One approach to assessment of the immunotoxic effects of drugs and chemicals upon the immune system is briefly described. Appropriate in vivo and in vitro methods and techniques are delineated. It is likely that programs implemented at individual institutions will be modified to specific circumstances.
References
1.
1. Casale TB, and Bigbee JW (1982). A rapid method for isolation of human mononuclear cells free of significant platelet contamination. J. Immunol. Methods55: 347–353.
2.
2. Cerruti-Sola T, Kristensen F, Vandevelde M, and de Weck AL (1984). Interleukin 1- and 2-like activities in the dog. Vet. Immunol. Immunopathol.6: 261–271.
3.
3. Dean JH, Bigbee JW, and Bigbee JW (1982). Methods and approaches for assessing immunotoxicity: An overview. Environ. Health Pers.43: 27–29.
4.
4. Ellens DJ, and Bigbee JW (1980). A simple method for the purification of 5-amino-salicylic acid. Application of the product as a substrate in ELISA. J. Immunol. Methods37: 325–332.
5.
5. Felsburg PJ, Bigbee JW, Bigbee JW, and Bigbee JW (1984). Polyclonal activation of canine B lymphocytes evaluated by a protein A reverse hemolytic plaque assay. Vet. Immunol. Immunopathol.6: 353–364.
6.
6. Habeek AFSA, and Bigbee JW (1984). Preparation of human immunoglobin by caprylic acid precipitation. Prep. Biochem.14: 1–17.
7.
7. Koller LD, and Bigbee JW (1983). Induction of humoral immunity to protein antigen without adjuvant in rats exposed to immunosuppressive chemicals. J. Toxicol. Environ. Health12: 173–181.
8.
8. Krakowka S, Bigbee JW, and Bigbee JW (1975). Effects of canine distemper virus on lymphoid function in vitro and in vivo. J. Infect. Immun.11: 1069–1078.
9.
9. Krakowka S, Bigbee JW, Bigbee JW, and Bigbee JW (1978). Evaluation of B-lymphocyte levels and functions in gnotobiotic dogs. Am. J. Vet. Res.39: 1181–1183.
10.
10. Krakowka S, and Bigbee JW (1983). In vitro properties of diffuse cytoplasmic esterase-positive canine mononuclear leukocytes. Vet. Immunol. Immunopathol.5: 1–13.
11.
11. Krakowka S (1983). Natural killer (NK) cell activity in adult gnotobiotic dogs. Am. J. Vet. Res.44: 635–638.
12.
12. Krakowka S, and Bigbee JW (1986). Activation specificity of commonly employed mitogens for canine B- and T-lymphocytes. Vet. Immunol. Immunopathol.11: 281–289.
13.
13. Krakowka S, Bigbee JW, Bigbee JW, Bigbee JW, and Bigbee JW (1987). Immunosuppression by canine distemper virus: Modulation of in vitro immunoglobulin synthesis, interleukin release and prostaglandin E2 production.Vet. Immunol. Immunopathol. (in press).
14.
14. Miller K (1985). Review: Immunotoxicology. Clin. Exp. Immunol.61: 219–223.
15.
15. Norbury KC (1982). Immunotoxicology in the pharmaceutical industry. Environ. Health Pers.43: 53–59.
16.
16. Recalde HR (1984). A simple method of obtaining monocytes in suspension. J. Immunol. Methods69: 71–79.
17.
17. Ringler SS, and Bigbee JW (1985). Cell surface markers of the canine natural killer (NK) cell. Vet. Immunol. Immunopathol.9: 1–11.
18.
18. Tarr MK, Bigbee JW, and Bigbee JW (1983). Comparison of in vitro and in vivo immunotoxicology assays. Ann. New York Acad. Sci.407: 469–471.
19.
19. Vandevelde M, Bigbee JW, Bigbee JW, Bigbee JW, and Bigbee JW (1983). Histological immunoenzyme techniques in canine tissues: Evaluation of various methods and modifications. Res. Vet. Sci.34: 193–198.
