Oral Colonization with Actinomyces Species in Infants by Two Years of Age

Abstract

In early childhood, the human mouth is already colonized by actinomycetes. Due to recent taxonomic changes within the genus Actinomyces, up-to-date data are warranted on the time and succession of different Actinomyces species in the oral cavity. By using a longitudinal study design and culture techniques, we examined the age-related occurrence of Actinomyces species in saliva from 39 healthy infants at 2, 6, 12, 18, and 24 months of age. Altogether 428 Actinomyces isolates were available for this study. Identification was based on biochemical tests and gas chromatographic demonstration of metabolic end-products, and when needed, cellular fatty acid profiles were determined. The frequency of the total actinomycetal flora increased from 31% to 97% within 2 years. A. odontolyticus was the most prominent Actinomyces colonizer at all five sampling occasions. A. naeslundii was the second most common Actinomyces sp. but was not detected before the age of 1 year. As a novel observation, we found A. graevenitzii in the oral cavity. The number of A. graevenitzii isolates indicates that this species is not just occasionally present in infants' mouths. We also found A. viscosus, A. gerencseriae, A. israelii, and A. georgiae. Based on the present results, we suggest that A. odontolyticus is the main primary Actinomyces species on oral mucosal surfaces in infants up to 2 years of age.

Keywords

Actinomyces spp.infant mouth oral colonization.

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References

Bowden GH (1990). Microbiology of root surface caries in humans . J Dent Res 69:1205-1210.

Bowden GH (1997). Does assessment of microbial composition of plaque/saliva allow for diagnosis of disease activity of individuals? Community Dent Oral Epidemiol 25:76-81.

Boyar RM , Bowden GH (1985). The microflora associated with the progression of incipient carious lesions in teeth of children living in a water-fluoridated area. Caries Res 19:298-306.

Cisar JO , Sandberg AL , Clark WB (1989). Molecular aspects of adherence of Actinomyces viscosus and Actinomyces naeslundii to oral surfaces. J Dent Res 68(Spec Iss):1558-1559.

Cole MF , Bryan S. , Evans MK , Pearce CL , Sheridan MJ , Sura PA , et al. (1998). Humoral immunity to commensal oral bacteria in human infants: salivary antibodies reactive with Actinomyces naeslundii genospecies 1 and 2 during colonization. Infect Immun 66:4283-4289.

Crowley PJ , Fischlschweiger W. , Coleman SE , Bleiweis AS (1987). Intergeneric bacterial coaggregations involving mutans streptococci and oral actinomyces. Infect Immun 55:2695-2700.

Ellen RP , Balcerzak-Raczkowski IB (1975). Differential medium for detecting dental plaque bacteria resembling Actinomyces viscosus and Actinomyces naeslundii. J Clin Microbiol 2:305-310.

Ellen RP (1976). Establishment and distribution of Actinomyces viscosus and Actinomyces naeslundii in the human oral cavity. Infect Immun 14:1119-1124.

Ellen RP , Grove DA (1989). Bacteroides gingivalis vesicles bind to and aggregate Actinomyces viscosus. Infect Immun 57:1618-1620.

10.

Ellen RP , Sivendra R. (1985). In vitro attachment, salivary agglutination, and surface fibril density of fresh Actinomyces isolates from two distinct oral surfaces. J Dent Res 64:799-803.

11.

Funke G. , Ramos CP , Femandez-Garayzabal JF , Weiss N. , Collins MD (1995). Description of human-derived Centers for Disease Control coryneform group 2 bacteria as Actinomyces bernardiae sp. nov. Int J Syst Bacteriol 45:57-60.

12.

Funke G. , Alvarez N. , Pascual C. , Falsen E. , Akervall E. , Sabbe L. , et al. (1997). Actinomyces europaeus sp. nov., isolated from human clinical specimens. Int J Syst Bacteriol 47:687-692.

13.

Gibbons RJ , Hay DI (1988). Human salivary acidic proline-rich proteins and statherin promote the attachment of Actinomyces viscosus LY7 to apatitic surfaces . Infect Immun 56:439-445.

14.

Johnson JL , Moore LV , Kaneko B. , Moore WE (1990). Actinomyces georgiae sp. nov., Actinomyces gerencseriae sp. nov., designation of two genospecies of Actinomyces naeslundii, and inclusion of A. naeslundii serotypes II and III and Actinomyces viscosus serotype II in A. naeslundii genospecies 2. Int J Syst Bacteriol 40:273-286.

15.

Kolenbrander PE , Celesk RA (1983). Coaggregation of human oral Cytophaga species and Actinomyces israelii. Infect Immun 40:1178-1185.

16.

Kolenbrander PE , Andersen RN , Moore LV (1990). Intrageneric coaggregation among strains of human oral bacteria: potential role in primary colonization of the tooth surface . Appl Environ Microbiol 56:3890-3894.

17.

Kononen E. , Kanervo A. , Takala A. , Asikainen S. , Jousimies-Somer H. (1999). Establishment of oral anaerobes during the first year of life. J Dent Res 78:1634-1639.

18.

Liljemark WF , Bloomquist CG , Bandt CL , Pihlstrom BL , Hinrichs JE , Wolff LF (1993). Comparison of the distribution of Actinomyces in dental plaque on inserted enamel and natural tooth surfaces in periodontal health and disease. Oral Microbiol Immunol 8:5-15.

19.

Moore WE , Moore LV (1994). The bacteria of periodontal diseases. Periodontol 2000 5:66-77.

20.

Nesbitt WE , Fukushima H. , Leung KP , Clark WB (1993). Coaggregation of Prevotella intermedia with oral Actinomyces species. Infect Immun 61:2011-2014.

21.

Nyvad B. , Kilian M. (1987). Microbiology of the early colonization of human enamel and root surfaces in vivo. Scand J Dent Res 95:369-380.

22.

Putnins EE , Bowden GH (1993). Antigenic relationships among oral Actinomyces isolates, Actinomyces naeslundii genospecies 1 and 2, Actinomyces howellii, Actinomyces denticolens, and Actinomyces slackii. J Dent Res 72:1374-1385.

23.

Ramos CP , Falsen E. , Alvarez N. , Akervall E. , Sjoden B. , Collins MD (1997). Actinomyces graevenitzii sp. nov., isolated from human clinical specimens. Int J Syst Bacteriol 47:885-888.

24.

Sarkonen N. , Kononen E. , Summanen P. , Kononen M. , Jousimies-Somer H. (1999). Growth characteristics and identification of Actinomyces species (abstract). J Dent Res 78(Spec Iss):344.

25.

Schaal KP , Lee HJ (1992). Actinomycete infections in humans-a review. Gene 115:201-211.

26.

Skopek RJ , Liljemark WF , Bloomquist CG , Rudney JD (1993). Dental plaque development on defined streptococcal surfaces. Oral Microbiol Immunol 8:16-23.

27.

Summanen P. , Baron EJ , Citron DM , Strong CA , Wexler HM , Finegold SM (1993). Wadsworth anaerobic bacteriology manual. 5th ed. Belmont, CA: Star Publishing.

28.

Wust J. , Stubbs S. , Weiss N. , Funke G. , Collins MD (1995). Assignment of Actinomyces pyogenes-like (CDC coryneform group E) bacteria to the genus Actinomyces as Actinomyces radingae sp. nov. and Actinomyces turiciensis sp. nov. Lett Appl Microbiol 20:76-81.

29.

Zylber LJ , Jordan HV (1982). Development of a selective medium for detection and enumeration of Actinomyces viscosus and Actinomyces naeslundii in dental plaque. J Clin Microbiol 15:253-259.