Estimation of time since death from electrolyte,glucose and calcium analysis of postmortem vitreous humour in semi-arid climate

Abstract

Estimating time since death (TSD) with fair accuracy from postmortem changes still remains an important but difficult task to be performed by every autopsy surgeon in medicolegal scenario. The aim of the present study was to estimate TSD from electrolyte analysis of postmortem vitreous humour collected from samples under semi-arid climate. Vitreous humour was collected from 210 dead bodies brought to University College of Medical Sciences and Guru Teg Bahadur Hospital, Delhi, during the period of November 2010 to April 2012. The vitreous samples were analysed for sodium, potassium, calcium, chloride and glucose. The results showed a significant positive relationship between TSD and potassium (r = 0.841, p = 0.000) and a weak negative relationship between TSD and sodium (r = −0.137, p = 0.048) and glucose (r = −0.241, p = 0.000), whereas no significant relationship could be established between TSD and calcium (r = 0.055, p = 0.429) and chloride (r = 0.075, p = 0.11).

Keywords

Time since death electrolyte levels postmortem vitreous humour semi-arid climate

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References

Eckert

The medicolegal autopsy. In: Tedeschi

Eckert

Tedeschi

(eds). Forensic medicine: physical trauma 1977; Vol 2, Philadelphia: WB Saunders Company, pp. 995–997.

Chaudhary

Veena

Tirpude

. Potassium concentration in vitreous humour in relation to time since death. J Forensic Med Toxicol 2007; 24: 26–30.

Madea

. Is there recent progress in the estimation of the postmortem interval by means of thanatochemistry. Forensic Sci Int 2005; 151: 139–149.

Coe

Postmortem chemistry of blood, cerebrospinal fluid, and vitreous humour. In: Tedeschi

Eckert

Tedeschi

(eds). Forensic medicine: physical trauma 1977; Vol 2, Philadelphia: WB Saunders Company, pp. 1033–1052.

DiMaio

. Topics in forensic pathology. Forensic pathology, 2nd ed. Boca Raton, FL: CRC Press, 2001, pp. 48–49.

Jakubowski

Luetzelschwab

Aebischer

. Stability of clinical chemistry parameter values in minipig serum under different storage conditions. Scand J Anim Sci (Suppl) 1998; 25: 197–204.

Mulla A. Role of vitreous humour biochemistry in forensic pathology. MS Thesis, University of Saskatchewan, Saskatchewan, Canada, 2005, 146pp.

Madea

Kreuser

Banaschak

. Postmortem biochemical examination of synovial fluid – a preliminary study. Forensic Sci Int 2001; 118: 29–35.

Balasooriya

St Hill

Williams

. The biochemistry of vitreous humour – a comparative study of the potassium, sodium and urate concentrations in the eyes at identical time intervals after death. Forensic Sci Int 1984; 26: 85–91.

10.

Tao

Luo

. Contents of vitreous humor of dead body with different postmortem intervals. Sichuan Da Xue Xue Bao Yi Xue Ban 2006; 37: 898–900, 927.

11.

Yogiraj V, Indumati V and Kodliwadmath MV. Study of vitreous humour electrolytes to assess the postmortem interval and cause of death, http://www.anilaggrawal.com/ij/vol_009_no_002/papers/paper001.html (2008, accessed 19 April 2012).

12.

Jashnani

Kale

Rupani

. Vitreous humor: biochemical constituents in estimation of postmortem interval. J Forensic Sci 2010; 55: 1523–1527.

13.

Tumram

Bardale

Dongre

. Postmortem analysis of synovial fluid and vitreous humour for determination of death interval: a comparative study. Forensic Sci Int 2011; 204: 186–190.

14.

Sturner

. The vitreous humour: postmortem potassium changes. Lancet 1963; 7285: 807–808.

15.

Rognum

Hauge

Yasaeter

. A new biochemical method for estimation of postmortem time. Forensic Sci Int 1991; 51: 139–146.

16.

Agrawal

Gupta

Bhasin

. Determination of the time of death by estimating potassium level in the cadaver vitreous humour. Indian J Ophthalmol 1983; 31: 528–531.

17.

Tagliaro

Bortolotti

Manetto

. Potassium concentration differences in the vitreous humour from the two eyes revisited by microanalysis with capillary electrophoresis. J Chromatogr A 2001; 27; 924: 493–498.

18.

Muñoz

Suárez-Peñaranda

Otero

. A new perspective in the estimation of postmortem interval (PMI) based on vitreous. J Forensic Sci 2001; 46: 209–214.

19.

Vishal G. Co-relation of changes in levels of vitreous potassium and ascorbic acid with postmortem interval. MD Thesis, Baba Farid University of Health Sciences, Faridkot, India, 2005, 131pp.

20.

Zhou

Zhang

. The determination of potassium concentration in vitreous humor by low pressure ion chromatography and its application in the estimation of postmortem interval. J Chromatogr B Analyt Technol Biomed Life Sci 2007; 852: 278–281.

21.

Bortolotti

Pascali

Davis

. Study of vitreous potassium correlation with time since death in the postmortem range from 2 to 110 hours using capillary ion analysis. Med Sci Law 2011; 51: 20–23.

22.

Rajinderjit

Vishal

. Role of vitreous potassium level in estimating postmortem interval and the factors affecting it. J Clin Diagnostic Res 2011; 5: 13–15.