Age estimation is a vital aspect of the process of identification. Studying the appearance and fusion of long bones is one of the most commonly used methods for age estimation. Most research conducted on age estimation using the study of the appearance and fusion of ossification centres has been roentgenographic in nature. However, X-ray examination and computed tomography examination are associated with ionisation radiation. The present study investigated the use of ultrasonography (USG) as a means of visualising ossification centres of the elbow and wrist joints for age estimation in 31 Maharashtrian boys from Ahmednagar, India. The Schmeling et al. method of grading was used to score the fusion of ossification centres, and simple and multiple linear regression models were developed for age estimation. It was found that the ossification centres of the elbow and wrist joints followed a set pattern of maturation and fusion. The ossification centres of the elbow joint fused before the ossification centres of the wrist joint. The fusion scores of proximal radial epiphyses had the highest correlation with the decimal age of the participants, making its fusion the best indicator among all the ossification centres examined in this study for age estimation. Regression models to estimate age were generated using all the ossification centres. USG was found to be suitable for the purpose of age estimation based on ease of examination, minimal ionisation risks, its non-invasive nature and clear visualisation of ossification centres.
KrogmanWMİşcanMY.The human skeleton in forensic medicine. 2nd ed.
Springfield, IL:
Charles C. Thomas, 1986.
9.
BlackSMAggrawalAPayne-JamesJ.Age estimation in the living : the practitioners guide.
Hoboken, NJ:
John Wiley, 2011.
10.
FranklinDFlavelANobleJ, et al.
Forensic age estimation in living individuals: methodological considerations in the context of medico-legal practice. Res Rep Forensic Med Sci2015;
53: 53–66.
11.
İşcanMYSteynM.The human skeleton in forensic medicine. 3rd ed.
Springfield, IL:
Charles C. Thomas, 2013.
12.
MartinDDWitJMHochbergZ, et al.
The use of bone age in clinical practice – part 1.Horm Res Paediatr2011;
76: 1–9.
13.
HellmanM.Ossification of epiphysial cartilages in the hand. Am J Phys Anthropol1928;
11: 223–257.
14.
BassedRBDrummerOHBriggsC, et al.
Age estimation and the medial clavicular epiphysis: analysis of the age of majority in an Australian population using computed tomography.Forensic Sci Med Pathol2011;
7: 148–154.
15.
BassedRBBriggsCDrummerOH.Age estimation using CT imaging of the third molar tooth, the medial clavicular epiphysis, and the spheno-occipital synchondrosis: a multifactorial approach.Forensic Sci Int2011;
212: 273.e1–e5.
National Research Council. Health risks from exposure to low levels of ionizing radiation: BEIR VII Phase 2.
Washington, DC:
National Academies Press, 2006.
18.
BilgiliYHizelSKaraSA, et al.
Accuracy of skeletal age assessment in children from birth to 6 years of age with the ultrasonographic version of the Greulich–Pyle Atlas.J Ultrasound Med2003;
22: 683–690.
19.
KhanKMMillerBSHoggardE, et al.
Application of ultrasound for bone age estimation in clinical practice.J Pediatr2009;
154: 243–247.
20.
Castriota-ScanderbegADe MicheliV.Ultrasound of femoral head cartilage: a new method of assessing bone age.Skeletal Radiol1995;
24: 197–200.
21.
MentzelH-JVilserCEulensteinM, et al.
Assessment of skeletal age at the wrist in children with a new ultrasound device.Pediatr Radiol2005;
35: 429–433.
22.
SchulzRSchiborrMPfeifferH, et al.
Forensic age estimation in living subjects based on ultrasound examination of the ossification of the olecranon.J Forensic Leg Med2014;
22: 68–72.
23.
WagnerUADiedrichVSchmittO.Determination of skeletal maturity by ultrasound: a preliminary report.Skeletal Radiol1995;
24: 417–420.
SchmelingASchulzRReisingerW, et al.
Studies on the time frame for ossification of the medial clavicular epiphyseal cartilage in conventional radiography.Int J Legal Med2004;
118: 5–8.
26.
AggrawalA. Age estimation in the living: in matters civil and criminal. J Anat. Epub ahead of print 11 May 2009. DOI: 10.1111/j.1469-7580.2009.01048.x.
27.
De BoerHHBlauSDelabardeT, et al.
The role of forensic anthropology in disaster victim identification (DVI): recent developments and future prospects. Forensic Sci Res2019;
4: 303–315.
28.
Aynsley-GreenAColeTJCrawleyH, et al.
Medical, statistical, ethical and human rights considerations in the assessment of age in children and young people subject to immigration control.Br Med Bull2012;
102: 17–42.
29.
SchmelingABaumannUSchmidtS, et al.
Reference data for the Thiemann–Nitz method of assessing skeletal age for the purpose of forensic age estimation.Int J Legal Med2006;
120: 1–4.
30.
SchmidtSBaumannUSchulzR, et al.
Study of age dependence of epiphyseal ossification of the hand skeleton.Int J Legal Med2008;
122: 51–54.
31.
BaumannUSchulzRReisingerW, et al.
Reference study on the time frame for ossification of the distal radius and ulnar epiphyses on the hand radiograph.Forensic Sci Int2009;
191: 15–18.
32.
SchmidtSSchiborrMPfeifferH, et al.
Age dependence of epiphyseal ossification of the distal radius in ultrasound diagnostics.Int J Legal Med2013;
127: 831–838.
33.
SchulzRZwiesigkPSchiborrM, et al.
Ultrasound studies on the time course of clavicular ossification.Int J Legal Med2008;
122: 163–167.
34.
SchmidtSSchmelingAZwiesigkP, et al.
Ultrasound studies on age dependency of epiphyseal ossification of the distal radius. Scand J Forensic Sci2011;
17: 17–20.
35.
SahniDJitI.Time of fusion of epiphyses at the elbow and wrist joints in girls of northwest India. Forensic Sci Int1995;
74: 47–55.
36.
WanJZhaoYFengQ, et al.
Potential value of conventional ultrasound in estimation of bone age in patients from birth to near adulthood. Ultrasound Med Biol2019;
45: 2878–2886.
37.
IntronaFCampobassoCP.Biological vs legal age of living individuals. In: SchmittACunhaEPinheiroJ, eds. Forensic anthropology and medicine: complementary sciences from recovery to cause of death.
Totowa, NJ:
Humana Press, 2006.
