A rapid dehydration procedure with acidified 2,2-dimethoxypropane (DMP) has been successfully applied to immature or wet cotton fibers. The ultrastructural integrity and fiber quality parameters such as cell wall area and perimeter of developing and mature cotton fibers were well-pre-served using this procedure as demonstrated by light, scanning and electron microscopy of whole fibers and cross-sections. Embedding in resin following DMP dehydration also prevented the collapse of the immature fibers due to drying of lumen.
Bailey, Jr, T. L. W. , and Rollins, M. L. , Some Applications of Microscopy to Cotton Research , Textile Res. J.15, 1-6 (1945).
2.
Boylston, E. K. , Enhanced Preparation of Cotton Cross-sections for Image Analysis , “Proceedings of the Beltwide Cotton Conference, 1995,”National Cotton Council of America, Memphis, TN, pp. 1289-1290 , (1995).
3.
Boylston, E. K. , Evans, J. P. , and Thibodeaux, D. P. , A Quick Embedding Method for Light Microscopy and Image Analysis of Cotton Fibers , Biotechnic. & Histochem.70, 24-27 (1995).
4.
Tripp, V. W. , Some Applications of Light Microscopy in the Study of Textile Materials , Am. Soc. Testing Mats., Spec. Tech. Pub.143, 25-34 (1952).
5.
Goynes, W. R. , Ingber, B. F. , and Triplett, B. A. , Cotton Fiber Secondary Wall Development-time versus Thickness , Textile Res. J.65, 400-408 (1995).
6.
Backe, E. E. , “Cotton Moisture: Harvesting through Textile Processing,”Institute of Textile Technology, Charlottesville, VA , 2002.
7.
Jacks, T. J. , Muller, L. L. , and Evans, W. J. , Time Requirement for Chemical Dehydration of Tissues with 2,2-Dimethoxypro-pane for Microscopy , Microscope29, 137-140 (1981).
8.
Muller, L. L. , and Jacks, T. J. , Rapid Chemical Dehydration of Samples for Electron Microscopic Examinations , J. Histochem. Cytochem.23, 107-110 (1975).
9.
Newman, S. B. , Borysko, E. , and Swerdlow, M. , New Sectioning Techniques for Light and Electron Microscopy , Science110, 66-68 (1949).
10.
Spurr, A. R. , A Low-viscosity Epoxy Resin Embedding Medium for Electron Microscopy , J. Ultrastruct. Res.26, 31-43 (1969).
11.
Rajasekaran, K. , and Thibodeaux, D. P. , Fidelity of Cotton Fiber Maturity Measurement by Image Analysis , J. New Seeds4(3), 25-36 (2002).
12.
Yatsu, L. Y. , and Jacks, T. J. , Fixation of Cotton (Gossypium hirsutum L.) Hair Cells (fibers) . Microscope27, 113-116 (1979).
13.
Yatsu, L. Y. , and Jacks, T. J. , An Ultrastructural Study of the Relationship between Microtubules and Microfibrils in Cotton (Gossypium hirsutum L.) Cell Wall Reversals . Am. J. Bot. 68, 771-777 (1981).
14.
Boylston, E. K. , Hinojosa, O. , and Hebert, J. J. , A Quick Embedding Method for Light and Electron Microscopy of Textile Fibers , Biotechnic. & Histochem.66, 122-124 (1991).
15.
Thibodeaux, D. and Rajasekaran, K. , Development of New Reference Standards for Cotton Fiber Maturity , J. Cotton Sci.3, 188-193 (1999). http://www.jcotsci.org/1999/issue04/xNote/art01/article.pdf
16.
Nemanic, M. , Critical Point Drying, Cryofracture and Serial Sectioning, in “Scanning Electron Microscopy,”IITRI, Chicago, IL , pp. 297-304, 1972.
17.
Reynolds, E. S. , The Use of Lead Citrate at High pH as an Electron Opaque Stain in Electron Microscopy , J. Cell Biol.17, 208-212 (1963).
