The long-term effectiveness of chlorhexidine as a matrix metalloproteinase (MMP) inhibitor may be compromised when water is incompletely removed during dentin bonding. This study challenged this anti-bond degradation strategy by testing the null hypothesis that wet-bonding with water or ethanol has no effect on the effectiveness of chlorhexidine in preventing hybrid layer degradation over an 18-month period. Acid-etched dentin was bonded under pulpal pressure simulation with Scotchbond MP and Single Bond 2, with water wet-bonding or with a hydrophobic adhesive with ethanol wet-bonding, with or without pre-treatment with chlorhexidine diacetate (CHD). Resin-dentin beams were prepared for bond strength and TEM evaluation after 24 hrs and after aging in artificial saliva for 9 and 18 mos. Bonds made to ethanol-saturated dentin did not change over time with preservation of hybrid layer integrity. Bonds made to CHD pre-treated acid-etched dentin with commercial adhesives with water wet-bonding were preserved after 9 mos but not after 18 mos, with severe hybrid layer degradation. The results led to rejection of the null hypothesis and highlight the concept of biomimetic water replacement from the collagen intrafibrillar compartments as the ultimate goal in extending the longevity of resin-dentin bonds.
BeckerTDAgeeKAJoyceAPRueggebergFABorkeJLWallerJL. (2007). Infiltration/evaporation-induced shrinkage of demineralized dentin by solvated model adhesives. J Biomed Mater Res B Appl Biomater80:156-165.
2.
BlackburnRSHarveyAKettleLLManianAPPayneJDRussellSJ (2007). Sorption of chlorhexidine on cellulose: mechanism of binding and molecular recognition. J Phys Chem B111:8775-8784.
3.
BreschiLCammelliFVisintiniEMazzoniAVitaFCarrilhoM. (2009). Influence of chlorhexidine concentration on the durability of etch-and-rinse dentin bonds: a 12-month in vitro study. J Adhes Dent11:191-198.
4.
CameronILShortNJFullertonGD (2007). Verification of simple hydration/dehydration methods to characterize multiple water compartments on tendon type 1 collagen. Cell Biol Int31:531-539.
5.
CarrilhoMRCarvalhoRMde GoesMFdi HipolitoVGeraldeliSTayFR. (2007a). Chlorhexidine preserves dentin bond in vitro. J Dent Res86:90-94.
6.
CarrilhoMRGeraldeliSTayFde GoesMFCarvalhoRMTjäderhaneL. (2007b). In vivo preservation of the hybrid layer by chlorhexidine. J Dent Res86:529-533.
7.
CollinsMJNielsen-MarshCMHillerJSmithCIRobertsJPPrigodichRV. (2002). The survival of organic matter in bone: a review. Archaeometry44:383-394.
8.
FullertonGDNesEAmuraoMRahalAKrasnosselskaiaLCameronI (2006). An NMR method to characterize multiple water compartments on mammalian collagen. Cell Biol Int30:66-73.
9.
GendronRGrenierDSorsaTMayrandD (1999). Inhibition of the activities of matrix metalloproteinases 2, 8, and 9 by chlorhexidine. Clin Diagn Lab Immunol6:437-439.
10.
HeblingJPashleyDHTjäderhaneLTayFR (2005). Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo. J Dent Res84:741-746.
11.
HiraishiNYiuCKKingNMTayFRPashleyDH (2008). Chlorhexidine release and water sorption characteristics of chlorhexidine-incorporated hydrophobic/hydrophilic resins. Dent Mater24:1391-1399.
12.
HosakaKNishitaniYTagamiJYoshiyamaMBrackettWWAgeeKA. (2009). Durability of resin-dentin bonds to water- vs. ethanol-saturated dentin. J Dent Res88:146-151.
13.
ItoSHashimotoMWadgaonkarBSvizeroNCarvalhoRMYiuC. (2005). Effects of resin hydrophilicity on water sorption and changes in modulus of elasticity. Biomaterials26:6449-6459.
14.
JiangXJiangNZhangHLiuM (2007). Small organic molecular imprinted materials: their preparation and application. Anal Bioanal Chem389:355-368.
15.
KimJGuLBreschiLTjäderhaneLChoiKKPashleyDH. (2010). Implication of ethanol wet bonding in hybrid layer remineralization. J Dent Res89:575-580.
16.
KimJUchiyamaTCarrilhoMAgeeKAMazzoniABreschiL. (2010c). Chlorhexidine binding to mineralized versus demineralized dentin powder. Dent Mater26:771-778.
17.
KimYKMaiSMazzoniALiuYTezvergil-MutluayATakahashiK. (2010). Biomimetic remineralization as a progressive dehydration mechanism of collagen matrices—Implications in the aging of resin-dentin bonds. Acta Biomater6:3729-3739.
18.
MagneDWeissPBoulerJMLabouxODaculsiG (2001). Study of the maturation of the organic (type I collagen) and mineral (nonstoichiometric apatite) constituents of a calcified tissue (dentin) as a function of location: a Fourier transform infrared microspectroscopic investigation. J Bone Miner Res16:750-757.
19.
MazzoniAPashleyDHRuggeriAJrVitaFFalconiMDi LenardaR. (2008). Adhesion to chondroitinase ABC treated dentin. J Biomed Mater Res B Appl Biomater86:228-236.
20.
MilesCABurjanadzeTV (2001). Thermal stability of collagen fibers in ethylene glycol. Biophys J80:1480-1486.
21.
MilesCAAveryNCRodinVVBaileyAJ (2005). The increase in denaturation temperature following cross-linking of collagen is caused by dehydration of the fibres. J Mol Biol346:551-556.
22.
NishitaniYYoshiyamaMDonnellyAMAgeeKASwordJTayFR. (2006). Effects of resin hydrophilicity on dentin bond strength. J Dent Res85:1016-1021.
23.
PashleyDHTayFRYiuCHashimotoMBreschiLCarvalhoRM. (2004). Collagen degradation by host-derived enzymes during aging. J Dent Res83:216-221.
24.
PashleyDHTayFRCarvalhoRMRueggebergFAAgeeKACarrilhoM. (2007). From dry bonding to water-wet bonding to ethanol-wet bonding. A review of the interactions between dentin matrix and solvated resins using a macromodel of the hybrid layer. Am J Dent20:7-20.
25.
SadekFTPashleyDHNishitaniYCarrilhoMRDonnellyAFerrariM. (2008). Application of hydrophobic resin adhesives to acid-etched dentin with an alternative wet bonding technique. J Biomed Mater Res A84:19-29.
26.
SauroSWatsonTFMannocciFMiyakeKHuffmanBPTayFR. (2009). Two-photon laser confocal microscopy of micropermeability of resin-dentin bonds made with water or ethanol wet bonding. J Biomed Mater Res B Appl Biomater90:327-337.
27.
TayFRGwinnettJAWeiSH (1996). Micromorphological spectrum from overdrying to overwetting acid-conditioned dentin in water-free acetone-based, single-bottle primer/adhesives. Dent Mater12:236-244.
28.
TayFRMouldingKMPashleyDH (1999). Distribution of nanofillers from a simplified-step adhesive in acid-conditioned dentin. J Adhes Dent1:103-117.
29.
TayFRPashleyDHKapurRRCarrilhoMRHurYBGarrettLV. (2007). Bonding BisGMA to dentin—a proof of concept for hydrophobic dentin bonding. J Dent Res86:1034-1039.
30.
WangYSpencerPHagerCBohatyB (2006). Comparison of interfacial characteristics of adhesive bonding to superficial versus deep dentine using SEM and staining techniques. J Dent34:26-34.
31.
WehrliFWFernández-SearaMA (2005). Nuclear magnetic resonance studies of bone water. Ann Biomed Eng33:79-86.
32.
YeQParkJGToppEWangYMisraASpencerP (2008). In vitro performance of nano-heterogeneous dentin adhesive. J Dent Res87:829-833.
33.
ZhouJTanJChenLLiDTanY (2009). The incorporation of chlorhexidine in a two-step self-etching adhesive preserves dentin bond in vitro. J Dent37:807-812.
