Elevated Lipid Peroxidation in Rats Induced by Dietary Lipids and N -Nitrosodimethylamine and its Inhibition by Indomethacin Monitored Via Ethane Exhalation
Free accessResearch articleFirst published online January, 1987
Elevated Lipid Peroxidation in Rats Induced by Dietary Lipids and N -Nitrosodimethylamine and its Inhibition by Indomethacin Monitored Via Ethane Exhalation
The effect of dietary lipids alone or in combination with an administered carcinogen, N-nitrosodimethylamine (NDMA), on whole body lipid peroxidation was studied in rats in vivo. Groups of rats were fed diets containing 2%, 12.5%, or 25% of either saturated or polyunsaturated fat. Lipid peroxidation in individual animals was determined by measuring the concentration of ethane in exhaled air. Increased ethane exhalation was found in rats when the amount of dietary fat was increased from 2% to 12.5%, but animals receiving 12.5% or 25% fat in the diet exhaled ethane at similar rates. Rats consuming polyunsaturated fat exhaled more ethane than those eating saturated fat. In all groups, NDMA administration drastically increased ethane exhalation. Indomethacin completely blocked the increase in ethane exhalation caused by dietary lipids.
References
1.
1. Abraham S and Hillyard LA (1983). Effect of dietary 18-carbon fatty acids on growth of transplantable mammary and adenocarcinomas in mice. J. Natl. Cancer Inst.71:601–605.
2.
2. Alvares AP, Anderson KE, Conney AH, and Kappas A (1976). Interactions between nutritional factors and drug biotransformations in man. Proc. Natl. Acad. Sci. USA73:2501–2504.
3.
3. Ames BN, Hollstein MC, and Catchart R (1982). Lipid peroxidation and oxidative damage to DNA. In: Lipid Peroxides in Biology and Medicine, S Yagi (ed). Academic Press, New York, pp. 339–351.
4.
4. Armstrong B and Doll R (1975). Environmental factors and cancer incidence and mortality in different countries, with special reference to dietary practices. Int. J. Cancer15:617–631.
5.
5. Bernheim F, Bernheim ML, and Wilbur KM (1948). The reaction between thiobarbituric acid and the oxidation products of certain lipids. J. Biol. Chem.174:257–264.
6.
6. Bull AW, Nigra ND, Golembieski WA, Crissman JD, and Marnett LJ (1984). In vivo stimulation of DNA synthesis and induction of ornithine decarboxylase in rat colon by fatty acid hydroperoxides, autooxidation products of unsaturated fatty acids. Cancer Res.44:4924–4928.
7.
7. Carroll KK (1980). Lipids and carcinogenesis. J. Environ. Pathol. Toxicol.3:253–271.
8.
8. Carroll KK and Davidson MB (1982). The role of lipids in tumorigenesis. In: Molecular Interrelations of Nutrition and Cancer, MS Amott, J Van Eys, and Y-M Wang (eds). Raven Press, New York, pp. 237–245.
9.
9. Carroll KK and Khor HT (1971). Effects of level and type of dietary fat on incidence of mammary tumors induced in female Sprague-Dawley rats by 7,12-dimethylbenz(α)anthracene. Lipids6:415–420.
10.
10. Carter CA, Milholl RJ, Shea W, and Ip MM (1983). Effect of prostaglandin synthetase inhibitor indo-methacin on 7,12-dimethylbenz(a)anthracene-induced mammary tumorigenesis in rats fed different levels of fat. Cancer Res.43:3559–3562.
11.
11. Cave WT and Jurkowski JJ (1984). Dietary lipid effects on the growth, membrane composition, and prolactin-binding capacity of rat mammary tumors. J. Natl. Cancer Inst.73:185–191.
12.
12. Cerutti PA (1985). Prooxidant states and tumor promotion. Science227:375–381.
13.
13. Cornwell DG and Morisaki N (1984). Fatty acid paradoxes in the control of cell proliferation: Prostaglandins, lipid peroxides, and cooxidation reactions. In: Free Radicals in Biology, Vol. VI, WA Pryor (ed). Academic Press, New York, pp. 95–148.
14.
14. Correa P (1981). Epidemiological correlations between diet and cancer frequency. Cancer Res.41:3685–3690.
15.
15. Demopoulos HB, Pietronigro DD, Flamm ES, and Seligman ML (1980). The possible role of free radical reactions in carcinogenesis. J. Environ. Pathol. Toxicol.3:273–303.
16.
16. Flower RJ, Moncada S, and Vane JR (1980). Analgesic-antipyretics and anti-inflammatory agents; drugs employed in the treatment of gout. In: The Pharmacological Basis of Therapeutics, 6th ed., AG Gilman, LS Goodman, and A Gilman (eds). Macmillan, New York, pp. 682–728.
17.
17. Gavino VC, Miller JS, Ikharebha SO, Milo GE, and Cornwell DG (1981). Effect of polyunsaturated fatty acids and antioxidants on lipid peroxidation in tissue cultures. J. Lipid Res.22:763–769.
18.
18. Gupta I, Suzuki K, Bruce WR, Krepinsky JJ, and Yates P (1984). A model study of fecapentaenes: Mutagens of bacterial origin with alkylating properties. Science225:521–523.
19.
19. Hildebrandt AG, Bergs C, Heinemeyer G, Schlede E, Roots J, Abbas-Ali, P, and Schmoldt A (1982). Studies on the mechanism of stimulation of microsomal H2O2 formation and benzo(a)pyrene hydroxylation by substrates and flavone. Adv. Exp. Med. Bio.136A: 179–198.
20.
20. Hillyard LA and Abraham S (1979). Effect of dietary polyunsaturated fatty acids on growth of mammary adenocarcinomas in mice and rats. Cancer Res.39:4430–4437.
21.
21. Hirai N, Kingston DGI, Van Tassel RL, and Wilkins TD (1982). Structure elucidation of a potent mutagen from human feces. J. Am. Chem. Soc.104:6149–6150.
22.
22. Lands WEM, Kulmacz RJ, and Marshall PJ (1984). Lipid peroxide actions in the regulation of prostaglandin biosynthesis. In: Free Radicals in Biology, Vol. VI, WA Pryor (ed). Academic Press, New York, pp. 39–61.
23.
23. Lawrence GD and Cohen G (1984). Concentrating ethane from breath to monitor lipid peroxidation in vivo. Meth. Enzymol105: 305–311.
24.
24. Lijinksy W and Reuber MD (1984). Carcinogenesis in rats by nitrosomethylamine and other nitrosomethylalkylaminesat low doses. Cancer Lett.22:83–88.
25.
25. Miller JA, Kline BE, Rush HP, and Baumann CA (1944). The effect of certain lipids on the carcinogenicity of p-dimethylaminoazobenzene. Cancer Res.4:756–761.
26.
26. Morisaki N, Stitts JM, Bartels-Tomei, L, Milo GE, Panganamala RV, and Cornwell DG (1982). Dipyridamole: An antioxidant that promotes the proliferation of aorta smooth muscle cells. Artery11:88–107.
27.
27. Smith MT, Thor H, Hartzell P, and Orrenius S (1982). The measurement of lipid peroxidation in isolated hepatocytes. Biochem. Pharmacol.31:19–26.
28.
28. Tannenbaum A (1959). Nutrition and cancer. In: The Physiopathology of Cancer, 2nd ed., F Homburger (ed). Hoeber-Harper, New York, pp. 517–562.
29.
29. Totter JR (1980). Spontaneous cancer and its possible relationship to oxygen metabolism. Proc. Natl. Acad. Sci. USA77:1763–1767.
30.
30. Watson AF and Mellanby E (1930). Tar cancer in mice. II: The condition of the skin when modified by external treatment or diet, as a factor in influencing the cancerous reaction. Br. J. Exp. Pathol.11:311–322.
31.
31. Wendel A and Dumelin EE (1981). Hydrocarbon exhalation. Meth. Enzymol.11:10–15.
