Extraction and purification of total flavonoids from cannabis fiber extracts were studied by using ethyl acetate and three different resins. The results indicated that the HPD-500 resin was appropriate and its adsorption data were well fitted to pseudo-second-order kinetics model. Purified with HPD-500, 8.36% purity of flavonoids can be obtained. The chemical structure showed that the flavonoid extracts had C=C, C-H, C-O and rutin derivatives. The thermal decomposition of Cannabis sativa fiber extracts can be divided into four stages, which was in agreement with the thermal decomposition properties of flavonoids in the literature. The shielding effect of polyvinyl alcohol (PVA) on ultraviolet-visible light was enhanced by the addition of flavonoid extracts, and the transmittance decreased from 82.89% to 59.12%. The results of antioxidant activities of the HPD-500 purified flavonoids showed that ·DPPH radical scavenging increased from 10.18% to 17.19% and ·O2− increased from 5.09% to 8.97% correspondingly. The results of antibacterial testing showed that the antibacterial activities of PVA with purified flavonoids were 63% higher than that of PVA without flavonoids. This indicated that the purified flavonoids from hemp fibers can be used as a source of potential antioxidant and antibacterial agents.
KornpointnerCSainz MartinezAMarinovicS, et al.,
Chemical composition and antioxidant potential of Cannabis sativa L. roots. Ind Crop Prod2021; 165: 113422.
2.
TurnerCEElsohlyMABoerenEG.Constituents of Cannabis sativa L. XVII. A review of the natural constituents. J Nat Prod1980;
43: 169–234.
3.
RossSAElSohlyMASultanaGN, et al.,
Flavonoid glycosides and cannabinoids from the pollen of Cannabis sativa L.Phytochem Anal2005;
16: 45–48.
4.
ElsohlyMASladeD.Chemical constituents of marijuana: the complex mixture of natural cannabinoids. Life Sci2005;
78: 539–548.
5.
ElSohlyMAGulW. Constituents of Cannabis sativa. In Eric Murillo-Rodriguez, Pandi-Perumal SR, Jaime MM (eds) Handbook of cannabis. Oxford: Oxford University Press, 2014.
6.
RussoEBMarcuJ.Cannabis pharmacology: the usual suspects and a few promising leads. Adv Pharmacol2017;
80: 67–134.
7.
PollastroFMinassiAFresuLG.Cannabis phenolics and their bioactivities. Curr Med Chem2018;
25: 1160–1185.
8.
JinDDaiKXieZ, et al.,
Secondary metabolites profiled in cannabis inflorescences, leaves, stem barks, and roots for medicinal purposes. Sci Rep2020;
10: 3309.
9.
Rice-EvansCAMillerNJPagangaG.Structure-antioxidant activity relationships of flavonoids and phenolic acids.Free Rad Biol Med1996;
20: 933–956.
10.
GaoSHYuCW.Supercritical carbon dioxide extraction and bacterial resistance of flavones from apocynum venetum bast fiber. J Text Res2018;
39: 71–76.
11.
CuiQLiJYuC.Optimization and characterization of flavonoids extracted from Cannabis sativa fibers. Text Res J 2021,7: 00405175211027796.
12.
LiJChaseHA.Development of adsorptive (non-ionic) macroporous resins and their uses in the purification of pharmacologically-active natural products from plant sources. Nat Prod Rep2010;
27: 1493–510.
13.
JinXLiuMChenZ, et al.,
Separation and purification of epigallocatechin-3-gallate (EGCG) from green tea using combined macroporous resin and polyamide column chromatography. J Chromatogr B Anal Technol Biomed Life Sci2015;
1002: 113–122.
14.
QuLXinHSuY, et al.
Combined application of macroporous resin and high speed counter-current chromatography for preparative separation of three flavonoid triglycosides from the leaves of Actinidia valvata Dunn. J Sep Sci2012;
35: 883–892.
15.
HuangPZhangQPanH, et al.
Optimization of integrated extraction-adsorption process for the extraction and purification of total flavonoids from Scutellariae barbatae herba.Sep Purif Technol2017;
175: 203–212.
16.
WanPShengZHanQ, et al.
Enrichment and purification of total flavonoids from Flos Populi extracts with macroporous resins and evaluation of antioxidant activities in vitro. J Chromatogr B Anal Technol Biomed Life Sci2014;
945-946: 68–74.
17.
RenJZhengYLinZ, et al.
Macroporous resin purification and characterization of flavonoids from Platycladus orientalis (L.) Franco and their effects on macrophage inflammatory response. Food Funct2017;
8: 86–95.
18.
DongYZhaoMSun-WaterhouseD, et al.
Absorption and desorption behaviour of the flavonoids from Glycyrrhiza glabra L. leaf on macroporous adsorption resins. Food Chem2015;
168: 538–545.
19.
Shekhar Sharma HS and Van Sumere CF. The biology and processing of flax. Northern Ireland: Belfast Publications, Publications, 1992.
20.
Konczewicz W and Kozłowski RM. Enzymatic treatment of natural fibres. In Ryszard M. Kozłowski (ed) Handbook of natural fibres. Cambridge: Woodhead Publishing, 2012, pp.168–184.
21.
AllamA.Assessment, evaluation and acknowledgment of a new, more controllable method for extracting vegetal fibers. J Nat Fibers2005;
1: 77–85.
22.
WangHMPostleRKesslerRW, et al.
Removing pectin and lignin during chemical processing of hemp for textile applications. Text Res J2016;
73: 664–669.
23.
ZhangJZhangJ.Effect of refined processing on the physical and chemical properties of hemp bast fibers. Text Res J2009;
80: 744–753.
24.
HouMHuWXiuZ, et al.
Efficient enrichment of total flavonoids from Pteris ensiformis Burm. extracts by macroporous adsorption resins and in vitro evaluation of antioxidant and antiproliferative activities. J Chromatogr B Anal Technol Biomed Life Sci2020;
1138: 121960.
25.
RothenburgerSSpanglerDBhendeS, et al.
In vitro antimicrobial evaluation of Coated VICRYL* Plus Antibacterial Suture (coated polyglactin 910 with triclosan) using zone of inhibition assays. Surg Infect (Larchmt)2002;
3Suppl 1: S79–87.
26.
ChenXHouDWangL, et al.
Antibacterial surgical silk sutures using a high-performance slow-release carrier coating system. ACS Appl Mater Interfaces2015;
7: 22394–22403.
27.
HanHZhuJWuDQ, et al.
Inherent guanidine nanogels with durable antibacterial and bacterially antiadhesive properties. Adv Funct Mater2019;
29: 1806594.
28.
NorooziBSorialGABahramiH, et al.
Equilibrium and kinetic adsorption study of a cationic dye by a natural adsorbent–silkworm pupa. J Hazard Mater2007;
139: 167–174.
29.
SyafiuddinAFulazzakyMASalmiatiS, et al.
Silver nanoparticles adsorption by the synthetic and natural adsorbent materials: an exclusive review. Nanotechnol Environ Eng2020; 5: 1–18.
30.
NikmatinSSyafiuddinAKuehA, et al.
Effects of nanoparticle filler on thermo-physical properties of rattan powder-filled polypropylene composites. Jurnal Teknologi 2015; 77: 181–187.
31.
LinZ.Purification and structural characterization of flavonoids and polysaccharides from platycladus orientalis (l.) franco leaves and their biological activities. Master’s Thesis,
South China University of Technology,
Guang-dong, 2016.
