Factors affecting the mechanical and geometrical properties of electrostatically flocked pure chitosan fiber scaffolds

Abstract

The field of articular cartilage tissue engineering has developed rapidly, and chitosan has become a promising material for scaffold fabrication. For this paper, wet-spun biocompatible chitosan filament yarns were converted into short flock fibers and subsequently electrostatically flocked onto a chitosan substrate, resulting in a pure, highly open, porous, and biodegradable chitosan scaffold. Analyzing the wet-spinning of chitosan revealed its advantages and disadvantages with respect to the fabrication of the fiber-based chitosan scaffolds. The scaffolds were prepared using varying processing parameters and were analyzed in regards to their geometrical and mechanical properties. It was found that the pore sizes were adjustable between 65 and 310 µm, and the compressive strength was in the range 13–57 kPa.

Keywords

chitosan fiber wet spinning electrostatic flocking mechanical properties flock scaffold

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References

Nicolini

Carvalho

Dragone

et al.

Updates in biological therapies for knee injuries: full thickness cartilage defect. Curr Rev Musculoskelet Med 2014; 7: 256–262.

Makris

Gomoll

Malizos

et al.

Repair and tissue engineering techniques for articular cartilage. Nat Rev Rheumatol 2015; 11: 21–34.

Hunziker

Lippuner

Keel

et al.

An educational review of cartilage repair: precepts & practice – myths & misconceptions – progress & prospects. Osteoarthr Cartil 2015; 23: 334–350.

Johnstone

Alini

Cucchiarini

et al.

Tissue engineering for articular cartilage repair: the state of the art. Eur Cell Mater 2013; 25: 248–267.

Hutmacher

. Scaffolds in tissue engineering bone and cartilage. Biomaterials 2000; 21: 2529–2543.

Gunn

Zhang

. Polyblend nanofibers for biomedical applications: perspectives and challenges. Trends Biotechnol 2010; 28: 189–197.

Levengood

Zhang

. Chitosan-based scaffolds for bone tissue engineering. J Mater Chem B 2014; 2: 3161–3184.

Younes

Rinaudo

. Chitin and chitosan preparation from marine sources: structure, properties and applications. Mar Drugs 2015; 13: 1133–1174.

Croisier

Jérôme

. Chitosan-based biomaterials for tissue engineering. Eur Polym J 2013; 49: 780–792.

10.

Suh

Matthew

. Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering: a review. Biomaterials 2000; 21: 2589–2598.

11.

Loh

Choong

. Three-dimensional scaffolds for tissue engineering applications: role of porosity and pore size. Tissue Eng Part B Rev 2013; 19: 485–502.

12.

Talebian

Mehrali

Mohan

et al.

Chitosan (PEO)/bioactive glass hybrid nanofibers for bone tissue engineering. RSC Advances 2014; 4: 49144–49152.

13.

Khanlou

Ang

Talebian

et al.

Electrospinning of polymethyl methacrylate nanofibers: optimization of processing parameters using the Taguchi design of experiments. Text Res J 2014; 85: 4356–4368.

14.

Talebian

Afifi

Khanlou

. Fabrication and characterisation of chitosan/poly vinyl alcohol nanofibres via electrospinning. Mater Res Innov 2014; 18: 331–335.

15.

Talebian S, Afifi AM, Hatami M, et al. Preparation and characterisation of electrospun silica nanofibres. Mater Res Innov 2014; 18: 510–514.

16.

Zhao Y, Qiu Y, Wang W, et al. Preparation of nanofibers with renewable polymers and their application in wound dressing. Int J Polym Sci 2016; Article 4672839.

17.

Tamayol

Akbari

Annabi

et al.

Fiber-based tissue engineering: progress, challenges, and opportunities. Biotechnol Adv 2013; 31: 669–687.

18.

Aibibu

Hild

Wöltje

et al.

Textile cell-free scaffolds for in situ tissue engineering applications. J Mater Sci Mater Med 2016; 27: 1–20.

19.

Walther

Hoyer

Springer

et al.

Novel textile scaffolds generated by flock technology for tissue engineering of bone and cartilage. Materials 2012; 5: 540–557.

20.

Walther

Bernhardt

Pompe

et al.

Development of novel scaffolds for tissue engineering by flock technology. Text Res J 2007; 77: 892–899.

21.

Balasubramanian

Boccaccini

. Bilayered bioactive glass scaffolds incorporating fibrous morphology by flock technology. Mater Lett 2015; 158: 313–316.

22.

Vellayappan

Jaganathan

Supriyanto

. Review: unraveling the less explored flocking technology for tissue engineering scaffolds. RSC Advances 2015; 5: 73225–73240.

23.

Steck

Bertram

Walther

et al.

Enhanced biochemical and biomechanical properties of scaffolds generated by flock technology for cartilage tissue engineering. Tissue Eng Part A 2010; 16: 3697–3707.

24.

Gossla

Tonndorf

Bernhardt

et al.

Electrostatic flocking of chitosan fibers leads to highly porous, elastic and fully biodegradable anisotropic scaffolds. Acta Biomater 2016; 44: 267–276.

25.

Liang

. Influence of molecular parameters on the degradation of chitosan by a commercial enzyme. Polym Degrad Stab 2007; 92: 515–524.

26.

Prasitsilp

Jenwithisuk

Kongsuwan

et al.

Cellular responses to chitosan in vitro: the importance of deacetylation. J Mater Sci Mater Med 2000; 11: 773–778.

27.

Toskas

Brünler

Hund

et al.

Pure chitosan microfibres for biomedical applications. Autex Res J 2013; 13: 134–140.

28.

Hild

Toskas

Aibibu

et al.

Chitosan/gelatin micro/nanofiber 3D composite scaffolds for regenerative medicine. Compos Interface 2014; 21: 301–308.

29.

Shepherd

Seedhom

. Thickness of human articular cartilage in joints of the lower limb. Ann Rheum Dis 1999; 58: 27–34.

30.

Schindelin

Arganda-Carreras

Frise

et al.

Fiji: an open-source platform for biological-image analysis. Nat Methods 2012; 9: 676–682.

31.

Cooper

Laxer

Hansbrough

. The cytotoxic effects of commonly used topical antimicrobial agents on human fibroblasts and keratinocytes. J Trauma Acute Care Sur 1991; 31: 775–784.

32.

Lineaweaver

McMorris

Soucy

et al.

Cellular and bacterial toxicities of topical antimicrobials. Plast Reconstr Surg 1985; 75: 394–396.

33.

Knaul

Hudson

Creber

. Improved mechanical properties of chitosan fibers. J Appl Polym Sci 1999; 72: 1721–1732.

34.

Notin

Viton

David

et al.

Morphology and mechanical properties of chitosan fibers obtained by gel-spinning: influence of the dry-jet-stretching step and ageing. Acta Biomater 2006; 2: 387–402.

35.

Kerin

Wisnom

Adams

. The compressive strength of articular cartilage. Proc Inst Mech Eng H J Eng Med 1998; 212: 273–280.

36.

Aibibu

Hild

Cherif

An overview of braiding structure in medical textile: fiber-based implants and tissue engineering. In: Kyosev

(ed). Advances in braiding technology: Specialized techniques and applications, Cambridge: Woodhead Publishing, 2016.

37.

Min

Lee

Lim

et al.

Chitin and chitosan nanofibers: electrospinning of chitin and deacetylation of chitin nanofibers. Polymer 2004; 45: 7137–7142.

38.

Geng

Kwon

Jang

. Electrospinning of chitosan dissolved in concentrated acetic acid solution. Biomaterials 2005; 26: 5427–5432.

39.

Haider

Park

. Preparation of the electrospun chitosan nanofibers and their applications to the adsorption of Cu (II) and Pb (II) ions from an aqueous solution. J Membr Sci 2009; 328: 90–96.

40.

Homayoni

Ravandi

Valizadeh

. Electrospinning of chitosan nanofibers: processing optimization. Carbohydr Polym 2009; 77: 656–661.

41.

Sun

Long

Zhang

et al.

Advances in three-dimensional nanofibrous macrostructures via electrospinning. Prog Polym Sci 2014; 39: 862–890.

42.

Ucar

Yilgor

Hasirci

et al.

Chitosan-based wet-spun scaffolds for bioactive agent delivery. J Appl Polym Sci 2013; 130: 3759–3769.

43.

Jana

Florczyk

Leung

et al.

High-strength pure porous chitosan scaffolds for tissue engineering. J Mater Chem 2012; 22: 6291–6299.

44.

Wang

Romer

Connell

et al.

Highly flexible silica/chitosan hybrid scaffolds with oriented pores for tissue regeneration. J Mater Chem B 2015; 3: 7560–7576.

45.

Wan

Cao

et al.

Compressive mechanical properties and biodegradability of porous poly(caprolactone)/chitosan scaffolds. Polym Degrad Stabil 2008; 93: 1736–1741.

46.

Pourhaghgouy

Zamanian

. Physical and mechanical properties of the fully interconnected chitosan ice-templated scaffolds. J Appl Polym Sci 2015; 132: 41476–41476. doi: 10.1002/app.41476.