It has been reported that the presence of a smear layer on dentinal substrates can compromise bonding. Typically, smear layers are removed by acidic agents that selectively extract calcium salts from dentin surfaces to leave a collagen-rich substrate. Acid-conditioned dentin (i.e., demineralized) is then primed and an adhesive agent applied. In the present study, we removed smear layers by "polishing" dentin specimens with a hydroxyapatite paste and ultrasonication. Bonding procedures were carried out by means of an aqueous solution of 20% 2-methacryloyloxyethyl phenyl phosphoric acid (phenyl-P) and 30% 2-hydroxyethyl methacrylate, referred to as 20P-30H, a "self-etching primer". The 20P-30H solution was applied to "intact" dentin (i.e., non-demineralized) for either 30 or 60 s. Control samples received no application (0 s) of the self-etching primer. Mean tensile bond strengths (10 MPa) were similar in both the 30-second- and 60-second-primed groups. The widths of formed hybrid layers varied from 0.3 ± 0.2 pm at 0 s application (control) to 2.1 ± 0.3 μm for the 30-second group and 4.1 ± 0.2 pm for the 60-second group. SEM and TEM observations revealed that the 20P-30H self-etching primer created diffusion channels into "intact", calcium-rich dentin which permitted monomer to infiltrate dentin substrates. Hybrid layers identified under microscopic examination demonstrated resistance to both HCl and NaOCI treatments, suggesting that the hybrid layer was not defective, and that bonding was stable.
Amory C., Yvon J. ( 1994). Shear bond strength of a light-cured composite vs. dentin characteristics. Dent Mater10:203-209.
2.
Buonocore NG, Quigley N. (1958). Bonding of a synthetic resin material to human dentin: preliminary histological study of the bond area. J Am Dent Assoc57:807-811.
3.
Burrow NF, Tagami J., Hosoda H. (1993). The long term durability of bond strengths to dentin. Bull Tokyo Med Dent Univ40:173-191.
4.
Chigira H., Yukitani W., Hasegawa T., Manabe A., Itoh K., Hayakawa T., et al. (1994). Self-etching dentin primers containing phenyl-P. J Dent Res73:1088-1095.
Cobb DS, Vargas MA, Armstrong SR (1995). Shear bond strengths between acid-etched and deproteinized dentin surfaces (abstract). J Dent Res74(Spec Iss):35.
7.
Dickens S., Stangel I., Poulin S., Sacher E., Bowen RL (1994). Bond strength and surface analyses of unaltered and chemically-and non-chemically-altered dentin (abstract). J Dent Res73(Spec Iss):277.
8.
Eick JD, Cobb CM, Chappell RP, Spencer P., Robinson SJ (1991). The dentinal surface: Its influence on adhesion. Part 1. Quintessence Int22:967-977.
9.
Eick JD, Robinson SJ, Byerley TJ, Chappell RP, Spencer P., Chappelow CC (1995). Scanning transmission electron microscopy/energy dispersive spectroscopy analysis of the dentin adhesive interface using a labeled 2-hydroxyethyl methacrylate analogue. J Dent Res74:1246-1252.
10.
Eliades G., Palaghias G., Vougiouklakis G. (1990). Surface reactions of adhesives on dentin. Dent Mater6:208-216.
11.
Gwinnett AJ (1994). Chemically conditioned dentin. A comparison of conventional and environmental scanning electron microscope findings. Dent Mater10:150-155.
12.
Gwinnett AJ, Tay FR, Pang KM, Wei SHY (1995). Quantitative contribution of the collagen network in dentin hybridization (abstract). J Dent Res74(Spec Iss):403.
13.
Hayakawa T. (1993). Adhesion between the resin composite and polished dentin without pretreatment. SJ Jpn Dent Mater12:455-465.
14.
Inokoshi S., Hosoda H., Harnirattisai C., Shimada Y. (1993). Interfacial structure between dentin and seven dentin bonding systems revealed using argon ion beam etching. Oper Dent18:8-16.
15.
Jacobsen T., Finger WJ (1993). Morphology of coupling sites between bonding agents and dentine in vivo and in vitro. J Dent21:150-157.
16.
Kiyomura M. (1987). Bonding strength to bovine dentin with 4-META/MMA-TBB resin-long-term stability and influence of water. SJ Jpn Dent Mater6:860-872.
17.
Kramer IRH, McLean JW (1952). Alterations in the staining reaction of dentine resulting from a constituent of a new self-polymerising resin. Br Dent J93:150-153.
18.
Manabe A., Katsuno K., Itoh K., Wakumoto S., Miyasaka T. (1991). Bonding efficacy of erythritol methacrylate solutions as dentin primers. J Dent Res70:1294-1298.
19.
Marshall GW Jr, Balooch N., Tench RJ, Kinney JH, Marshall SJ (1993). Atomic force microscopy of acid effects on dentin . Dent Mater9:265-268.
20.
Misra DN (1989). Adsorption of 4-methacryloyloxyethyl trimellitate anhydride (4-META) on hydroxyapatite and its role in composite bonding. J Dent Res68:42-47.
21.
Morra M. (1993). Acid-base properties of adhesive dental polymers . Dent Mater9:375-378.
22.
Munksgaard EC, Asmussen E. (1984). Bonding strength between dentin and restorative resins mediated by mixtures of HEMA and glutaraldehyde. J Dent Res63:1087-1089.
23.
Nakabayashi N. (1992). The hybrid layer: A resin-dentin composite. Proc Finn Dent Soc88:321-329.
24.
Nakabayashi N. (1993). Dental biomaterials. In: Biomedical application of polymeric materials. Tsuruta T, Hayashi T, Kataoka K, Ishihara K, Kimura Y, editors. Boca Raton, FL: CRC Press, pp. 220-255.
25.
Nakabayashi N. (1995). Bonding to dentin with minimal tensile bond strength deviation (abstract). J Dent Res74(Spec Iss):413.
26.
Nakabayashi N., Watanabe A. (1983). SEM and TEM observation of dentin surface treated for adhesion. Rep Inst Med Dent Eng17:45-55.
27.
Nakabayashi N., Takarada K. (1992). Effect of HEMA on bonding to dentin. Dent Mater8:125-130.
28.
Nakabayashi N., Kojima K., Masuhara E. (1982). The promotion of adhesion by the infiltration of monomers into tooth substrates. J Biomed Mater Res16:265-273.
29.
Nakabayashi N., Watanabe A., Gendusa NJ (1992a). Dentin adhesion of "modified" 4-META/MMA-TBB resin: function of HEMA. Dent Mater8:259-264.
30.
Nakabayashi N., Ashizawa M., Nakamura M. (1992b). Identification of a resin-dentin hybrid layer in vital human dentin created in vivo: Durable bonding to vital dentin. Quintessence Int23:135-141.
31.
Nakabayashi N., Watanabe A., Ikeda W. (1995a). Intraoral bonding of 4-META/MMA-TBB resin to vital human dentin. Am J Dent8:37-42.
32.
Nakabayashi N., Fujii B., Horiuchi H., Ishikawa I., Suda H., Yamamoto T., et al. (1995b). Occlusion of dentinal tubules with reactive polymer emulsion-In vitro evaluation with SEM observation of treated surfaces . Jpn J Conserv Dent38:1538-1548.
33.
Nishiyama N., Asakura T., Suzuki K., Horie K., Nemoto K. (1995). Effects of structural change in collagen upon binding to conditioned dentin studied by 13C NMR. J Biomed Mater Res29:107-111.
34.
Pashley DH (1991). Dentin bonding: overview of the substrate with respect to adhesive material. J Esthet Dent3:46-50.
35.
Pashley DH, Ciucchi B., Sano H., Horner JA (1993). Permeability of dentin to adhesive agents. Quintessence Int24:618-631.
36.
Perinka L., Sano H., Hosoda H. (1992). Dentin thickness, hardness, and Ca-concentration vs. bond strength of dentin adhesives. Dent Mater8:229-233.
37.
Prati C., Biagini G., Rizzoli C., Nucci C., Zucchini C., Montanari G. (1990). Shear bond strength and SEM evaluation of dentinal bonding systems. Am J Dent3:283-288.
38.
Saito Y., Hotta K., Soma R., Nakabayashi N. (1993). Effect of phosphoric acid concentration on adhesion to polished human enamel surface. Shika Zairyo12:418-423.
39.
Sano H., Shono T., Takatsu T., Hosoda H. (1994). Microporous dentin zone beneath resin impregnated layer. Oper Dent19:59-64.
40.
Sasazaki H. (1985). Adaptation of adhesive composite resin to dentinal wall. 1. Bond strength and appearance of gap. Jpn J Conserv Dent28:452-478.
41.
Suzuki K., Takahashi M., Nakai H. (1990). Treatment of dentin by aqueous solution of amino acid derivative-HEMA. Adhes Dent8:43-51.
42.
Takarada K., Kojima M., Ishihara K., Nakabayashi N. (1990). Durability of bonding between 4-META/MMA-TBB resin to dentin pretreated with 10-3. The effect of 10-3 pretreating period and subsequent glutaraldehyde treatment. J Jpn Dent Mater9:831-840.
43.
Takarada K. (1990). Stable adhesion to dentin-Combination of EDTA 3-2 (NH4/Fe) pretreatment and 2% 4-META/MMA-TBB resin. J Jpn Dent Mater9:841-849.
44.
Titley K., Chernecky R., Maric B., Valiquette N., Smith D. (1994). The morphology of the demineralized layer in primed dentin. Am J Dent7:22-26.
45.
Toida T., Watanabe A., Nakabayashi N. (1995). Effect of smear layer on bonding to dentin prepared with bur. J Jpn Dent Mater14:109-116.
46.
Van Meerbeek B., Inokoshi S., Braem M., Lambrechts P., Van Herle G. (1992). Morphological aspects of the resin-dentin interdiffusion zone with different dentin adhesive systems. J Dent Res71:1530-1540.
47.
Van Meerbeek B., Dhem A., Goret-Nicaise N., Braem M., Lambrechts P., Van Herle G. (1993). Comparative SEM and TEM examination of the ultrastructure of the resin-dentin interdiffusion zone. J Dent Res72:495-501.
48.
Watanabe I. (1992). Photocure bonding agents to ground dentin. J Jpn Dent Mater11:955-973.
49.
Watanabe I., Takarada K., Nakabayashi N. (1991). Adhesion of 4-META/MMA-TBB resin to dentin treated with phosphoric acid. J Jpn Dent Mater10:671-677.
50.
Watanabe I., Nakabayashi N., Pashley DH (1994a). Bonding to ground dentin by a phenyl-P self-etching primer. J Dent Res73:1212-1220.
51.
Watanabe I., Saimi Y., Nakabayashi N. (1994b). Effect of smear layer on bonding to ground dentin-Relationship between grinding condition and tensile bonding strength. J Jpn Dent Mater13:101-108.
52.
Yamauchi J. (1986). Study of dental adhesive resin containing phosphoric acid methacrylate monomer. J Jpn Dent Mater5:144-154.
53.
Yu XY, Davis EL, Joynt RB, Wieczkowski G. (1991). Bond strength evaluation of a class V composite resin restoration. Quintessence Int22:391-396.
