, Quinlan TR, Zanella CL, Low RB, Taatjes DJ, and Mossman BT (1998). Increased epidermal growth factor-receptor (EGF-R) protein in a human mesothelial cell line in response to long asbestos fibers . Am. J. Pathol.152: 333-340.
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Radmacher M. , Fritz M., Hansma HG, and Hansma PK (1994). Direct observation of enzyme activity with the atomic force microscope. Science265: 1577-1579.
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Sewall CH, Clark GC, and Lucier GW (1995). TCDD reduces rat hepatic epidermal growth factor receptor: Comparison of binding, immunodetection, and autophosphorylation . Toxicol. Appl. Pharmacol.132: 263-272.
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Soultanakis RP, Cooper WC, Heintz NH, Mossman BT, and Janssen Ymw (1998). Cell cycle dependent expression of c-jun and c-jun-N-terminal kinase in rat lung epithelial cells and the effects of exposure to reactive oxygen or nitrogen species (ROS or RNS). Am. Rev. Respir. Crit. Care Med.157: A453 (abstract).
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Timblin CR, Janssen Ywm, and Mossman BT (1995). Transcriptional activation of the proto-oncogene c-jun, by asbestos and H2O2 is directly related to increased proliferation and transformation of tracheal epithelial cells. Cancer Res.55: 2723-2726.
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Zanella CL, Posada J., Tritton TR, and Mossman BT (1996). Asbestos causes stimulation of the ERK-1 mitogen-activated protein kinase cascade after phosphorylation of the epidermal growth factor receptor. Cancer Res.56: 5334-5338.
