Abstract
The influence of the glucose concentration in urines being assayed as part of a pancreolauryl test was investigated. Paired patient urines (n = 5) were assayed at 60° and 70°C, before and after spiking to 5% glucose. The influence of assay temperature alone was assessed using glucose-free paired patient urines (n = 10). Aqueous glucose solutions and spiked (5% glucose) normal random urines (n = 5) were assayed to assess the effect of glucose concentration alone.
There was no difference in T/K ratios for glucose-free patient samples at 60°, 70° and 80°C. After spiking with glucose T/K ratios were significantly different when assayed at 70°C, but not when assayed at 60°C. Aqueous glucose solutions ≥ 0.6% produced a pigment at temperatures ≥ 65°C which absorbed at the λmax of fluorescein.
Glucose was found to interfere in the pancreolauryl test. Caution should be exercised when interpreting results from glucosuric samples.