This technic decreases the time required for MPS (mucopolysaccharide) testing, and per mits quantitative measurement of urinary MPS without the need for elaborate electrophoretic and chromato graphic equipment. The method, if supplemented by a differential chromato graphic study, helps identify specific fractions of the MPS components. These two screening methods are useful for confirmation of clinical diagnosis of various mucopolysaccharidoses.
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References
1.
Berry, H.K. and Spinanger, J.J.: A paper spot test useful in study of Hurler's syndrome. J. Lab. Clin. Med.55: 136, 1960.
2.
Dorfman, A. and Ott, M.L.: A turbidimimetric method for the assay of hyaluronidase. J. Biol. Chem.172: 367, 1948.
3.
Carter, C.H. , Wan, A.T. and Carpenter , D.G.: Commonly used tests in the detection of Hurler's syndrome. J. Pediat.73: 217, 1968.
4.
Renuart, A.W. : Screening for inborn errors of metabolism associated with mental deficiency or neurologic disorders or both. New Eng. J. Med.274: 384, 1966.
5.
McDonald, T.P. : Effects of vitamin A on mucopolysaccharides of rat blood platelets. Amer. J. Physiol.210: 807, 1966 .
6.
Castor, C.W. and Dorsetwitz, E.L.: Identification of acid mucopolysaccharides by paper chromatography. J. Chromatog.13: 157, 1964.
7.
Carson, N.A.J. and Neill, D.W.: Metabolic abnormalities detected in a survey of mentally backward individuals in Northern Ireland. Arch. Dis. Child.37: 505, 1962.
8.
Procopis, P.G. , Turner, B., Ruxton , J.T. and Brown, D.A.: Screening tests for mucopolysaccharidosis . J. Ment. Defic. Res.12: 13, 1968.
9.
Dische, Z.: A new specific color reaction of hexuronic acid. J. Biol. Chem.167: 189, 1947.