The influence of Hb on 14 serum constituents was evaluated. Among the 7 enzymes for which analyses were conducted, LD and HBD values were elevated fivefold at the highest Hb concentration of 1500 mg/liter. Hb had less of an effect on the activities of other enzymes, i.e., AST activity was increased by only 30% whereas CK activity was decreased by 10%. Among the 7 nonenzymatic constituents of rat serum studied, a Hb concentration of 1500 mg/liter resulted in a fivefold increase in total bilirubin concentration. Creatinine and glucose values were negatively correlated to Hb concentrations but the dependence was not nearly as great as that with total bilirubin. The remaining serum constituents studied were not affected by Hb concentrations.
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