Extraction and characterization of pigment from Sclerotinia sp. and its use in dyeing cotton

Abstract

The fruiting body of a cup fungus was collected from a compost pit containing fresh cow dung amended with biodynamic herbal preparations. The fungus was identified as Sclerotinia sp. by following standard procedures. The mycelial growth of Sclerotinia sp. on cow pat pit manure was monitored for 90 days and on day 5 pin head primordia were observed and matured into individual fruiting bodies (0.5 cm diameter each, 1 gram fresh weight) on day 20. The fruiting body started yielding dark green spores and the spore deposits were seen on the top surface of the fruiting body. The spores are liberated from the fruiting bodies when they receive an external stimulus. The Sclerotinia sp. spore was elliptical in shape and was 2 µm in size when examined under phase contrast microscope at 40x. The dark green spore of Sclerotinia sp. excretes a pinkish red pigment in distilled water (pH 6.5). Spectrophotometer scanning (200—800 nm) of the pigment showed three peaks: 507 nm (optical density (O.D.) 0.554), 525 nm (O.D. 0.629), and 545 nm (O.D. 0.567). The pigment had high protein content (0.332 mg/ml) but no deductible amount of carbohydrates or lipids. Stability of the pigment was tested at various temperatures (40—100°C) and no change in its original color was observed under UV—visible spectral scan analysis. Pigment from Sclerotinia sp. changed its original pinkish red color at various pH values (pH 2.0, 3.0, and 14.0). The antibacterial activity of this pigment extract was tested on seven different bacteria of which a zone of inhibition was observed for E. coli. The pigment from Sclerotinia sp. applied on cotton yarns pretreated with different mordants gave different color shades, which were confirmed using the standard color code index.

Keywords

Sclerotinia sp.pigments cow pat pit manure antimicrobial activity cotton yarn dyeing

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References

Gulrajani, M.L. , Present Status of Natural Dyes, In "Convention on Natural dyes ", Department of Textile Technology, IIT Delhi, 2002, pp. 1-10.

Bechtold, T. , Turcanu, A. , Ganglberger, E. , and Geissler, S. , Natural Dyes in Modern Textile Dyehouses - How to Combine Experiences of Two Centuries to Meet the Demands of the Future? , J. Cleaner Production, 11(5), 499-509 (2003).

Chavan, A.M. , Effect of Fungal Pigment on Hydrolytic Enzyme Activity, Flora Fauna, 2(1), 29-30 (1996).

Perumal, K. , "Studies on the Microbial Production of Natural Dyes and Application in Paper Products", DST/SSD/SP/YO/ 004/2001 Project Report, Department of Science and Technology, New Delhi, 2006.

Pernezny, K. , and Purdy, L.H. , "Sclerotinia Diseases of Vegetable and Field Crops in Florida", PP22, Plant Pathology Department, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida , 1994, available online at http://edis.ifas.ufl.edu/pdffiles/VH/VH01500.pdf

Senthilkumar, M. , Gurumoorthi, P. , and Janardhanan K. , Antibacterial Potential of Some Plants Used by Tribals in Maruthamalai Hills, Tamil Nadu, Natural Prod. Radiance, 4(1), 27-34 (2005).

Subramanian, C.V. , "Hypomycetes, An Account of Indian species Except Cercosporae", Indian Council of Agricultural Research , New Delhi, 1971.

Alexopolus, C.J. , and Mims, C.W. , "Introductory Mycology", 3rd edn, John Wiley & Sons, 1979, p. 368.

Carvalho, J.C. , Baggio, C.B. , Karb, S.G. , Miyaoka, M. , Soccol, C.R. , Comparison of Different Solvent Efficiencies on the Extraction of Pigments Produced by Monascus sp. in Solid State Fermentation , In: VII SHEB Maringa Anais VII SHEB, 1, 229 (2002).

10.

Lowry, O.H. , Rosebrough, N.J. , Farr, A.L. , and Randall, R.J. , Protein Measurement with the Folin-Phenol Reagent, J. Biol. Chem., 193, 265-275 ( 1951).

11.

Sadasivam, S. , and Manickam, A. , "Biochemical Methods", 2nd edn, New Age International (P) Limited, 1996, pp. 1-5, 33-34.

12.

Erturk, O. , Kati, H. , Yayli, N. , and Demirbag, Z. , Antimicrobial Activity of Viscum album subsp. Abietis, Turk. J. Biol., 27(4), 255-258 (2003).

13.

Srivastava, R.C. , Goel, M. , and Gulrajani, M.L. , Color Gamut of Natural Dyes on Cotton Yarns , in "Convention on Natural Dyes", Department of Textile Technology, IIT Delhi, 1999, pp. 288-296.

14.

Perumal, K. , Stanley Abraham, L. , and Vatsala, T.M. , Isolation, Extraction and Dyeing Performance of Fungal Pigment from Ganoderma lucidum, Coriolus versicolor and Amanita muscaria, J. Mycol. Pl. Pathol,. 34(2), 214-217 (2004).

15.

Moses, J.J. , Venkatachalam, A. , and Ramachandran, T. , Application of Some Natural Dyes on Jute/Cotton Fabric, in "Convention on Natural Dyes", Department of Textile Technology, IIT Delhi, 1999, pp. 190-204.

16.

Yongsmith, B. , Krairak, S. , and Bavavoda, R. , Production of Yellow Pigments in Submerged Culture of a Mutant of Monascus sp., J. Ferment. Bioeng., 78, 223-228 (1994).

17.

Hajjaj, H. , Blance, P. , Groussac, E. , Uribelarrea, J.L. , Goma, G. , and Loubiere, P. , Kinetic Analysis of Red Pigment and Citrinin by Monascus Rubber as a Function of Organic Acid Accumulation, Enzyme Microbial. Technol., 27, 619-625 (2000).

18.

Williams, R.P. , Gott, C.L. , Qadri, S.M.H. , and Scott, R.H. , Influence of Temperature of Incubation and Type of Growth Medium on Pigmentation in Serratia marcescens, J. Bacteriol., 106, 438-443 (1971).

19.

Kim, C.M. , Kim, S.W. , and Hong, S.I. , Production of Red Pigment by Serratia sp. KH-95 and its Cultural Properties, Korean J. Biotechnol. Bioeng. , 13, 431-437 (1998).

20.

Kim, C.M. , Kim, S.W. , and Hong, S.I. , An Integrated Fermentation Separation Process for the Production of Red Pigment by Serratia sp. KH-95, Process Biochem., 35, 485-490 (1999).

21.

Oshima, M. , Ishizaki, N. , and Handa, A. , Neopurpuratin is a Purplish-Red Pigment Containing Ferrous Ion, Produced by Streptomyces Propurpuratus ATCC 21630 in Cooperation with Bacillus sp. under Mixed Culture, J. Ferment. Technol., 59, 209-212 ( 1981).

22.

Rye, B.H. , Park, B.G. , Chi, Y.E. , and Lee, J.H. , Production of Purplish-Red Pigment in Mixed Culture of Streptomyces Propurpuratus ATCC 21630 and Bacillus sp. R-89, Korean J. Appl. Microbiol. Bioeng., 17, 327-333 ( 1989).

23.

Fellows, P. , "Food Processing Technology - Principles and Practices", Ellis Horwood Ltd, Chichester, England, 1988, pp. 33-68.

24.

Francis, F.J. , "Lesser Known Food Colorants", Food Technology, 1987, pp. 41-64.

25.

Pandey, A. , and Babitha, S. , Microbial Pigments, Advanced Biotech., 3(8), 17-19 (2005).

26.

Lewis, M.J. , and Johnson, E.A. , Astaxanthin Formation by the Yeast Phaffia rhodozyma, J. Gen Microbiol. 115, 173-183 ( 1979).

27.

Sardaryan, E. , Zihlova, H. , Strnad, R. , and Cermakova, Z. , ArpinkRed - Meet a New Natural Red Food Colorant of Microbial Origin, in "Pigments in Food, More than Colours…", Dufossé, L. (ed.), Université de Bretagne Occidentale Publ., Quimper, France, 2004, pp. 207-208.

28.

Raisanen, R. , Bjork, H. , and Hynninen, P.H. , Two Dimensional TLC Separation and Mass Spectrophometric Identification of Anthraquinones Isolated from the Fungus Dermocybe sanguinea, Z. Naturforsch., 55, 192-202 ( 2000).

29.

Han, J.R. , Sclerotia Growth and Carotenoids Production of Penicillum sp. PT95 during Solid State Fermentation of Corn Meal, Biotechnol . Lett., 20(11), 1063-1065 ( 1998).